Recombinant coho salmon insulin‐like growth factor I

Moriyama, Shunsuke; Duguay, Stephen J.; Conlon, J. Michael; Duan, Cunming; Dickhoff, Walton W.; Plisetskaya, Erika M.

doi:10.1111/j.1432-1033.1993.tb18366.x

Cited by 34 publications

(10 citation statements)

References 33 publications

(21 reference statements)

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“…This might be due to the fact that we used rhIGFs, which are 80% homologous to teleost IGFs [9]. In a study carried out on coho salmon, Moriyama et al [10] found that the IGF-I plasma levels vary between 45.2 Ϯ 5.4 and 117.4 Ϯ 19.1 ng/ml, placing our effective concentrations within the range found in the plasma of another teleost species and confirming that the positive effects of IGFs observed in our experiment are not pharmacological.…”

Section: Discussionmentioning

confidence: 96%

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Recombinant Human Insulin-Like Growth Factor I Stimulates All Stages of 11-Ketotestosterone-Induced Spermatogenesis in the Japanese Eel, Anguilla japonica, In Vitro1

Nader¹,

Miura²,

Ando³

et al. 1999

Biology of Reproduction

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In this study, we examined the in vitro effects of insulin-like growth factor I (IGF-I) in the presence or absence of 11-ketotestosterone (11-KT: the spermatogenesis-inducing hormone) on the proliferation of Japanese eel (Anguilla japonica) testicular germ cells. Initially, a short-term culture (15 days) of testicular tissue with only type A and early type B spermatogonia (preproliferated spermatogonia) was carried out in Leibovitz-15 growth medium supplemented with different concentrations of recombinant human IGF (rhIGF)-I or -II in the presence or absence of 10 ng/ml of 11-KT. Late type B spermatogonia (proliferated spermatogonia) were observed in treatments of 100 ng/ml of both rhIGF-I and -II in combination with 11-KT, indicating the onset and progression of spermatogenesis. In all tested rhIGF-I concentrations (except 0.1 ng/ml) supplemented with 11-KT, late type B spermatogonia were detected in at least one individual. Then, we proceeded with an in vitro 45-day culture of testicular tissue with 100 ng/ml of rhIGF-I in the presence or absence of 10 ng/ml of 11-KT to test the long-term effects of rhIGF-I on the spermatogenetic cycle. The presence of all types of germ cells, including spermatozoa, in the testis cultured with the admixture of the two hormones indicated that the germ cells underwent complete spermatogenesis whereas no germ cell proliferation was observed when the rhIGF-I was applied alone. These results suggest that IGF-I in the presence of 11-KT plays an essential role in the onset, progress, and regulation of spermatogenesis in the testis of the Japanese eel.

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Section: Discussionmentioning

confidence: 96%

“…Both IGF-I and -II are mitogenic peptide hormones that play an important role in the regulation of growth, differentiation, regeneration, and metabolism [7]. IGF-I is mainly produced in the liver [8][9][10] and has been known for some time to mediate most of the growth-promoting actions of growth hormone (GH) [11][12][13].…”

Section: Introductionmentioning

confidence: 99%

Recombinant Human Insulin-Like Growth Factor I Stimulates All Stages of 11-Ketotestosterone-Induced Spermatogenesis in the Japanese Eel, Anguilla japonica, In Vitro1

Nader¹,

Miura²,

Ando³

et al. 1999

Biology of Reproduction

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“…However, the role of IGFs in these species has not been extensively characterised and it remains to be determined if IGFs have been functionally as well as structurally conserved throughout vertebrate evolution. While in vitro characterisation of salmon (Moriyama et al 1993), trout (Moriyama et al 1995) and chicken (Dawe et al 1988, Armstrong et al 1990, Kallincos et al 1990, Upton et al 1992) IGF-I has been described, very few in vivo studies using homologous proteins have been performed. Marsupials (metatherians) diverged most recently from placental mammals (eutherians) and are thought to have potential as developmental models (Tyndale-Biscoe & Janssens 1988).…”

Section: Introductionmentioning

confidence: 99%

Purification, amino acid sequence and characterisation of kangaroo IGF-I

Yandell

Francis²,

Wheldrake³

et al. 1998

Journal of Endocrinology

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Insulin-like growth factor-I (IGF-I) and IGF-II have been purified to homogeneity from kangaroo (Macropus fuliginosus) serum, thus this represents the first report of the purification, sequencing and characterisation of marsupial IGFs. N-Terminal protein sequencing reveals that there are six amino acid differences between kangaroo and human IGF-I. Kangaroo IGF-II has been partially sequenced and no differences were found between human and kangaroo IGF-II in the 53 residues identified. Thus the IGFs appear to be remarkably structurally conserved during mammalian radiation. In addition, in vitro characterisation of kangaroo IGF-I demonstrated that the functional properties of human, kangaroo and chicken IGF-I are very similar. In an assay measuring the ability of the proteins to stimulate protein synthesis in rat L6 myoblasts, all IGF-I proteins were found to be equally potent. The ability of all three proteins to compete for binding with radiolabelled human IGF-I to type-1 IGF receptors in L6 myoblasts and in Sminthopsis crassicaudata transformed lung fibroblasts, a marsupial cell line, was comparable. Furthermore, kangaroo and human IGF-I react equally in a human IGF-I RIA using a human reference standard, radiolabelled human IGF-I and a polyclonal antibody raised against recombinant human IGF-I. This study indicates that not only is the primary structure of eutherian and metatherian IGF-I conserved, but also the proteins appear to be functionally similar.

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“…But the impact of molecular genetics on fisheries will not stop there. Our ability to examine and manipulate molecules has increased phenomenally -if we choose, we can even make transgenic fish (Devlin, 1993), or express recombinant fish proteins (Moriyama et aL, 1993). Research using these techniques can help elucidate the roles of various genes in reproduction, growth, etc.…”

Section: The Future Of Molecular Genetics In Fisheriesmentioning

confidence: 99%