Recognition of six additional cystoviruses: Pseudomonas virus phi6 is no longer the sole species of the family Cystoviridae

Mäntynen, Sari; Sundberg, Lotta‐Riina; Poranen, Minna M.

doi:10.1007/s00705-017-3679-4

Cited by 34 publications

(35 citation statements)

References 52 publications

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“…dsRNA phages are a unique group of phages, which are classified in the Cystoviridae family (Mantynen et al, 2018). Currently, there are only 7 sequenced dsRNA phages, and phiYY is the only one that infects human pathogen (Yang et al, 2016), while other 6 dsRNA phages all infects Pseudomonas syringae.…”

Section: Discussionmentioning

confidence: 99%

Development of a Bacteriophage Cocktail to Constrain the Emergence of Phage-Resistant Pseudomonas aeruginosa

Shen²,

et al. 2020

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With the emergence of multidrug-resistant and extensively drug-resistant bacterial pathogens, phage therapy and other alternative or additional therapeutic modalities are receiving resurgent attention. One of the major obstacles in developing effective phage therapies is the evolution of phage resistance in the bacterial host. When Pseudomonas aeruginosa was infected with a phage that uses O-antigen as receptor, phage resistances typically achieved through changing or loss of O-antigen structure. In this study, we showed that dsRNA phage phiYY uses core lipopolysaccharide as receptor and therefore efficiently kills the O-antigen deletion mutants. Furthermore, by phage training, we obtained PaoP5-m1, a derivative of dsDNA phage PaoP5, which is able to infect mutants with truncated O-antigen. We then generated a cocktail by mixing phiYY and PaoP5-m1 with additional three wide host range P. aeruginosa phages. The phage cocktail was effective against a diverse selection of clinical isolates of P. aeruginosa, and in the short-term constrained the appearance of the phage-resistant mutants that had beleaguered the effectiveness of single phage. Resistance to the 5phage cocktail emerges after several days, and requires mutations in both wzy and migA Thus, this study provides an alternative strategy for designing phage cocktail and phage therapy.

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Section: Discussionmentioning

confidence: 99%

Development of a Bacteriophage Cocktail to Constrain the Emergence of Phage-Resistant Pseudomonas aeruginosa

Shen²,

et al. 2020

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“…Two families of RNA viruses are known to infect bacteria, the Cystoviridae which infect Pseudomonas sp. (48,49) and the Leviviridae, which infect Gammaproteobacteria and Alphaproteobacteria (50)(51)(52). We used a marker gene approach to find the RdRps of these RNA phage in the assembled transcripts.…”

Section: Rna Phage Potential Hosts and Ecologymentioning

confidence: 99%

Metatranscriptomic reconstruction reveals RNA viruses with the potential to shape carbon cycling in soil

Starr

Nuccio

Pett‐Ridge

et al. 2019

Preprint

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Viruses impact nearly all organisms on Earth, with ripples of influence in agriculture, health and biogeochemical processes. However, very little is known about RNA viruses in an environmental context, and even less is known about their diversity and ecology in the most complex microbial system, soil. Here, we assembled 48 individual metatranscriptomes from four habitats within a soil sampled over a 22-day time series: rhizosphere alone, detritosphere alone, a combination of the two, and unamended soil (four time points and three biological replicates per time point). We resolved the RNA viral community, uncovering a high diversity of viral sequences. We also investigated possible host organisms by analyzing metatranscriptome marker gene content. Based on viral phylogeny, much of the diversity was Narnaviridae that parasitize fungi or Leviviridae that infect Proteobacteria. Both host and viral communities appear to be highly dynamic, and rapidly diverged depending on experimental conditions. The viral communities were structured based on the presence of litter, while putative hosts appeared to be impacted by both the presence of litter and roots. A clear time signature from Leviviridae and their hosts indicated that viruses were replicating. With this time-resolved analysis, we show that RNA viruses are diverse, abundant and active in soil. Their replication causes host cell death, mobilizing carbon in a process that represents a largely overlooked component of carbon cycling in soil.

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“…It is this multimeric spike protein, P3, which enables the recognition of the host bacteria receptor pilin, the protein monomer making up bacterial pili, by the phi6 phage. The P3 protein of phages phi8, phi12, phi13, and phiYY have been suggested to be a single polypeptide or a multimer [ 45 ]. The P3 protein of phi6 adsorbs to host type IV pili of its target, Pseudomonas syringae , which then retracts to bring the phage into close proximity of the host membrane [ 46 , 47 ].…”

Section: Cystoviridaementioning

confidence: 99%

RNA Phage Biology in a Metagenomic Era

Callanan

Stockdale

Shkoporov

et al. 2018

Viruses

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The number of novel bacteriophage sequences has expanded significantly as a result of many metagenomic studies of phage populations in diverse environments. Most of these novel sequences bear little or no homology to existing databases (referred to as the “viral dark matter”). Also, these sequences are primarily derived from DNA-encoded bacteriophages (phages) with few RNA phages included. Despite the rapid advancements in high-throughput sequencing, few studies enrich for RNA viruses, i.e., target viral rather than cellular fraction and/or RNA rather than DNA via a reverse transcriptase step, in an attempt to capture the RNA viruses present in a microbial communities. It is timely to compile existing and relevant information about RNA phages to provide an insight into many of their important biological features, which should aid in sequence-based discovery and in their subsequent annotation. Without comprehensive studies, the biological significance of RNA phages has been largely ignored. Future bacteriophage studies should be adapted to ensure they are properly represented in phageomic studies.

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Recognition of six additional cystoviruses: Pseudomonas virus phi6 is no longer the sole species of the family Cystoviridae

Cited by 34 publications

References 52 publications

Development of a Bacteriophage Cocktail to Constrain the Emergence of Phage-Resistant Pseudomonas aeruginosa

Development of a Bacteriophage Cocktail to Constrain the Emergence of Phage-Resistant Pseudomonas aeruginosa

Metatranscriptomic reconstruction reveals RNA viruses with the potential to shape carbon cycling in soil

RNA Phage Biology in a Metagenomic Era

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