Recognition of epoxy with phage displayed peptides

Swaminathan, Swathi; Cui, Yue

doi:10.1016/j.msec.2013.02.011

Cited by 13 publications

(11 citation statements)

References 18 publications

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“…Interestingly, the pattern of internalization of E1-3 peptide doxorubicin conjugate ( 3 ) was markedly different compared to free doxorubicin ( 5 ) at 15 and 30 min post-treatment (Figure A–D). Free doxorubicin ( 5 ) is known to enter cells via passive diffusion , and hence was mainly localized to the perinuclear and nuclear regions (Figure A,C,E). In contrast, the E1-3 peptide doxorubicin conjugate ( 3 ) displayed a punctate pattern of expression throughout the cell cytoplasm along with some perinuclear and nuclear expression (Figure B–D).…”

Section: Resultsmentioning

confidence: 99%

Identification of Novel Medulloblastoma Cell-Targeting Peptides for Use in Selective Chemotherapy Drug Delivery

et al. 2019

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Medulloblastoma is a malignant brain tumor diagnosed in children. Chemotherapy has improved survival rates to approximately 70%; however, children are often left with long-term treatment side effects. New therapies that maintain a high cure rate while reducing off-target toxicity are required. We describe for the first time the use of a bacteriophage-peptide display library to identify heptapeptides that bind to medulloblastoma cells. Two heptapeptides that demonstrated high [E1-3 (1)] or low [E1-7 (2)] medulloblastoma cell binding affinity were synthesized. The potential of the peptides to deliver a therapeutic drug to medulloblastoma cells with specificity was investigated by conjugating E1-3 (1) or E1-7 (2) to doxorubicin (5). Both peptide−drug conjugates were cytotoxic to medulloblastoma cells. E1-3 doxorubicin (3) could permeabilize an in vitro blood− brain barrier and showed a marked reduction in cytotoxicity compared to free doxorubicin (5) in nontumor cells. This study provides proof-of-concept for developing peptide−drug conjugates to inhibit medulloblastoma cell growth while minimizing offtarget toxicity.

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Section: Resultsmentioning

confidence: 99%

Identification of Novel Medulloblastoma Cell-Targeting Peptides for Use in Selective Chemotherapy Drug Delivery

et al. 2019

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“…PBPs were found to specifically recognize and bind to polymer surfaces via π–π interactions, hydrophobic and electrostatic interactions, and hydrogen bonding [7,8,9,10]. PBP binding depends on the peptide conformation, the amino acid content and the chemical composition, the molecular structure, the size, and the shape of the polymer [11].…”

Section: Introductionmentioning

confidence: 99%

KnowVolution of the Polymer-Binding Peptide LCI for Improved Polypropylene Binding

et al. 2018

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The functionalization of polymer surfaces by polymer-binding peptides offers tremendous opportunities for directed immobilization of enzymes, bioactive peptides, and antigens. The application of polymer-binding peptides as adhesion promoters requires reliable and stable binding under process conditions. Molecular modes of interactions between material surfaces, peptides, and solvent are often not understood to an extent that enables (semi-) rational design of polymer-binding peptides, hindering the full exploitation of their potential. Knowledge-gaining directed evolution (KnowVolution) is an efficient protein engineering strategy that facilitates tailoring protein properties to application demands through a combination of directed evolution and computational guided protein design. A single round of KnowVolution was performed to gain molecular insights into liquid chromatography peak I peptide, 47 aa (LCI)-binding to polypropylene (PP) in the presence of the competing surfactant Triton X-100. KnowVolution yielded a total of 8 key positions (D19, S27, Y29, D31, G35, I40, E42, and D45), which govern PP-binding in the presence of Triton X-100. The recombination of two of the identified amino acid substitutions (Y29R and G35R; variant KR-2) yielded a 5.4 ± 0.5-fold stronger PP-binding peptide compared to LCI WT in the presence of Triton X-100 (1 mM). The LCI variant KR-2 shows a maximum binding capacity of 8.8 ± 0.1 pmol/cm2 on PP in the presence of Triton X-100 (up to 1 mM). The KnowVolution approach enables the development of polymer-binding peptides, which efficiently coat and functionalize PP surfaces and withstand surfactant concentrations that are commonly used, such as in household detergents.

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“…The peptides SPSTHWK and WNAKYTL were also previously reported to bind crystalline Ni3B nanoparticles [ 37 ]. Also, the peptide NERALTL appeared twice in the database, with binding activity to epoxy covered surfaces [ 38 ] and to the fusion protein of the infectious salmon anemia virus [ 39 ]. Additionally, the peptide LSNNNLR was previously reported to bind poly(dimethylsiloxane) [ 40 ].…”

Section: Resultsmentioning

confidence: 99%

Phage-Displayed Peptides Selected to Bind Envelope Glycoprotein Show Antiviral Activity against Dengue Virus Serotype 2

Guardia

Quijada

Lleonart

2017

Advances in Virology

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Dengue virus is a growing public health threat that affects hundreds of million peoples every year and leave huge economic and social damage. The virus is transmitted by mosquitoes and the incidence of the disease is increasing, among other causes, due to the geographical expansion of the vector's range and the lack of effectiveness in public health interventions in most prevalent countries. So far, no highly effective vaccine or antiviral has been developed for this virus. Here we employed phage display technology to identify peptides able to block the DENV2. A random peptide library presented in M13 phages was screened with recombinant dengue envelope and its fragment domain III. After four rounds of panning, several binding peptides were identified, synthesized, and tested against the virus. Three peptides were able to block the infectivity of the virus while not being toxic to the target cells. Blind docking simulations were done to investigate the possible mode of binding, showing that all peptides appear to bind domain III of the protein and may be mostly stabilized by hydrophobic interactions. These results are relevant to the development of novel therapeutics against this important virus.

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Recognition of epoxy with phage displayed peptides

Cited by 13 publications

References 18 publications

Identification of Novel Medulloblastoma Cell-Targeting Peptides for Use in Selective Chemotherapy Drug Delivery

Identification of Novel Medulloblastoma Cell-Targeting Peptides for Use in Selective Chemotherapy Drug Delivery

KnowVolution of the Polymer-Binding Peptide LCI for Improved Polypropylene Binding

Phage-Displayed Peptides Selected to Bind Envelope Glycoprotein Show Antiviral Activity against Dengue Virus Serotype 2

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