Recombinant binder of sperm protein1 (rec-BSP1) as a potent fertility factor mediates its effect on spermatozoa and production of blastocysts in buffalo

Abstract

Objective: To understand the effect of recombinant BSP1 (rec-BSP1) on in vitro capacitation of sperm and fertilization study Method(s): Articles were screened for reports including rec-BSP1, Capacitation, in vitro fertilization Intervention: None Main Outcome Measure(s): Reproductive outcomes, effect on gametes and embryos Result(s): Here we report an optimization of condition for rec-BSP1 production which was used for in vitro capacitation and enhancement of buffalo embryo production. The sequence of the protein was used for multiple sequence alignment which has 99% similarity with PDC 109 protein. The expression of rec-BSP1 was carried out successfully with 1 mM IPTG at 160 C for 22 hrs and purified it in soluble form. The structure of rec-BSP1 was generated using 3D modelling and analysed its mode of binding with heparin and PC by molecular docking and the structural stability of rec-BSP1-PC and rec-BSP1-heparin complexes by using molecular dynamic (MD) simulation. The effect of rec-BSP1 was observed on in vitro capacitation of spermatozoa and buffalo blastocyst production. It was found that the rec-BSP1 enhanced the sperm motility at a concentration of 50 microg/ml for 1 h of incubation without having any detrimental effect on the sperm morphology and a significant (P<0001) increase in blastocyst production at a concentration of 50 microg/ml rec-BSP1. Hence this finding represents a new insight and advance the prospective approach to developing a potential fertility factor in reproduction. Conclusion(s): The purified rec-BSP1 may affect on male fertility and mediated its effect on in vitro embryo production