2008
DOI: 10.1016/j.vaccine.2008.07.015
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Recombinant Bacillus anthracis spore proteins enhance protection of mice primed with suboptimal amounts of protective antigen

Abstract: Inactivated Bacillus anthracis spores given with protective antigen (PA) contribute to immunity against anthrax in several animal models. Antiserum raised against whole irradiated B. anthracis spores has been shown to have anti-germination and opsonic activities in vitro. Based on these observations, we hypothesized that surface-exposed spore proteins might serve as supplemental components of a PA-based anthrax vaccine. The protective anti-spore serum was tested for reactivity with recombinant forms of 30 prot… Show more

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Cited by 42 publications
(58 citation statements)
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“…Therefore, my colleagues and I have proposed a model whereby BxpB is delivered to the spore by virtue of forming a complex with BclA and possibly, to a lesser extent, with other exosporium-targeting motif sequences (58). In keeping with this, we observed a reduction in the amount of BxpB extractable from spores of a BclA-negative strain, a finding similar to that observed by others (65,66,68). However, this model is disputed by Rodenburg et al (39), based on their analysis of B. anthracis spore structure by high-resolution electron microscopy.…”
Section: Assembly Of the Exosporiumsupporting
confidence: 90%
See 1 more Smart Citation
“…Therefore, my colleagues and I have proposed a model whereby BxpB is delivered to the spore by virtue of forming a complex with BclA and possibly, to a lesser extent, with other exosporium-targeting motif sequences (58). In keeping with this, we observed a reduction in the amount of BxpB extractable from spores of a BclA-negative strain, a finding similar to that observed by others (65,66,68). However, this model is disputed by Rodenburg et al (39), based on their analysis of B. anthracis spore structure by high-resolution electron microscopy.…”
Section: Assembly Of the Exosporiumsupporting
confidence: 90%
“…Inclusion of this 133-residue protein of unknown function enhanced the efficacy of an anti-protective antigen (anti-PA) vaccine in a mouse model of infection, and anti-BAS5303 antibodies were found to increase phagocytic uptake of spores (68). Although the location of this protein has not been mapped precisely, it was reported to likely lie below the BclA nap layer on the exosporium, based on increased exposure to antibodies in the absence of BclA (68). The bas5303 determinant is monocistronic and expressed at a very late stage of sporulation (73).…”
Section: Bas5303mentioning
confidence: 99%
“…Recent studies exposed BclA as not being the only relevant immunogen of the spore and further defined its role rather as means of masking these other, possibly much more relevant spore antigens (Cybulski et al, 2008, 4927-4939, Cote et al, 2012, 1380-1392. Taking the lack of reactivity against BclA in goats into account, this might be a much more pressing issue in this animal model and should be considered in future live and acellular vaccine trials.…”
Section: Discussionmentioning
confidence: 99%
“…Although the isolated sdAbs were specific for B. anthracis, EA1 has been shown to be a spore contaminant and not an integral component of the bacterial spore [17]. The two llamas were subjected to a second series of immunizations with a combination of recombinant spore-specific proteins: BclA, gerQ, SODA1, SOD15, BxpB, and p5303 [18,19]. Centavo received a mix of all six proteins; while Whisper was immunized with a cocktail that did not include the BclA protein in an attempt to allow a stronger immune response toward the other spore proteins that may not be as immunodominant as the BclA antigen.…”
Section: Selection Of Sdabs Targeting Spore Proteinsmentioning
confidence: 99%