Recombinant Adenovirus Vector Infection of Human Dendritic Cells

Adams, William C.; Loré, Karin

doi:10.5772/20255

Cited by 2 publications

(2 citation statements)

References 78 publications

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“…They have a broad infectivity spectrum and transduce non-dividing cells, and their genome is non-integrative [1]. As described above, they also provoke an inflammatory response in antigen-presenting cells (APCs) that serves an adjuvant function to amplify the immune response necessary to vaccinate efficiently [2,3,142,235,236]. Moreover, Ad-derived vectors have been extensively used in preclinical and clinical trials to prove their safety [237,238].…”

Section: Adenoviruses As (Vaccine) Vectorsmentioning

confidence: 99%

“…Their high production yield and stability allow industrial production under good manufacturing practice (GMP) conditions and provide practical features (i.e., storage) for regional distribution at a reduced cost [1]. Furthermore, Ad cell delivery into antigen-presenting cells is efficient, and they naturally provoke an innate immune response that acts as an adjuvant to boost vaccination success [2,3].…”

Section: Introductionmentioning

confidence: 99%

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Understanding Post Entry Sorting of Adenovirus Capsids; A Chance to Change Vaccine Vector Properties

Daussy

Pied

Wodrich

2021

Viruses

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Adenovirus vector-based genetic vaccines have emerged as a powerful strategy against the SARS-CoV-2 health crisis. This success is not unexpected because adenoviruses combine many desirable features of a genetic vaccine. They are highly immunogenic and have a low and well characterized pathogenic profile paired with technological approachability. Ongoing efforts to improve adenovirus-vaccine vectors include the use of rare serotypes and non-human adenoviruses. In this review, we focus on the viral capsid and how the choice of genotypes influences the uptake and subsequent subcellular sorting. We describe how understanding capsid properties, such as stability during the entry process, can change the fate of the entering particles and how this translates into differences in immunity outcomes. We discuss in detail how mutating the membrane lytic capsid protein VI affects species C viruses’ post-entry sorting and briefly discuss if such approaches could have a wider implication in vaccine and/or vector development.

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Section: Adenoviruses As (Vaccine) Vectorsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Understanding Post Entry Sorting of Adenovirus Capsids; A Chance to Change Vaccine Vector Properties

Daussy

Pied

Wodrich

2021

Viruses

View full text Add to dashboard Cite

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Dendritic Cells Transfected with Adenoviral Vectors as Vaccines

Senesac

Gabrilovich

Pirruccello

et al. 2014

Methods in Molecular Biology

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Dendritic cells (DCs) are critical to the initiation of a T-cell response. They constitute the most potent antigen-presenting cell (APC) endowed with the unique capacity to stimulate an antigen-specific T-cell responses by naïve T cells. Adenoviruses (Ad) have high transduction efficiency for many cell types including cells of hematopoietic origin independent of their mitotic status, and replication-defective Ad have demonstrated a safety profile clinically. Further, Ad vectors provide a high level of transgene expression, and Ad-transduced DCs can effectively present antigenic proteins. In this chapter, we outline a functionally closed, good manufacturing protocol for the differentiation of monocytes into DCs and transduction by Ad vectors. Basic functional and phenotypic release assays are provided, as well as contrasting research approaches for Ad-transduced DC-based vaccines.

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Recombinant Adenovirus Vector Infection of Human Dendritic Cells

Cited by 2 publications

References 78 publications

Understanding Post Entry Sorting of Adenovirus Capsids; A Chance to Change Vaccine Vector Properties

Understanding Post Entry Sorting of Adenovirus Capsids; A Chance to Change Vaccine Vector Properties

Dendritic Cells Transfected with Adenoviral Vectors as Vaccines

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