Recognition of conserved surface protein epitopes on Anaplasma centrale and Anaplasma marginale isolates from Israel, Kenya and the United states

Palmer, Guy H.; Barbet, Anthony F.; Musoke, A.J.; Katende, Josrph M.; Rurangirwa, Frid; Shkap, Varda; Pipano, E.; Davis, William C.; McGuire, Travis C.

doi:10.1016/0020-7519(88)90033-1

Cited by 56 publications

(43 citation statements)

References 9 publications

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“…3; Table 1) (28,45). Importantly, the Florida strain does not assemble an appendage within infected erythrocytes (1,13,28,31,34,45). To confirm this previous observation and to test whether there are differences FIG.…”

Section: Resultsmentioning

confidence: 99%

Identification of a Novel Anaplasma marginale Appendage-Associated Protein That Localizes with Actin Filaments during Intraerythrocytic Infection

Stich¹,

Olah²,

Brayton

et al. 2004

Infect Immun

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The rickettsial pathogen Anaplasma marginale assembles an actin filament bundle during intracellular infection. Unlike other bacterial pathogens that generate actin filament tails, A. marginale infects mature erythrocytes, and the F-actin appendages are assembled on the cytoplasmic surface of a vacuole containing several organisms. To identify A. marginale molecules associated with these filaments, two complementary approaches were used: matrix-assisted laser desorption ionization-time-of-flight mass spectrometry and tandem mass spectrometry of A. marginale proteins identified with an appendage-specific monoclonal antibody and expression screening of an A. marginale phage library. Amino acid and nucleotide sequences were mapped to a full-length gene in the genome of the St. Maries strain of A. marginale; the correct identification was confirmed by expression of full-length recombinant protein and its reactivity with appendage-specific antibodies. Interestingly, there is marked variation in the abilities of diverse A. marginale strains to assemble the F-actin appendages. Comparison of four strains, the Florida, Illinois, St. Maries, and Virginia strains, revealed substantial polymorphism in the gene encoding the appendage-associated protein, with amino acid sequence identity of as low as 34% among strains. However, this variation does not underlie the differences in expression, as there is no specific polymorphism associated with loss of ability to assemble actin appendages. In contrast, the ability to assemble an actin filament bundle reflected dramatic strain-specific differences in the expression level of the appendage-associated protein. Understanding how this protein influences the cycle of invasion, replication, and egress in the host cell may provide new insights into pathogen-host interactions.

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Section: Resultsmentioning

confidence: 99%

Identification of a Novel Anaplasma marginale Appendage-Associated Protein That Localizes with Actin Filaments during Intraerythrocytic Infection

Stich¹,

Olah²,

Brayton

et al. 2004

Infect Immun

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“…Although A. marginale has a global distribution, the prevalence and incidence are highest in regions where the tropical cattle fever tick Boophilus microplus is endemic (19,21,25,28). Larval, nymphal, and adult stages of this tick all preferentially feed on cattle, and each can efficiently acquire and transmit A. marginale (1,24). This high vectorial capacity of B. microplus results in most calves in subtropical and tropical regions being infected within the first year of life, and this high incidence represents a severe constraint on animal health and production (11).…”

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confidence: 99%

Transmission of Anaplasma marginale by Boophilus microplus : Retention of Vector Competence in the Absence of Vector-Pathogen Interaction

et al. 2003

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Whether arthropod vectors retain competence for transmission of infectious agents in the long-term absence of vector-pathogen interaction is unknown. We addressed this question by quantifying the vector competence of two tick vectors, with mutually exclusive tropical-versus temperate-region distributions, for genetically distinct tropical-and temperate-region strains of the cattle pathogen Anaplasma marginale. The tropical cattle tick Boophilus microplus, which has been eradicated from the continental United States for over 60 years, was able to acquire and transmit the temperate St. Maries (Idaho) strain of A. marginale. Similarly, the temperateregion tick Dermacentor andersoni efficiently acquired and transmitted the Puerto Rico strain of A. marginale. There were no significant quantitative differences in infection rate or number of organisms per tick following feeding on cattle with persistent infections of either A. marginale strain. In contrast, the significantly enhanced replication of the Puerto Rico strain in the salivary gland of B. microplus at the time of transmission feeding is consistent with adaptation of a pathogen strain to its available vector. However, the transmission of both strains by B. microplus demonstrates that adaptation or continual interaction between the pathogen and vector is not required for retention of vector competence. Importantly, the results clearly show that reestablishment of acaricide-resistant B. microplus in the United States would be associated with A. marginale transmission.

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“…There were no significant differences in the proliferative responses to the P12, A. marginale P12 18 , and A. centrale P12 18 peptides by any of the T-cell lines. In addition, each T-cell line responded similarly to A. marginale P12 19 (323 to 341 peptide), A. marginale P12 20 (322 to 341 peptide), A. centrale P12 19 peptide, and A. centrale P12 20 (data not shown), indicating that the V3I substitution found in A. centrale had no effect on the epitope at either terminal or subterminal positions. All cell lines responded to A. marginale lysate (Fig.…”

Section: Conservation Of Cd4mentioning

confidence: 91%

Expression of Major Surface Protein 2 Variants with Conserved T-Cell Epitopes in Anaplasma centrale Vaccinates

Shkap¹,

Molad²,

Brayton

et al. 2002

Infect Immun

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Major surface protein 2 (MSP-2), identified as a protection-inducing immunogen against Anaplasma marginale challenge, is an immunodominant outer membrane protein with orthologues in all examined Anaplasma species. Although immunization with live Anaplasma centrale has long been used to induce protection against acute disease upon challenge with virulent A. marginale, its MSP-2 structure and whether MSP-2 variants are generated during persistence of the vaccine strain was unknown. In this study, we showed that the A. centrale vaccine strain persisted for a minimum of 4 years postvaccination and generated sequential MSP-2 variants. ؉ -T-cell epitopes between these two genetically distinct Anaplasma species indicates that they present a similar repertoire of MSP-2 epitopes to the immune system and that this similarity may be responsible for all or part of the A. centrale vaccine efficacy.

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Recognition of conserved surface protein epitopes on Anaplasma centrale and Anaplasma marginale isolates from Israel, Kenya and the United states

Cited by 56 publications

References 9 publications

Identification of a Novel Anaplasma marginale Appendage-Associated Protein That Localizes with Actin Filaments during Intraerythrocytic Infection

Identification of a Novel Anaplasma marginale Appendage-Associated Protein That Localizes with Actin Filaments during Intraerythrocytic Infection

Transmission of Anaplasma marginale by Boophilus microplus : Retention of Vector Competence in the Absence of Vector-Pathogen Interaction

Expression of Major Surface Protein 2 Variants with Conserved T-Cell Epitopes in Anaplasma centrale Vaccinates

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