Recombinant baculoviruses as mammalian cell gene-delivery vectors

BacMam System for FRET-Based cAMP Sensor Expression in Studies of Melanocortin MC1 Receptor Activation

“…9 The method employs a baculovirus vector with the CMV promoter to drive the expression of proteins in mammalian cells. This technology provides advantages such as high transduction rates; protein expression levels that can be adjusted by viral dose; low cytotoxicity to host cells; safety in production and handling (Biosafety Level 1); compatibility with a broad range of cells, including primary and stem cells; and, importantly, the ease and convenience of use (see review by Kost and Condreay 10 ). After obtaining the virus (for bacmid construction and virus production, see Materials and Methods), the conditions for cell transduction were optimized for the B16F10 cell line, relying on previous studies related to the BacMam system.…”

Astrocytic expression of transgene in the rat brain mediated by baculovirus vectors containing an astrocyte-specific promoter

“…Being insect viruses, baculoviruses are unable to replicate and express viral proteins in mammalian cells, [4][5][6][7][8] thus significantly reducing the chance of pre-existing antiviral humoral and cellular immunity in mammals.…”

“…1 Among the various gene delivery vectors that are capable of transducing glial cells is the baculovirus Autographa californica multiple nucleopolyhedrovirus (AcMNPV)-based vectors, recently emerging as a new type of gene therapy vehicles. [4][5][6][7][8] Baculoviruses infect astrocytes after being injected into the brain. 6,9 It is worth noting that the brain injection does not offer cell-type specificity: in addition to astrocytes, other types of cells in the brain, including neurons, can be transduced by the injected baculoviruses.…”

Lentiviral vectors pseudotyped with baculovirus gp64 efficiently transduce mouse cells in vivo and show tropism restriction against hematopoietic cell types in vitro

“…Baculovirus (Autographa californica nuclear polyhedrosis virus, AcNPV) is an enveloped insect virus that is being developed as a vector for gene therapy. 16 Early studies showed that baculovirus vectors could efficiently transduce human hepatocyte cell lines and explanted human liver tissue. 17,18 Subsequently, it was demonstrated that baculovirus vectors could enter a broader variety of human cell types.…”