Recognizing and Defining True Ras Binding Domains I: Biochemical Analysis

Wohlgemuth, Sabine; Kiel, Christina; Krämer, Astrid; Serrano, Luís; Wittinghofer, Fred; Herrmann, Christian

doi:10.1016/j.jmb.2005.02.048

Cited by 158 publications

(223 citation statements)

References 86 publications

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“…Ras can stimulate other effectors than p110a, Raf1 and RalGDS, including RIN1, AF6, NORE1, PLC-e and other RalGDS family members (RGL, RLF and RGL2) (Shao et al, 1999;Herrmann, 2003;Wohlgemuth et al, 2005). Whether these or other potential Ras effectors contribute to regulation of NF-kB signaling downstream of Ras remains to be discovered.…”

Section: Discussionmentioning

confidence: 99%

Ras effector pathways modulate scatter factor-stimulated NF-κB signaling and protection against DNA damage

et al. 2007

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Section: Discussionmentioning

confidence: 99%

Ras effector pathways modulate scatter factor-stimulated NF-κB signaling and protection against DNA damage

et al. 2007

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“…Each of the ARAPs also contains a Ras-association (RA) domain, predicted to serve as a binding site for Ras or for closely related GTPases such as Rap [53]. Rap1 is best known for its role in the regulation of both integrin-mediated and cadherinmediated adhesion [54].…”

Section: Arapsmentioning

confidence: 99%

Regulation of actin cytoskeleton dynamics by Arf-family GTPases

Myers

Casanova

2008

Trends in Cell Biology

167

139

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Small GTPases of the Arf family are best known for their role in vesicular transport, wherein they nucleate the assembly of coat proteins at sites of carrier vesicle formation. However, accumulating evidence indicates that the Arfs are also important regulators of actin cytoskeleton dynamics and are involved in a variety of actin-based processes, including cell adhesion, migration and neurite outgrowth. The mechanisms of this regulation are remarkably diverse, ranging from the integration of vesicular transport with cytoskeleton assembly to the direct regulation of Rho-family GTPase function. Here, we review recent progress in our understanding of how Arfs and their interacting proteins function to integrate membrane and cytoskeletal dynamics.

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“…A novel model to investigate the role of BAT in healthful aging and lifespan is the mouse model of the disruption of the regulator for G protein signaling 14 (RGS14), which has increased BAT. RGS14 is a complex RGS family member that contains a canonical RGS domain, a tandem (R1 and R2) Ras/Rap binding domain (Kiel et al., 2005; Wohlgemuth et al., 2005), as well as a GoLoco/GPR motif. Most prior work on RGS14 focused on its effects on embryonic development and on the visual cortex and central nervous system (Evans, Lee, Smith & Hepler, 2014; Lee et al., 2010; Lopez‐Aranda et al., 2009; Shu, Ramineni & Hepler, 2010; Vellano, Brown, Blumer & Hepler, 2013).…”

Section: Introductionmentioning

confidence: 99%

Enhanced longevity and metabolism by brown adipose tissue with disruption of the regulator of G protein signaling 14

et al. 2018

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SummaryDisruption of the regulator for G protein signaling 14 (RGS14) knockout (KO) in mice extends their lifespan and has multiple beneficial effects related to healthful aging, that is, protection from obesity, as reflected by reduced white adipose tissue, protection against cold exposure, and improved metabolism. The observed beneficial effects were mediated by improved mitochondrial function. But most importantly, the main mechanism responsible for the salutary properties of the RGS14 KO involved an increase in brown adipose tissue (BAT), which was confirmed by surgical BAT removal and transplantation to wild‐type (WT) mice, a surgical simulation of a molecular knockout. This technique reversed the phenotype of the RGS14 KO and WT, resulting in loss of the improved metabolism and protection against cold exposure in RGS14 KO and conferring this protection to the WT BAT recipients. Another mechanism mediating the salutary features in the RGS14 KO was increased SIRT3. This mechanism was confirmed in the RGS14 X SIRT3 double KO, which no longer demonstrated improved metabolism and protection against cold exposure. Loss of function of the Caenorhabditis elegans RGS‐14 homolog confirmed the evolutionary conservation of this mechanism. Thus, disruption of RGS14 is a model of healthful aging, as it not only enhances lifespan, but also protects against obesity and cold exposure and improves metabolism with a key mechanism of increased BAT, which, when removed, eliminates the features of healthful aging.

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Recognizing and Defining True Ras Binding Domains I: Biochemical Analysis

Cited by 158 publications

References 86 publications

Ras effector pathways modulate scatter factor-stimulated NF-κB signaling and protection against DNA damage

Ras effector pathways modulate scatter factor-stimulated NF-κB signaling and protection against DNA damage

Regulation of actin cytoskeleton dynamics by Arf-family GTPases

Enhanced longevity and metabolism by brown adipose tissue with disruption of the regulator of G protein signaling 14

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