Recognition of cell surface receptors is controlled by invertible DNA of phage Mu

Kamp, Dietmar; Sandulache, Rodica

doi:10.1111/j.1574-6968.1983.tb00273.x

Cited by 17 publications

(4 citation statements)

References 17 publications

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“…Although Mu is unrelated to P1 (and to P2), the tail fiber genes of Mu are similar to those of P1 (19,44,50,91) and therefore possibly to those of P2. Further indirect evidence in support of the hypothesis that the tail fiber genes of P2 are similar to those of P1 and Mu is supplied by the following observations: (i) the receptors for these three phages are located in the core structure of the lipopolysaccharide on the outer surface of the bacterial cell (9,11,51,59,81), and (ii) bacterial strains selected for resistance to P2 are usually resistant also to P1 and Mu but not to a wide variety of other phages (6,34).…”

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confidence: 85%

DNA sequences of the tail fiber genes of bacteriophage P2: evidence for horizontal transfer of tail fiber genes among unrelated bacteriophages

1992

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We have determined the DNA sequence of the bacteriophage P2 tail genes G and H, which code for polypeptides of 175 and 669 residues, respectively. Gene H probably codes for the distal part of the P2 tail fiber, since the deduced sequence of its product contains regions similar to tail fiber proteins from phages Mu, P1, lambda, K3, and T2. The similarities of the carboxy-terminal portions of the P2, Mu, ann P1 tail fiber proteins may explain the observation that these phages in general have the same host range. The P2 H gene product is similar to the products of both lambda open reading frame (ORF) 401 (stf, side tail fiber) and its downstream ORF, ORF 314. If 1 bp is inserted near the end of ORF 401, this reading frame becomes fused with ORF 314, creating an ORF that may represent the complete stf gene that encodes a 774-amino-acid-long side tail fiber protein. Thus, a frameshift mutation seems to be present in the common laboratory strain of lambda. Gene G of P2 probably codes for a protein required for assembly of the tail fibers of the virion. The entire G gene product is very similar to the products of genes U and U' of phage Mu; a region of these proteins is also found in the tail fiber assembly proteins of phages TuIa, TuIb, T4, and lambda. The similarities in the tail fiber genes of phages of different families provide evidence that illegitimate recombination occurs at previously unappreciated levels and that phages are taking advantage of the gene pool available to them to alter their host ranges under selective pressures.

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confidence: 85%

DNA sequences of the tail fiber genes of bacteriophage P2: evidence for horizontal transfer of tail fiber genes among unrelated bacteriophages

1992

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“…The inversion is mediated by the site-specific recombination function Gin which is encoded in the neighbouring/~ region. Depending on the orientation of the G segment, genes S and U or S' and U' are expressed, producing different tail fibres which enable phage Mu to recognize different receptors on host cell surfaces (Kamp & Sandulache, 1983;Grundy & Howe, 1984). At the boundaries of the G segment inverted repeat (IR) sequences (Hsu & Davidson, 1974), which are 34 bp long, are thought to be the substrates for Gin-mediated recombination (R. Kahmann & D. Kamp, unpublished results).…”

Section: Introductionmentioning

confidence: 99%

DNA Inversion in Bacteriophage Mu: Characterization of the Inversion Site

Schmucker

Ritthaler

Stern

et al. 1986

Journal of General Virology

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SUMMARYGin-mediated site-specific recombination promotes inversion of the G segment of phage Mu. The crossover takes place between two 34 bp-long inverted repeat sequences flanking the G segment. We have characterized the inversion site, the target for the site-specific recombination mechanism. An artificial invertible segment was constructed which consists of parts of the invertible segments of Mu and phage P1, which in this respect are largely homologous, Upon inversion of this hybrid segment the crossover site could be located, by DNA sequencing, in the ACCT sequence of the centre of symmetry in the inverted repeat in Mu. The hybrid Mu-P1 segment inverts at a lower frequency than its parental invertible segments probably because of the mismatches between the inverted repeats of Mu and P 1. This suggests that base pairing between the inverted repeats is an intermediate step in recombination. Plasmids with subcloned G segments lacking the adjacent/~ region of Mu or the corresponding region in P7, a relative of P1, are deficient in inversion. By analysis through site-specific mutagenesis of Mu DNA, an enhancer element with multiple recognition sites was identified which is necessary for efficient inversion. This component of the inversion site was located in a 170 bp segment within the Mu/~ region, 30 bp to the right of the inverted repeat sequence, but can be separated from the crossover site by a 1200 bp insertion without losing its effect.

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“…Which pair is expressed depends on the orientation of the invertible G region of the phage genome (Van de Putte et al, 1980). Two phage types G( +) and G( -), recognize different cell surface receptors, which in both cases are located in the lipopolysaccharide (LPS) of the outer membrane of Gram-negative bacteria (Kamp & Sandulache, 1983). Some bacteria, such as Escherichia coli K 12 and 'Arizona hinshawii' are sensitive to Mu particles having the specificity corresponding to the G region in the + orientation, while others, such as Citrobacter freundii and Erwinia chrysanthemi are sensitive to phage particles having the specificity corresponding to the opposite (-) orientation of the G region (Van de Putte et a/., 1980).…”

Section: Introductionmentioning

confidence: 99%

Behaviour of Temperate Phage Mu in Salmonella typhi

Soberón¹,

Gama²,

Richelle³

et al. 1986

Microbiology

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We have developed a convenient system for genetic analysis of Salmonella typhi exploiting the properties of the mutator phage Mu. In spite of the fact that wild-type Salmonella typhi strains do not allow Mu to form plaques on them, we have shown that these strains are actually sensitive to the phage. It proved possible to use Mu to induce mutations and to promote intra-and interspecific genetic transfer, without having to introduce the phage into the bacteria by means other than infection. Furthermore, we isolated Salmonella typhi derivatives on which Mu formed plaques, and studied the behaviour of Mu in these and wild-type strains.

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Recognition of cell surface receptors is controlled by invertible DNA of phage Mu

Cited by 17 publications

References 17 publications

DNA sequences of the tail fiber genes of bacteriophage P2: evidence for horizontal transfer of tail fiber genes among unrelated bacteriophages

DNA sequences of the tail fiber genes of bacteriophage P2: evidence for horizontal transfer of tail fiber genes among unrelated bacteriophages

DNA Inversion in Bacteriophage Mu: Characterization of the Inversion Site

Behaviour of Temperate Phage Mu in Salmonella typhi

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