Recognition of specific patterns of amino acid inhibition of growth in higher plants, uncomplicated by glutamine-reversible `general amino acid inhibition'

Bonner, Carol A.; Jensen, Roy A.

doi:10.1016/s0168-9452(97)00213-6

Cited by 39 publications

(38 citation statements)

References 36 publications

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“…g-Aminobutyrate (GABA) also could not restore root elongation (see below), and the Pro-related amino acids Glu, Gln, Arg, and Orn were strongly inhibitory to root elongation. Inhibition of plant growth by exogenous amino acids has been observed in a number of studies (Bonner and Jensen, 1997;Chen et al, 2010), and this is likely related to feedback inhibition or disturbance of metabolic equilibrium caused by applied amino acids or disruption of metabolite transport. Such observations also illustrate the uniqueness of Pro in promoting growth at low c w .…”

Section: Pro Is Required For Aba Protection Of Root Elongation and Stmentioning

confidence: 99%

Essential Role of Tissue-Specific Proline Synthesis and Catabolism in Growth and Redox Balance at Low Water Potential

2011

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To better define the still unclear role of proline (Pro) metabolism in drought resistance, we analyzed Arabidopsis (Arabidopsis thaliana) Ɗ1-pyrroline-5-carboxylate synthetase1 (p5cs1) mutants deficient in stress-induced Pro synthesis as well as proline dehydrogenase (pdh1) mutants blocked in Pro catabolism and found that both Pro synthesis and catabolism were required for optimal growth at low water potential (ψw). The abscisic acid (ABA)-deficient mutant aba2-1 had similar reduction in root elongation as p5cs1 and p5cs1/aba2-1 double mutants. However, the reduced growth of aba2-1 but not p5cs1/aba2-1 could be complemented by exogenous ABA, indicating that Pro metabolism was required for ABA-mediated growth protection at low ψw. PDH1 maintained high expression in the root apex and shoot meristem at low ψw rather than being repressed, as in the bulk of the shoot tissue. This, plus a reduced oxygen consumption and buildup of Pro in the root apex of pdh1-2, indicated that active Pro catabolism was needed to sustain growth at low ψw. Conversely, P5CS1 expression was most highly induced in shoot tissue. Both p5cs1-4 and pdh1-2 had a more reduced NADP/NADPH ratio than the wild type at low ψw. These results indicate a new model of Pro metabolism at low ψw whereby Pro synthesis in the photosynthetic tissue regenerates NADP while Pro catabolism in meristematic and expanding cells is needed to sustain growth. Tissue-specific differences in Pro metabolism and function in maintaining a favorable NADP/NADPH ratio are relevant to understanding metabolic adaptations to drought and efforts to enhance drought resistance.

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Section: Pro Is Required For Aba Protection Of Root Elongation and Stmentioning

confidence: 99%

Essential Role of Tissue-Specific Proline Synthesis and Catabolism in Growth and Redox Balance at Low Water Potential

2011

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“…BABA alteration of the amino acid balance suggests that BABA provokes a stress response in Arabidopsis by activating the general amino acid inhibition response. This is further supported by the fact that L-glutamine, an amino acid that prevents the general amino acid stress response 18,19 can restore all tested BABA-induced phenotypes.…”

Section: Beta-aminobutyric Acid Priming By Stress Imprintingmentioning

confidence: 71%

“…14,18,19 To investigate this hypothesis further, we analyzed the free amino acid content of Arabidopsis pretreated with BABA for two days. In both experimental replicates, BABA-treated Arabidopsis accumulated more Proline, Asparagine and L-Serine than the watertreated control (Fig.…”

Section: Baba Affects the Amino Acids Balance In Arabidopsismentioning

confidence: 99%

“…Alteration of the plant amino acid balance by BABA may induce a general amino acid stress inhibition and cause the observed SIMR. 14,18,19 Proline accumulation is a common physiological response in plants facing a wide range of biotic and abiotic stresses. [20][21][22][23] Asparagine content increases in plants growing in unfavorable growth condition, such as heavy metals, soil salinity or nutrient deficiency.…”

Section: Baba Affects the Amino Acids Balance In Arabidopsismentioning

confidence: 99%

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β-aminobutyric acid priming by stress imprinting

Singh

Zimmerli

2010

Plant Signaling & Behavior

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“…The use of amino acid analogs as experimental tools for biochemical-genetic studies in higher plants is also complicated by general amino acid inhibition. Conditions were demonstrated under which pfluorophenylalanine and rn-fluorotyrosine could be used as specific antimetabolites of Lphenylalanine and L-tyrosine biosynthesis without interference fiom general amino acid inhibition (2).…”

Section: Gene-enzyme Relationships Of Aromatic Amino Acid Biosynthesimentioning

confidence: 99%

Gene-Enzyme Relationships of Aromatic Amino Acid Biosynthesis in Higher Plants

None¹

2002

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Recognition of specific patterns of amino acid inhibition of growth in higher plants, uncomplicated by glutamine-reversible `general amino acid inhibition'

Cited by 39 publications

References 36 publications

Essential Role of Tissue-Specific Proline Synthesis and Catabolism in Growth and Redox Balance at Low Water Potential

Essential Role of Tissue-Specific Proline Synthesis and Catabolism in Growth and Redox Balance at Low Water Potential

β-aminobutyric acid priming by stress imprinting

Gene-Enzyme Relationships of Aromatic Amino Acid Biosynthesis in Higher Plants

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