Recognition of Transmembrane Protein 39A as a Tumor-Specific Marker in Brain Tumor

Park, Jisoo; Lee, Hyunji; Tran, Quangdon; Mun, Kisun; Kim, Do-Hoon; Hong, Youngeun; Kwon, So Hee; Brazil, Derek P.; Park, Jongsun; Kim, Seon Hwan

doi:10.5487/tr.2017.33.1.063

Cited by 13 publications

(13 citation statements)

References 24 publications

(26 reference statements)

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“…Western blotting was performed as previously described [16], [17]. Anticollagen (Abcam, Cambridge, UK), anti-dynamin-related protein 1 (DRP1), and anti-GTPase optic atrophy 1 (OPA1) antibodies were purchased from BD Biosciences (San Jose, CA, USA).…”

Section: Methodsmentioning

confidence: 99%

A new role for the ginsenoside RG3 in antiaging via mitochondria function in ultraviolet-irradiated human dermal fibroblasts

Lee

Hong

Tran

et al. 2019

Journal of Ginseng Research

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Background The efficacy of ginseng, the representative product of Korea, and its chemical effects have been well investigated. The ginsenoside RG3 has been reported to exhibit apoptotic, anticancer, and antidepressant-like effects. Methods In this report, the putative effect of RG3 on several cellular function including cell survival, differentiation, development and aging process were evaluated by monitoring each specific marker. Also, mitochondrial morphology and function were investigated in ultraviolet (UV)-irradiated normal human dermal fibroblast cells. Results RG3 treatment increased the expression of extracellular matrix proteins, growth-associated immediate-early genes, and cell proliferation genes in UV-irradiated normal human dermal fibroblast cells. And, RG3 also resulted in enhanced expression of antioxidant proteins such as nuclear factor erythroid 2–related factor-2 and heme oxygenase-1. In addition, RG3 affects the morphology of UV-induced mitochondria and plays a role in protecting mitochondrial dysfunction. Conclusioin RG3 restores mitochondrial adenosine triphosphate (ATP) and membrane potential via its antioxidant effects in skin cells damaged by UV irradiation, leading to an increase in proteins linked with the extracellular matrix, cell proliferation, and antioxidant activity.

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Section: Methodsmentioning

confidence: 99%

A new role for the ginsenoside RG3 in antiaging via mitochondria function in ultraviolet-irradiated human dermal fibroblasts

Lee

Hong

Tran

et al. 2019

Journal of Ginseng Research

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“…Western blot analysis was performed as the described previously (27). Protein lysates were prepared from the skin of mice and NHDF cells.…”

Section: Methodsmentioning

confidence: 99%

1,2-Dichloropropane (1,2-DCP)-Induced Angiogenesis in Dermatitis

Jin¹,

Hong²,

Lee³

et al. 2019

ToxicolRes

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1,2-Dichloropropane (1,2-DCP) has been used as an industrial solvent and a chemical intermediate, as well as in soil fumigants. Human exposure may occur during its production and industrial use. The target organs of 1,2-DCP are the eyes, respiratory system, liver, kidneys, central nervous system, and skin. Repeated or prolonged contact may cause skin sensitization. In this study, 1,2-DCP was dissolved in corn oil at 0, 2.73, 5.75, and 8.75 mL/kg. The skin of mice treated with 1,2-DCP was investigated using western blotting, hematoxylin and eosin staining, and immunohistochemistry. 1,2-DCP was applied to the dorsal skin and both ears of C57BL/6J mice. The thickness of ears and the epidermis increased significantly following treatment, and the appearance of blood vessels was observed in the dorsal skin. Additionally, the expression of vascular endothelial growth factor, which is tightly associated with neovascularization, increased significantly. The levels of protein kinase-B (PKB), phosphorylated PKB, mammalian target of rapamycin (mTOR), and phosphorylated mTOR, all of which are key components of the phosphoinositide 3-kinase/PKB/mTOR signaling pathway, were also enhanced. Taken together, 1,2-DCP induced angiogenesis in dermatitis through the PI3K/PKB/mTOR pathway in the skin.

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“…Since the sequence and function of TMEM39 family proteins appear to be highly conserved, we next characterized the localization and protein interactors of human TMEM39A. The vertebrate TMEM39 family consists of two paralogs, TMEM39A and TMEM39B (33). The TMEM39B gene is only conserved in vertebrates, and is likely produced by the duplication of an ancestral form of TMEM39A (34).…”

Section: Evolutionarily Conserved Roles Of Tmem39 Family Proteins Formentioning

confidence: 99%

“…TMEM39A is a susceptibility locus associated with various autoimmune diseases and highly up-regulated in brain tumors (33,51). TMEM39B was recently found to interact with the SARS-CoV-2 ORF9C protein, which localizes to ER-derived vesicles (52,53).…”

Section: The Collagen Secretion Phenotype Of Tmem-39 Is Independent Omentioning

confidence: 99%

The conserved ER-transmembrane protein TMEM39 coordinates with COPII to promote collagen secretion and prevent ER stress

Zhang

Luo

Barbosa

et al. 2020

Preprint

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Dysregulation of collagen production and secretion contributes to aging and tissue fibrosis of major organs. How premature collagen proteins in the endoplasmic reticulum (ER) route in specialized vesicles for secretion remains to be fully elucidated. Here, we report that TMEM39, an ER-localized transmembrane protein, regulates production and secretory cargo trafficking of procollagen. We identify the C. elegans orthologue TMEM-39 from an unbiased RNAi screen and show that deficiency of tmem-39 leads to striking defects in cuticle collagen production and constitutively high ER stress response. RNAi knockdown of the tmem-39 orthologue in Drosophila causes similar defects in collagen secretion in fat body cells. The cytosolic domain of the human orthologue TMEM39A binds to Sec23A, a vesicle coat protein that drives collagen secretion and vesicular trafficking. We propose that roles of TMEM39 in collagen secretion and preventing ER stress are likely evolutionarily conserved.

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Recognition of Transmembrane Protein 39A as a Tumor-Specific Marker in Brain Tumor

Cited by 13 publications

References 24 publications

A new role for the ginsenoside RG3 in antiaging via mitochondria function in ultraviolet-irradiated human dermal fibroblasts

A new role for the ginsenoside RG3 in antiaging via mitochondria function in ultraviolet-irradiated human dermal fibroblasts

1,2-Dichloropropane (1,2-DCP)-Induced Angiogenesis in Dermatitis

The conserved ER-transmembrane protein TMEM39 coordinates with COPII to promote collagen secretion and prevent ER stress

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