Recognition of (Sesc) for Easy Identification of Staphylococcus Epidermidis and Molecular and Phenotypic Study of Β-Lactam Resistance in Staphylococcus Epidermidis Isolates in Isfahan

Abstract

Background: Not only is it crucial to rapidly detect Staphylococcus epidermidis (S. epidermidis) isolates from a broad range of bacteria, but recognizing resistance agents can greatly improve current diagnostic and therapeutic strategies. Methods: The current cross-sectional study investigated 120 clinical isolates from a nosocomial S. epidermidis infection. The isolates were identified using common biochemical tests, and specific S. epidermidis surface protein C (SesC) primers were used to confirm the presence of S. epidermidis. PCR and special primers were used to detect the β-lactamase gene (blaZ). Methicillin resistance was measured using the agar screening method and antibiotic susceptibility was measured by disk diffusion. Results: 100 samples were characterized as S. epidermidis using a phenotypic and genotypic methods. From the 100 specimens examined, 80% contained blaZ. According to agar screening, 60% of isolates were methicillin-resistant. S. epidermidis isolates demonstrated the highest resistance to penicillin (93%) and the highest sensitivity to cefazolin (39%). Conclusions:The increased resistance to β-lactam antibiotics in S. epidermidis isolates is alarming, and certain precautions should be taken by healthcare systems to continuously monitor the antimicrobial pattern of S. epidermidis, so that an appropriate drug treatment can be established.

A five-year systematic review and meta-analysis study on methicillin resistant Staphylococcus epidermidis strains in Iran

“…Currently, the prevalence of S. epidermidis in Iran is reported to be about 27.8%–58.8% according to the sample type, sampling methods, diagnosis methods, and study objectives ( 12 , 14 , 21 ), and until recent years, various studies have reported an increase in resistance to methicillin in this species. The current systematic review and meta-analysis also reports the decline of MRSE infection frequency in Iran.…”

Prevalence of Virulence Genes and Antibiotic Resistance Pattern in Enterococcus Faecalis Isolated from Urinary Tract Infection in Shahrekord, Iran

“…Enterococci can grow in environments with high salt concentrations and temperatures of 10 to 45 °C. Several reports have documented that Staphylococcus epidermidis and Staphylococcus aureus and the two most important species enterococcus are among the leading causes of opportunistic human infections, 51 including urinary tract infections, infections of the surgical site and burn wound infections, bacteremia and sepsis, endocarditis, and others (1)(2)(3)(4)(5)(6)(7)(8). The microorganism first appeared in Europe in 1986 and then spread to the United States in 1998, with a 20-fold increase in nosocomial infections between 1989 and 1998 (9)(10)(11).…”