Recombinant capripoxviruses expressing proteins of bluetongue virus: Evaluation of immune responses and protection in small ruminants

Perrin, Aurélie; Albina, Emmanuel; Bréard, Emmanuel; Sailleau, Corinne; Promé, Sylvain; Grillet, Colette; Kwiatek, Olivier; Russo, Pierre; Thiéry, Richard; Zientara, Stéphan; Cêtre-Sossah, Catherine

doi:10.1016/j.vaccine.2007.06.052

Cited by 75 publications

(45 citation statements)

References 43 publications

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“…Inactivated vaccines have the associated disadvantages of needing larger doses to raise an immune response and the requirement for every batch to be tested to ensure complete inactivation of infectivity. Thus, a variety of alternate approaches have been undertaken to generate BTV vaccines, many of which have been tested in laboratory conditions with some level of success (1,13,14,21,30,32). In fact, our laboratory has demonstrated strong evidence supporting the VLPs as a good alternative to current vaccines (9,28,30,32,33).…”

Section: Discussionmentioning

confidence: 99%

Generation of Replication-Defective Virus-Based Vaccines That Confer Full Protection in Sheep against Virulent Bluetongue Virus Challenge

Matsuo

Celma

Boyce

et al. 2011

J Virol

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The reverse genetics technology for bluetongue virus (BTV) has been used in combination with complementing cell lines to recover defective BTV-1 mutants. To generate a potential disabled infectious single cycle (DISC) vaccine strain, we used a reverse genetics system to rescue defective virus strains with large deletions in an essential BTV gene that encodes the VP6 protein (segment S9) of the internal core. Four VP6-deficient BTV-1 mutants were generated by using a complementing cell line that provided the VP6 protein in trans. Characterization of the growth properties of mutant viruses showed that each mutant has the necessary characteristics for a potential vaccine strain: (i) viral protein expression in noncomplementing mammalian cells, (ii) no infectious virus generated in noncomplementing cells, and (iii) efficient replication in the complementing VP6 cell line. Further, a defective BTV-8 strain was made by reassorting the two RNA segments that encode the two outer capsid proteins (VP2 and VP5) of a highly pathogenic BTV-8 with the remaining eight RNA segments of one of the BTV-1 DISC viruses. The protective capabilities of BTV-1 and BTV-8 DISC viruses were assessed in sheep by challenge with specific virulent strains using several assay systems. The data obtained from these studies demonstrated that the DISC viruses are highly protective and could offer a promising alternative to the currently available attenuated and killed virus vaccines and are also compliant as DIVA (differentiating infected from vaccinated animals) vaccines.

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Section: Discussionmentioning

confidence: 99%

Generation of Replication-Defective Virus-Based Vaccines That Confer Full Protection in Sheep against Virulent Bluetongue Virus Challenge

Matsuo

Celma

Boyce

et al. 2011

J Virol

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“…More recently, a canarypoxbased vector that expressed optimized synthetic genes for VP2 and VP5 (two injections, 22 days apart) elicited high levels of neutralizing antibodies, a differential reactivity to VP7 as compared to sera from infected sheep (DIVA), and strong protection against homologous challenge (BTV-17); such a non replicative canarypox vector is extensively and safely used over the world in other recombinant vaccines [10]. Finally, a replicative capripox encoding for VP2, VP7, NS1 and NS3 (one injection) was partially protective in sheep [78,99]. Thus, recombinant vectors can provide protective immunity with DIVA properties but their efficacy barely reaches that of inactivated vaccines, still requiring several injections for efficient long-term protection.…”

Section: Recombinant Vectorsmentioning

confidence: 99%

Bluetongue virus: virology, pathogenesis and immunity

et al. 2008

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-Bluetongue (BT) virus, an orbivirus of the Reoviridae family encompassing 24 known serotypes, is transmitted to ruminants via certain species of biting midges (Culicoides spp.) and causes thrombo-hemorrhagic fevers mainly in sheep. During the 20th century, BTV was endemic in sub-tropical regions but in the last ten years, new strains of BTV (serotypes 1, 2, 4, 8, 9, 16) have appeared in Europe leading to a devastating disease in naive sheep and bovine herds (serotype 8). BTV enters into insect cells via the viral inner core VP7 protein and in mammalian cells via the external capsid VP2 haemagglutinin, which is the major determinant of BTV serotype and neutralization. BTV replicates in mononuclear phagocytes and endothelial cells where it induces expression of inflammatory cytokines as well as apoptosis. BTV can remain as nonreplicating entities concealed in erythrocytes for up to five months. Homologous protection against one BTV serotype involves neutralizing antibodies and T cell responses directed to the external VP2 and VP5 proteins, whereas heterologous protection is supported by T cells directed to the NS1 non structural protein and inner core proteins.

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“…The common immunogenic properties of these viruses have been used for the preparation of live attenuated vaccines that protect all ruminants against CaPV infection (Kitching et al, 1987). Recombinant CaPVs have also been developed for multivalent vaccination purposes (Berhe et al, 2003;Perrin et al, 2007;Romero et al, 1993;Wade-Evans et al, 1996;Wallace et al, 2006). However, although they are antigenically closely related, restriction enzyme pattern analysis, cross-hybridization studies and, more recently, nucleic acid sequencing have shown that nearly all CaPVs can be grouped according to their host origins Cao et al, 1995;Gershon & Black, 1988;Kitching et al, 1989;Tulman et al, 2002).…”

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Capripoxvirus G-protein-coupled chemokine receptor: a host-range gene suitable for virus animal origin discrimination

Goff

Lamien

Fakhfakh

et al. 2009

Journal of General Virology

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The genus Capripoxvirus within the family Poxviridae comprises three closely related viruses, namely goat pox, sheep pox and lumpy skin disease viruses. This nomenclature is based on the animal species from which the virus was first isolated, respectively, goat, sheep and cattle. Since capripoxviruses are serologically identical, their specific identification relies exclusively on the use of molecular tools. We describe here the suitability of the G-protein-coupled chemokine receptor (GPCR) gene for use in host-range grouping of capripoxviruses. The analysis of 58 capripoxviruses showed three tight genetic clusters consisting of goat pox, sheep pox and lumpy skin disease viruses. However, a few discrepancies exist with the classical virus-host origin nomenclature: a virus isolated from sheep is grouped in the goat poxvirus clade and vice versa. Intra-group diversity was further observed for the goat pox and lumpy skin disease virus isolates. Despite the presence of nine vaccine strains, no genetic determinants of virulence were identified on the GPCR gene. For sheep poxviruses, the addition or deletion of 21 nucleic acids (7 aa) was consistently observed in the 59 terminal part of the gene. Specific signatures for each cluster were also identified. Prediction of the capripoxvirus GPCR topology, and its comparison with other known mammalian GPCRs and viral homologues, revealed not only a classical GPCR profile in the last three-quarters of the protein but also unique features such as a longer N-terminal end with a proximal hydrophobic a-helix and a shorter serine-rich C-tail.3These authors contributed equally to this work.Two supplementary tables are available with the online version of this paper.Journal of General Virology (2009), 90, 1967-1977 DOI 10.1099 Davies, 1981Davies, , 1982Diallo & Viljoen, 2007). CaPV infections lead to similar clinical signs in sheep and goats, mainly characterized by fever, excess salivation, conjunctivitis and rhinitis with ocular and nasal discharges. These symptoms are followed by eruption of pox lesions throughout the skin. LSD is a subacute to acute cattle disease with appearance of skin nodules. During epizootics of CaPVs in domestic animals, disease in wild ungulates has never been reported (Davies, 1991), although serology and isolated cases suggests that wildlife species have a possible role in virus maintenance.CaPVs are generally considered to be host-specific, leading to outbreaks in one preferred host. This is partially true since some SPPV and GTPV isolates are capable of causing severe diseases in both sheep and goats (Davies, 1976;Kitching et al., 1989). An in-depth epidemiological investigation to specifically identify the viruses involved in acute small ruminant pox diseases cannot be achieved by serological testing alone due to the very close antigenic relationship between CaPVs, with the existence of only one serotype (Kitching et al., , 1989. The common immunogenic properties of these viruses have been used for the preparation of live attenuated vaccines that ...

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Recombinant capripoxviruses expressing proteins of bluetongue virus: Evaluation of immune responses and protection in small ruminants

Cited by 75 publications

References 43 publications

Generation of Replication-Defective Virus-Based Vaccines That Confer Full Protection in Sheep against Virulent Bluetongue Virus Challenge

Generation of Replication-Defective Virus-Based Vaccines That Confer Full Protection in Sheep against Virulent Bluetongue Virus Challenge

Bluetongue virus: virology, pathogenesis and immunity

Capripoxvirus G-protein-coupled chemokine receptor: a host-range gene suitable for virus animal origin discrimination

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