Recombinant Aeromonas hydrophila outer membrane protein 48 (Omp48) induces a protective immune response against Aeromonas hydrophila and Edwardsiella tarda

Khushiramani, Rekha; Maiti, Biswajit; Shekar, Malathi; Girisha, S.K.; N, Akash; Deepanjali, Appunni; Karunasagar, Iddya

doi:10.1016/j.resmic.2012.03.001

Cited by 74 publications

(36 citation statements)

References 38 publications

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“…Outer membrane proteins have proven to be excellent targets for subunit vaccine development (Achouak et al 2001). They have not only shown remarkable immunogenic potential, anti-rOmp48 antisera of A. hydrophila have been shown to cross-react with whole cell proteins of other bacteria including A. veroni, Vibrio parahaemolyticus, Edwardsiella tarda, and E. coli (Khushiramani et al 2012). Previous studies from our laboratory have reported the immunogenic potential of the rOmpF protein of A. hydrophila.…”

Section: Discussionmentioning

confidence: 94%

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Identification and immunogenic potential of B cell epitopes of outer membrane protein OmpF of Aeromonas hydrophila in translational fusion with a carrier protein

Sharma

Dixit

2015

Appl Microbiol Biotechnol

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Aeromonas hydrophila, a ubiquitous and virulent bacterial pathogen, affects a variety of fishes, including Labeo rohita. Existing treatment strategies comprise antibiotic therapies and attenuated bacterial strain-based vaccines. No functional subunit vaccine has been available until now. Given their key role in determining pathogenicity, outer membrane proteins have been successfully explored as potential vaccine candidates. We have devised a direct strategy for eliminating non-specific responses by selectively aiming the immune response against specific immunodominant epitopes of the outer membrane protein F (OmpF) of A. hydrophila (AhOmpF). Five putative epitopes of AhOmpF predicted in silico were genetically conjugated with heat labile enterotoxin chain B of E. coli (LTB). Recombinant fusion proteins expressed in E. coli were purified from solubilized inclusion bodies and refolded. The fusion protein retained GM1 ganglioside receptor binding activity of LTB, indicating proper folding. Four of the five fusion proteins were found to be highly immunogenic. Of the four proteins, antisera against the fusion protein (anti-rEpiF1) harboring 66-80 amino acid residues of the OmpF gave maximum cross-reactivity with the targeted rOmpF in enzyme-linked immunosorbent assay (ELISA) and was able to recognize both fusion partners-rOmpF and rLTB-in Western blot. Antibody isotyping of the antisera and cytokine array analysis of the culture supernatants of splenocytes from sensitized mice manifested a mixed Th1/Th2 immune response with a bias toward Th2. Anti-rEpiF1 antibodies were able to bind to the cell membrane of live A. hydrophila cells and agglutinate them. Our results thus suggest that the OmpF epitope (66-80) in fusion with a carrier protein is a promising vaccine candidate against A. hydrophila.

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Section: Discussionmentioning

confidence: 94%

“…Outer membrane proteins have previously been identified as suitable vaccine candidates and evaluated for their immunogenic and protective potential in different bacterial species (Pal et al 1997;Wright et al 2002;Khushiramani et al 2012).…”

Section: Introductionmentioning

confidence: 99%

Identification and immunogenic potential of B cell epitopes of outer membrane protein OmpF of Aeromonas hydrophila in translational fusion with a carrier protein

Sharma

Dixit

2015

Appl Microbiol Biotechnol

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“…Administration of OmpF of A. hydrophila leads to increased IgG expression in mouse model along with increased lymphocyte proliferation and T cell activation in vitro (Yadav et al 2014 ). OmpTS of A. hydrophila was highly immunogenic in fi sh model Labeo rohita and Omp48 immunized fi sh showed survival against A. hydrophila and Edwardsiella tarda infections (Khushiramani et al 2007(Khushiramani et al , 2014. Borrelia burgdorferi is a spirochete that causes Lyme's disease.…”

Section: Porins As Vaccine Candidatesmentioning

confidence: 99%

Immuno-Modulatory Role of Porins: Host Immune Responses, Signaling Mechanisms and Vaccine Potential

Sakharwade

Prasad

Mukhopadhaya

2014

Advances in Experimental Medicine and Biology

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“…(Abraham et al, 2010) are being developed for vertical expansion while technologies like remote sensing by satellite imaging and geographical information system are being employed to promote the current seven and 15 new species (Ayyappan & Gopalakirshnan, 2008) into horizontal expansion. To tackle the diseases, experimental vaccines (Behera & Swain, 2012;Khushiramani et al, 2012) and probiotics (Parthasarathy & Ravi, 2011) are being evaluated.…”

Section: Technological Landscapementioning

confidence: 99%

Strategic Macro-Environmental Factor Analysis for Entry into the Fish Vaccines Market in India

Pallapothu

Krause

2013

IJBM

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Globally, the aquaculture segment continues to be the fastest growing food-fish producing segment and for India, the second largest food-fish producing country, it was forecasted that the segment would grow at an average annual growth rate of approximately 7.0% to reach a total production target of 8-10 million tons by 2020. Currently no commercial fish vaccines are available in India; therefore, the macro-environmental factors with respect to the competitive, political, economic, socio-cultural, technological, and legal landscapes, which influence a potential entrant's strategic business decisions to set up a fish vaccines company, are evaluated. The results indicate that although the macro-environmental factors in India are improving there are still some bureaucratic hurdles to be overcome by an entrant to assure success.

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Recombinant Aeromonas hydrophila outer membrane protein 48 (Omp48) induces a protective immune response against Aeromonas hydrophila and Edwardsiella tarda

Cited by 74 publications

References 38 publications

Identification and immunogenic potential of B cell epitopes of outer membrane protein OmpF of Aeromonas hydrophila in translational fusion with a carrier protein

Identification and immunogenic potential of B cell epitopes of outer membrane protein OmpF of Aeromonas hydrophila in translational fusion with a carrier protein

Immuno-Modulatory Role of Porins: Host Immune Responses, Signaling Mechanisms and Vaccine Potential

Strategic Macro-Environmental Factor Analysis for Entry into the Fish Vaccines Market in India

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