Recombinant bacteriophages as gold binding bio-templates

Korkmaz, Nuriye

doi:10.1016/j.colsurfb.2013.07.063

Cited by 23 publications

(24 citation statements)

References 42 publications

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“…Previously, we have investigated the effect of pH on Au surface binding affinities of fd phages by QCM measurements [23]. No significant phage binding was observed on Au sensors in a broad pH range (pH 3-pH 9).…”

Section: Discussionmentioning

confidence: 99%

“…Lately, we have shown that introducing three Met subunits to N-terminal of major coat protein p8 subunits facilitates MMM-bacteriophage binding to Au surfaces or NPs [23]. Slocik et al have obtained water stabilized dispersed AuNPs with the help of gold binding peptides identified by phage display technique [24].…”

Section: Phage Engineering and Au Surface Binding Testsmentioning

confidence: 99%

“…It was previously shown that sulfur carrying amino acids like cysteine have strong binding affinities against Au [22]. Expression of three Met residues on p8 major coat protein subunits of filamentous fd bacteriophages resulted in enhanced Au binding and metallization properties [23]. Slocik et al applied gold binding peptides (e.g.…”

Section: Introductionmentioning

confidence: 98%

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Engineering filamentous bacteriophages for enhanced gold binding and metallization properties

Zirpel

Arslan

Lee

2015

Journal of Colloid and Interface Science

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Section: Discussionmentioning

confidence: 99%

Section: Phage Engineering and Au Surface Binding Testsmentioning

confidence: 99%

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Engineering filamentous bacteriophages for enhanced gold binding and metallization properties

Zirpel

Arslan

Lee

2015

Journal of Colloid and Interface Science

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“…Phages can also be utilized in the self-assembly of nanomaterials involving inorganic molecules such as gold, [32e,53b, 58] silver, [59] titanium dioxide, [60] copper, [61] aluminum, [62] and many others. Of these metals, it has been demonstrated that filamentous phages can form networks with gold (enhanced by the presence of imidazole).…”

Section: Phage-directed Nanomaterials Synthesis and Assembly For Bmentioning

confidence: 99%

“…Of these metals, it has been demonstrated that filamentous phages can form networks with gold (enhanced by the presence of imidazole). [58a Importantly, the phages still maintained cell targeting, thus suggesting that phages can be used for both targeting and imaging owing to their ability to maintain specificity for cells while associating with inorganic gold nanoparticles. [58a Such properties are very promising for disease diagnosis and biosensing.…”

Section: Phage-directed Nanomaterials Synthesis and Assembly For Bmentioning

confidence: 99%

Phage‐Enabled Nanomedicine: From Probes to Therapeutics in Precision Medicine

2017

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Both lytic and temperate bacteriophages (phages) can be applied in nanomedicine, in particular, as nanoprobes for precise disease diagnosis and nanotherapeutics for targeted disease treatment. Since phages are bacteria-specific viruses, they do not naturally infect eukaryotic cells and are not toxic to them. They can be genetically engineered to target nanoparticles, cells, tissues, and organs, and can also be modified with functional abiotic nanomaterials for disease diagnosis and treatment. This Review will summarize the current use of phage structures in many aspects of precision nanomedicine, including ultrasensitive biomarker detection, enhanced bioimaging for disease diagnosis, targeted drug and gene delivery, directed stem cell differentiation, accelerated tissue formation, effective vaccination, and nanotherapeutics for targeted disease treatment. We will also propose future directions in the area of phage-based nanomedicines, and discuss the state of phage-based clinical trials.

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Genetically Induced In Situ‐Poling for Piezo‐Active Biohybrid Nanowires

Kilper¹,

Jahnke²,

Aulich³

et al. 2018

Advanced Materials

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ii) nonferroelectric materials. In both classes, a high macroscopic piezoelectric response, which is the average over all crystallites, is connected with the polar axes of the crystallites parallel aligned and with the same force direction. [6] In ferroelectric materials the direction of the polar axes can be permanently aligned postsynthesis by a strong electric field, which is referred as poling. [6a] However, the nonferroelectric piezo-active materials, like zinc oxide (ZnO), gallium nitride (GaN), beryllium oxide (BeO), or cadmium sulfide (CdS), do not allow the reorientation of the polar axes. In such materials, the maximum piezoelectric response of a crystallite is linked to one of its crystallographic axis (Figure 1a), which cannot be aligned postsynthesis. [5,7] In piezo-active materials the polarization is connected to the ordered alignment of electrical dipoles. [8] In order to synthesize piezo-active nonferroelectric materials either the direction of crystal growth has to be controlled [9] or the crystallite dipoles have to be aligned in a freely moveable state, e.g., in solution.To influence the deposition of inorganic materials, template structures inducing a force field were reported. Various template classes, e.g., polyelectrolytes, [10] peptides, [11] or biological entities, like viruses [12] and phages, [13] have been applied to control the morphology [14] of the inorganic phase or the mechanical performance of the hybrid material. [15] For such bioinspired synthesis approaches, M13 phage templates are of specific interest. M13 phages are nonpathogenic for humans and provide a nanowire-like structure (6 × 900 nm 2 ), which is precisely replicated in bacteria. The phage particles are robust and resistant to heat (>80 °C), mechanical stresses, and various solvents. [16] The protein coat, encapsulating the DNA genome, consists of five different proteins (p3, p5, p7, p8, and p9), which can be modified. The major coat protein p8 has dipole character with a positively charged C-terminus, interacting with the phage DNA and a negatively charged N-terminus facing the environment. [17] Due to the noncentrosymmetric arrangement of the p8 proteins, which are helically arranged around the central DNA strand, the M13 phage template represents a net dipole. [18] Moreover, the dipole strength can be further enhanced by the genetic modification of the major coat protein, which was shown in Polycrystalline piezo-active materials only exhibit a high macroscopic piezoresponse if they consist of particles with oriented crystal directions and aligned intrinsic dipole moments. For ferroelectric materials, the postsynthesis alignment of the dipoles is generally achieved by electric poling procedures. However, there are numerous technically interesting non-ferroelectric piezo-active materials like zinc oxide (ZnO). These materials demand the alignment of their intrinsic dipoles during the fabrication process. Therefore, in situ-poling techniques have to be developed. This study utilizes genetically modified M13 phage ...

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Recombinant bacteriophages as gold binding bio-templates

Cited by 23 publications

References 42 publications

Engineering filamentous bacteriophages for enhanced gold binding and metallization properties

Engineering filamentous bacteriophages for enhanced gold binding and metallization properties

Phage‐Enabled Nanomedicine: From Probes to Therapeutics in Precision Medicine

Genetically Induced In Situ‐Poling for Piezo‐Active Biohybrid Nanowires

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