Recombinant Bovine Adenovirus-3 Co-Expressing Bovine Respiratory Syncytial Virus Glycoprotein G and Truncated Glycoprotein gD of Bovine Herpesvirus-1 Induce Immune Responses in Cotton Rats

Brownlie, Robert; Kumar, Pankaj; Babiuk, Lorne A.; Tikoo, Suresh K.

doi:10.1007/s12033-014-9801-x

Cited by 9 publications

(9 citation statements)

References 27 publications

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“…Ten pools of five randomly selected BRD samples were assembled using 32 l from each sample. The resulting 160-l pools were passed through a 200-nm filter, and the resulting filtrates were incubated for 1.5 h in a cocktail of DNase and RNase enzymes (53). Nucleic acids were then extracted using the QIAquick viral RNA column purification system according to the manufacturer's instructions.…”

Section: Methodsmentioning

confidence: 99%

A Metagenomics and Case-Control Study To Identify Viruses Associated with Bovine Respiratory Disease

Kondov

Deng

et al. 2015

J Virol

181

221

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Bovine respiratory disease (BRD) is a common health problem for both dairy and beef cattle, resulting in significant economic loses. In order to identify viruses associated with BRD, we used a metagenomics approach to enrich and sequence viral nucleic acids in the nasal swabs of 50 young dairy cattle with symptoms of BRD. Following deep sequencing, de novo assembly, and translated protein sequence similarity searches, numerous known and previously uncharacterized viruses were identified. Bovine adenovirus 3, bovine adeno-associated virus, bovine influenza D virus, bovine parvovirus 2, bovine herpesvirus 6, bovine rhinitis A virus, and multiple genotypes of bovine rhinitis B virus were identified. The genomes of a previously uncharacterized astrovirus and picobirnaviruses were also partially or fully sequenced. Using real-time PCR, the rates of detection of the eight viruses that generated the most reads were compared for the nasal secretions of 50 animals with BRD versus 50 location-matched healthy control animals. Viruses were detected in 68% of BRD-affected animals versus 16% of healthy control animals. Thirtyeight percent of sick animals versus 8% of controls were infected with multiple respiratory viruses. Significantly associated with BRD were bovine adenovirus 3 (P < 0.0001), bovine rhinitis A virus (P ‫؍‬ 0.005), and the recently described bovine influenza D virus (P ‫؍‬ 0.006), which were detected either alone or in combination in 62% of animals with BRD. A metagenomics and realtime PCR detection approach in carefully matched cases and controls can provide a rapid means to identify viruses associated with a complex disease, paving the way for further confirmatory tests and ultimately to effective intervention strategies. IMPORTANCEBovine respiratory disease is the most economically important disease affecting the cattle industry, whose complex root causes include environmental, genetics, and infectious factors. Using an unbiased metagenomics approach, we characterized the viruses in respiratory secretions from BRD cases and identified known and previously uncharacterized viruses belonging to seven viral families. Using a case-control format with location-matched animals, we compared the rates of viral detection and identified 3 viruses associated with severe BRD signs. Combining a metagenomics and case-control format can provide candidate pathogens associated with complex infectious diseases and inform further studies aimed at reducing their impact. Bovine respiratory disease (BRD) is the most common and costly problem in the cattle industry, accounting for 70 to 80% of morbidity and 40 to 50% of mortality in U.S. feedlots (1, 2). The cattle industry is one of the largest agricultural sectors of the United States economy, with approximately three-quarters of a million farms raising cattle. The annual costs of BRD have been estimated at over 1 billion dollars per year (3, 4).In BRD, bacterial infections are thought to be opportunistic infections precipitated by viral infections causing damage to t...

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Section: Methodsmentioning

confidence: 99%

A Metagenomics and Case-Control Study To Identify Viruses Associated with Bovine Respiratory Disease

Kondov

Deng

et al. 2015

J Virol

181

221

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“…Moreover, two intranasal immunizations, four weeks apart of calves with single recombinant BAdV-3 expressing BHV-1 gDt and BRSV gG induced significant neutralizing antibodies against BHV-1 and BRSV in cotton rats [72]. Thus, the construction of single recombinant BAdV-3 with multiple vaccine antigens is feasible to induce virus specific immunity for protection from multiple pathogens in calves [72].…”

Section: Recombinant Badv-3 Expressing Two Vaccine Antigensmentioning

confidence: 99%

“…Since veterinary vaccines have to be safe and cost effective, the development of a recombinant BAdV-3 vectored vaccine providing immunity to more than one pathogen is desirable. Recently, we demonstrated that single recombinant BAdV-3 expressing vaccine antigens from two respiratory pathogens (gDt of BHV-1 and gG of bovine respiratory syncytial virus [BRSV]) of cattle could be isolated [72]. Moreover, two intranasal immunizations, four weeks apart of calves with single recombinant BAdV-3 expressing BHV-1 gDt and BRSV gG induced significant neutralizing antibodies against BHV-1 and BRSV in cotton rats [72].…”

Section: Recombinant Badv-3 Expressing Two Vaccine Antigensmentioning

confidence: 99%

“…Recently, we demonstrated that single recombinant BAdV-3 expressing vaccine antigens from two respiratory pathogens (gDt of BHV-1 and gG of bovine respiratory syncytial virus [BRSV]) of cattle could be isolated [72]. Moreover, two intranasal immunizations, four weeks apart of calves with single recombinant BAdV-3 expressing BHV-1 gDt and BRSV gG induced significant neutralizing antibodies against BHV-1 and BRSV in cotton rats [72]. Thus, the construction of single recombinant BAdV-3 with multiple vaccine antigens is feasible to induce virus specific immunity for protection from multiple pathogens in calves [72].…”

Section: Recombinant Badv-3 Expressing Two Vaccine Antigensmentioning

confidence: 99%

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Bovine adenovirus-3 as a vaccine delivery vehicle

Ayalew

Kumar

Gaba

et al. 2015

Vaccine

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The use of vaccines is an effective and relatively inexpensive means of controlling infectious diseases, which cause heavy economic losses to the livestock industry through animal loss, decreased productivity, treatment expenses and decreased carcass quality. However, some vaccines produced by conventional means are imperfect in many respects including virulence, safety and efficacy. Moreover, there are no vaccines for some animal diseases. Although genetic engineering has provided new ways of producing effective vaccines, the cost of production for veterinary use is a critical criterion for selecting the method of production and delivery of vaccines. The cost effective production and intrinsic ability to enter cells has made adenovirus vectors a highly efficient tool for delivery of vaccine antigens. Moreover, adenoviruses induce both humoral and cellular immune responses to expressed vaccine antigens. Since nonhuman adenoviruses are species specific, the development of animal specific adenoviruses as vaccine delivery vectors is being evaluated. This review summarizes the work related to the development of bovine adenovirus-3 as a vaccine delivery vehicle in animals, particularly cattle.

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“…129 A BAdV-3 vector (BAV851) coexpressing bovine respiratory syncytial virus G and BHV-1gDt proteins was generated, and vaccination of cotton rats with this BAV851 induced strong antigen-specific immune responses. 130 In order to evaluate whether a BAdV vector can effectively elude high levels of preexisting HAdV-5 vector immunity, naïve and HAdV-5-primed mice were immunized with BAd-H5HA (a BAdV vector expressing HA of a H5N1 influenza virus). 131 Even in the presence of very high levels of HAdV-5-specific neutralizing antibody titer (2133 ± 660), no reductions in HA-specific humoral and CMI responses were observed in mice immunized with BAd-H5HA.…”

Section: Bovine Adenovirus-based Vectorsmentioning

confidence: 99%