Recombinant attenuated bacteria for the delivery of subunit vaccines

Gentschev, Ivaylo; Dietrich, Guido; Spreng, Simone; Kolb‐Mäurer, Annette; Brinkmann, Volker; Grode, Leander; Hess, Jürgen; Kaufmann, Stefan H. E.; Goebel, Werner

doi:10.1016/s0264-410x(00)00502-8

Cited by 63 publications

(34 citation statements)

References 33 publications

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“…Lm is also capable of invading epithelial cells by a trigger mechanism using inl gene products. Therefore, Lm can be used as a vector to deliver cDNA into mammalian cells of both phagocytic and nonphagocytic cells, and this is demonstrated using cultured cells by in vitro infection (5,8).…”

Section: Discussionmentioning

confidence: 99%

“…Based on the aforementioned observations, we concluded that the intracellular pathogen Lm functions as an immune response modulator and also as a good candidate for a vector to deliver a gene into mammalian cells to modulate the acquired immune response (5,8,29). We constructed the plasmid for gene transfer into mammalian cells using Lm as described in "Materials and Methods" (Fig.…”

Section: The Gene Manipulated Lm Function To Deliver a Gene Into Mammmentioning

confidence: 99%

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Modulation of the Immune System by Listeria monocytogenes‐Mediated Gene Transfer into Mammalian Cells

Shen

Kanoh

Liu

et al. 2004

Microbiology and Immunology

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In this study, we established a method for Listeria monocytogenes (Lm)‐mediated gene transfer into mammalian cells to manipulate the immune response of the host during infection by pathogens. We used the Lm‐mediated gene transfer method in an in vivo study to manipulate host immune responses against Leishmania major (L. major)‐infection. The injection of Lm modulated the susceptible host into a resistant state against L. major‐infection. A more efficient protective effect was obtained with the injection of IL‐12‐cDNA containing Lm, and the protective effect was stronger than that of the resistant strain. The protective mechanism of Lm‐injection against L. major‐infection observed here appeared to be a result of the activation of the local immune system by the Lm‐mediated gene transfer method. The present study is the first demonstration that a gene introduced into a host by Lm works to modulate the murine host immune response against infections in vivo. Since this system strongly induces Th1 responses and suppresses Th2 responses in infected hosts, the system can be used for controlling infectious diseases and for protection against allergic responses in the future.

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Section: Discussionmentioning

confidence: 99%

Section: The Gene Manipulated Lm Function To Deliver a Gene Into Mammmentioning

confidence: 99%

Modulation of the Immune System by Listeria monocytogenes‐Mediated Gene Transfer into Mammalian Cells

Shen

Kanoh

Liu

et al. 2004

Microbiology and Immunology

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“…DC were shown to phagocytose a variety of bacterial pathogens, including Mycobacterium tuberculosis (16), Listeria monocytogenes (13,21), Salmonella enterica serovar Typhimurium (8,39), Escherichia coli (31), Yersinia pseudotuberculosis (8), Shigella flexneri (8), Borrelia burgdorferi (10), and Streptococcus gordonii (5), and we recently reported the phagocytosis of N. (22). In most cases, the phagocytosed bacteria were found to be rapidly killed by the DC.…”

Section: Discussionmentioning

confidence: 99%

Lipooligosaccharide and Polysaccharide Capsule: Virulence Factors ofNeisseria meningitidisThat Determine Meningococcal Interaction with Human Dendritic Cells

Unkmeir

Kämmerer²,

Stade

et al. 2002

Infect Immun

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In this work we analyzed the roles of meningococcal lipooligosaccharide (LOS) and capsule expression in the interaction of Neisseria meningitidis with human dendritic cells (DC). Infection of DC with serogroup B wild-type meningococci induced a strong burst of the proinflammatory cytokines and chemokines tumor necrosis factor alpha, interleukin-6 (IL-6), and IL-8. In contrast, a serogroup B mutant strain lacking LOS expression barely led to cytokine induction, demonstrating that meningococcal LOS is the main mediator of the proinflammatory response in human DC. Sialylation of meningococcal LOS did not influence cytokine secretion by DC. However, we found the phagocytosis of N. meningitidis by human DC to be inhibited by LOS sialylation. In addition, the expression of the meningococcal serogroup A, B, and C capsules dramatically reduced DC adherence of N. meningitidis and phagocytosis to some extent. Hence, LOS sialylation and capsule expression are independent mechanisms protecting N. meningitidis from the phagocytic activity of human DC.

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“…They may include improvement of the current inactivated polio vaccine by making it more efficacious in tropical countries and reducing its cost, as well as exploring entirely new approaches. These might include the development of a polio vaccine based on nonpathogenic enteric bacteria expressing engineered surface or secretable antigens or harboring viral cDNA (12,14,20,31). Hopefully, such a vaccine may turn out to be safe, inexpensive, and effective.…”

Section: Vol 77 2003 a Local Cessation Of Vaccination Against Poliomentioning

confidence: 99%

Retrospective Analysis of a Local Cessation of Vaccination against Poliomyelitis: a Possible Scenario for the Future

Короткова

Park

Cherkasova

et al. 2003

J Virol

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The global eradication of poliomyelitis will require substantial changes in immunization practices. One of the proposed scenarios includes cessation of vaccination with live oral poliovirus vaccine (OPV) and the creation of an OPV stockpile for emergency response in case of the reintroduction of poliovirus into circulation. We describe here a retrospective analysis of the cessation of OPV usage in a region of the Byelorussian Republic of the former Soviet Union in 1963 to 1966. During this period, a widespread circulation and evolution of independent lineages of vaccine-derived polioviruses took place in the region. Some of these lineages appeared to originate from OPV given to 40 children in the community during this period of essentially no vaccinations. The data demonstrate very high risks associated with both the local cessation of OPV vaccination and the proposed use of OPV to control a possible reemergence of poliovirus in the postvaccination period. The high transmissibility of OPV-derived viruses in nonimmune population, documented here, and the known existence of long-term OPV excretors should be also considered in assessing risks of the synchronized global cessation of OPV usage.The remarkable success of the Program for Global Eradication of Poliomyelitis, which has resulted in a Ͼ99% reduction of the incidence of paralytic poliomyelitis and an immense decrease in the circulation of wild polioviruses, mandates the formulation of an optimal strategy for the "endgame" (8,19,27,30) that must include both vaccination policy and emergency response measures. Among the options under consideration is the complete and coordinated cessation of vaccination with the live oral poliovirus vaccine from Sabin strains (OPV [currently the most widely used type of polio vaccine]) soon after the world is certified free from circulating wild polioviruses (32). Major advantages of this strategy are obvious: it would prevent cases of vaccine-associated paralytic poliomyelitis, which occur at a rate of one case per ϳ10 6 of the first doses of the vaccine distributed (32), and would save substantial financial and other resources that could be redirected toward solving other important public health problems. On the other hand, stopping vaccination will inevitably result in a dramatic growth in the nonimmune population and create an opportunity for a rapid spread of poliovirus should it ever be unintentionally or purposefully reintroduced into the population. This risk can only be assessed in large-scale experiments that are ethically unacceptable. However, important lessons can be learned from certain past epidemiological situations, which may be regarded as possible scenarios for the future. We describe here a retrospective analysis of such a situation. MATERIALS AND METHODSVirus isolation and preliminary characterization. For virus isolation, HeLa cells or primary cultures of human embryonic cells were used. Restriction fragment length polymorphism analysis was performed as described previously (15).DNA oligonucleotide microa...

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Recombinant attenuated bacteria for the delivery of subunit vaccines

Cited by 63 publications

References 33 publications

Modulation of the Immune System by Listeria monocytogenes‐Mediated Gene Transfer into Mammalian Cells

Modulation of the Immune System by Listeria monocytogenes‐Mediated Gene Transfer into Mammalian Cells

Lipooligosaccharide and Polysaccharide Capsule: Virulence Factors ofNeisseria meningitidisThat Determine Meningococcal Interaction with Human Dendritic Cells

Retrospective Analysis of a Local Cessation of Vaccination against Poliomyelitis: a Possible Scenario for the Future

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