Recognition of Hydrocarbon Microseepage Using Microbial and Adsorbed Soil Gas Indicators in the Petroliferous Region of Krishna-Godavari Basin, India

Rasheed, Mohammed Abdul; Lakshmi, M.; Kalpana, M.S.; Patil, D. J.; Dayal, A. M.

doi:10.18520/cs/v112/i03/560-568

Cited by 8 publications

(9 citation statements)

References 10 publications

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“…As soon as the samples are brought to the surface, they are stored at 2 to 4 °C and stored in cryogenic conditions. Isolation and enumeration of methane-oxidizing bacteria and ethane/propane (C2/C3) oxidizing bacteria for each sample is carried out by standard plate count (SPC) method 8 . We created microbial concentration contour maps and identified the anomalous zones (Fig.…”

Section: Methodsmentioning

confidence: 99%

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Macro-seepage based potential new hydrocarbon prospects in Assam-Arakan Basin, India

Boruah

Verma

Rasheed

et al. 2022

Sci Rep

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Active macro seepages of methane that occur in between the north bank of the Brahmaputra river and Himalayan foothill region of Assam Arakan Basin, India, indicate the presence of hydrocarbon accumulation in the subsurface, but the hydrocarbon prospects in this region are not well studied. We carried out an extensive field sampling, which included a total of 58 sediment core collections from an active gas seepage location and nearby areas at a depth of 2–2.5 m. Our sample locations are placed at 1 km intervals laterally. We performed laboratory investigations and mapped near-surface chemical alterations associated with active macro seepages and microseepages. The analysis of geochemical composition of hydrocarbon gases in the sediment indicates both the biogenic and thermogenic origins of seeped hydrocarbons. The stable isotope analysis of methane suggests the presence of thermogenic as well as mixed biogenic-thermogenic gases. The presence of such mixing of gases is caused by the secondary alteration processes during their migration through potential faults and fractures. The trace elements of the sediments show anomalous concentrations at different parts of the study area, with a wide range of concentrations for Ba (54 to 492 ppm), Cu (1–25 ppm), Cr (61–329 ppm), Ni (1–42 ppm), Pb (2–48 ppm), Th (2–32 ppm), U (4–39 ppm), V (19–133 ppm) and U (0.87–6.5 ppm). There are higher concentrations of adsorbed gases, trace elements, and microbes along the identified lineaments. Such higher concentration can be triggered by high hydrocarbon-oxidizing bacteria count, which is greater than 104 cfu/gm of soil of bacterial growth around the lineaments. We identified potential hydrocarbon prospects based on the macro and micro seepage analysis using integrated geological, geochemical and microbial techniques in the study area.

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Section: Methodsmentioning

confidence: 99%

“…The trace elements anomalies in near-surface sediments above hydrocarbon reservoirs or source rocks are very common. Generally, these trace elements are found in higher concentrations around the periphery of hydrocarbon anomalies 8 .…”

Section: Introductionmentioning

confidence: 97%

Macro-seepage based potential new hydrocarbon prospects in Assam-Arakan Basin, India

Boruah

Verma

Rasheed

et al. 2022

Sci Rep

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“…Microbial anomalies have been proven to be reliable indicators of oil and gas in the subsurface (Rasheed et al 2012a). Application of hydrocarbon-oxidizing bacteria in petroleum exploration has been considered as an effective tool (Wagner et al 2002;Miqueletto et al 2011;Rasheed et al 2015Rasheed et al , 2017Zhou et al 2020). The success of microbial prospection for oil and gas has been reported to be around 90% (Wagner et al 2002;Rasheed et al 2011).…”

Section: Introductionmentioning

confidence: 99%

“…Examples include Sacramento Basin in California, Kingfisher County in Oklahoma, and another work in Germany (Wagner et al 2002;Rasheed et al 2011;Zhou et al 2020). Rasheed et al (2008Rasheed et al ( , 2012aRasheed et al ( , b, c, 2017 collected soil samples from India and determined their hydrocarbon contents by examining the hydrocarbon-oxidizing bacteria (Baum et al 2008;Rasheed et al 2012aRasheed et al , 2012bRasheed et al , 2017. The possibility of hydrocarbon exploration using microbiological method is emphasized by the fact that the hydrocarbonoxidizing bacteria exhibit some 10 3 -10 6 colony forming units per gram (cfu/g) of hydrocarbon-bearing soil/sediment depending on ecological conditions (Rasheed et al 2017).…”

Section: Introductionmentioning

confidence: 99%

Significance of petroleum seepages in hydrocarbon exploration-case study of Khourian Desert, Central Iran

Azadeh

Saberi

ZareNezhad

2021

J Petrol Explor Prod Technol

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Hydrocarbon exploration has long been based on such costly and time-intensive methods as geophysical surveys, geological studies, and drilling. In recent years, however, researchers have started to consider such inexpensive alternatives as surface geochemistry for hydrocarbon exploration. Some 100 years ago, the leakage of hydrocarbons onto the surface in the form of micro- and macro-seepages motivated researchers toward drilling a well in the Khourian Desert in the south of Semnan Province, Iran. Upon drilling the well, researchers found evidences of non-released (free) hydrocarbons. These findings drove further study of the area using surface geochemistry while considering the nearby hydrocarbon accumulation in Qom Formation. Conventional and indirect surface geochemical methods provide an insight into the relationship between surface and subsurface hydrocarbons. In the present work, the results of the Rock–Eval pyrolysis showed total organic carbon (TOC) values in the range of 0.31–4.13 wt.% and S1 peaks between 0.07 and 27.35. Sulfur isotope analysis showed a sulfur isotopic value of −0.4. The study of hydrocarbon-oxidizing bacteria showed the presence of bacterial colonies in MSM at 1.22 × 106 cfu/g of soil sample. We further investigated surface changes due to the presence of free hydrocarbons and pH variations (4.9–8) resulted from the changes in the concentrations of calcium carbonate and iron. According to the results and given the presence of organic sulfur in the samples, the occurrence of Gach-i-turush and alike phenomena was proposed in this area. The results of geo-microbial prospecting method, surface secondary changes, and sulfur isotope studies were well in agreement with the characteristics of the existing hydrocarbon reserves in this area. Surface geochemical surveys can precede other geochemical and geophysical surveys to identify surface anomalies and hence focus on more probable locales of hydrocarbon accumulation in the Khourian Desert, central Iran.

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“…These bacteria are mostly enriched due to the continuous supply of hydrocarbon gases ( Rasheed et al, 2013 ). Therefore, the anomalous distribution and activity of light hydrocarbons (C 1 –C 4 alkanes) degraders can serve as an indicator for oil and gas deposits ( Veena Prasanna et al, 2013 ; Rasheed et al, 2017 ). Although the distribution, abundance, and community composition of methanotrophs are well-studied in oil and gas fields ( Zhang et al, 2010 ; Kadnikov et al, 2012 ; Xu et al, 2013b ), the anomalously high density of BOB is considered the most promising indicator for petroleum prospecting, as butane can only come from oil and gas reservoirs ( Zhang et al, 2013 ).…”

Section: Introductionmentioning

confidence: 99%

Novel Butane-Oxidizing Bacteria and Diversity of bmoX Genes in Puguang Gas Field

Deng

Yang

et al. 2018

Front. Microbiol.

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To investigate the diversity of butane-oxidizing bacteria in soils contaminated by long-term light hydrocarbon microseepage and the influence of butane on the soil microbial community, a quantitative study and identification of butane-oxidizing bacteria (BOB) in soils at the Puguang gas field were performed by DNA-based stable isotope probing (DNA-SIP). For the first time, two phylotypes corresponding to the genera Giesbergeria and Ramlibacter were identified as being directly involved in butane oxidation, in addition to the well-known light hydrocarbon degrader Pseudomonas. Furthermore, bmoX genes were strongly labeled by 13C-butane, and their abundances in gas field soils increased by 43.14-, 17.39-, 21.74-, and 30.14-fold when incubated with butane for 6, 9, 12, and 14 days, respectively, indicating that these bmoX-harboring bacteria could use butane as the sole carbon and energy source and they play an important role in butane degradation. We also found that the addition of butane rapidly shaped the bacterial community and reduced the diversity of bmoX genes in the gas field soils. These findings improve our understanding of BOB in the gas field environment and reveal the potential for their applications in petroleum exploration and bioremediation.

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Recognition of Hydrocarbon Microseepage Using Microbial and Adsorbed Soil Gas Indicators in the Petroliferous Region of Krishna-Godavari Basin, India

Cited by 8 publications

References 10 publications

Macro-seepage based potential new hydrocarbon prospects in Assam-Arakan Basin, India

Macro-seepage based potential new hydrocarbon prospects in Assam-Arakan Basin, India

Significance of petroleum seepages in hydrocarbon exploration-case study of Khourian Desert, Central Iran

Novel Butane-Oxidizing Bacteria and Diversity of bmoX Genes in Puguang Gas Field

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