Recognition of B-DNA by Neomycin−Hoechst 33258 Conjugates

Willis, Bert; Arya, Dev P.

doi:10.1021/bi0609265

Cited by 53 publications

(53 citation statements)

References 58 publications

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“…192, 193 The AGs conjugates NEO-Hoechst 33258 have been found to have higher affinity and specificity to HIV-1 trans-activation response element (TAR) RNA. 194 AG-arginine conjugates (AArCs) and AG-polyarginine conjugates (APArCs) have also been extensively investigated as potential treatment of HIV. 195, 196 NEO and APR have also been found to bind HIV-1 dimerization initiation (DIS), which is responsible for genome initiation.…”

Section: Newer Applications Of Aminoglycosidesmentioning

confidence: 99%

A review of patents (2011–2015) towards combating resistance to and toxicity of aminoglycosides

Chandrika

Garneau‐Tsodikova

2016

Med. Chem. Commun.

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Since the discovery of the first aminoglycoside (AG), streptomycin, in 1943, these broad-spectrum antibiotics have been extensively used for the treatment of Gram-negative and Gram-positive bacterial infections. The inherent toxicity (ototoxicity and nephrotoxicity) associated with their long-term use as well as the emergence of resistant bacterial strains have limited their usage. Structural modifications of AGs by AG-modifying enzymes, reduced target affinity caused by ribosomal modification, and decrease in their cellular concentration by efflux pumps have resulted in resistance towards AGs. However, the last decade has seen a renewed interest among the scientific community for AGs as exemplified by the recent influx of scientific articles and patents on their therapeutic use. In this review, we use a non-conventional approach to put forth this renaissance on AG development/application by summarizing all patents filed on AGs from 2011–2015 and highlighting some related publications on the most recent work done on AGs to overcome resistance and improving their therapeutic use while reducing ototoxicity and nephrotoxicity. We also present work towards developing amphiphilic AGs for use as fungicides as well as that towards repurposing existing AGs for potential newer applications.

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Section: Newer Applications Of Aminoglycosidesmentioning

confidence: 99%

A review of patents (2011–2015) towards combating resistance to and toxicity of aminoglycosides

Chandrika

Garneau‐Tsodikova

2016

Med. Chem. Commun.

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“…Concentration-dependent interactions between this compound and DNA were observed in DNA solution by circular dichroism (CD) [10] and fluorescence emission spectra [11,12]. What is of great significance is that this compound seemed to interact in a sequence-specific manner with an AT-repeated sequence of double-helical DNA and this is, to the best of our knowledge, the first time a phenanthroindolizidine alkaloid was found to have sequence-specific interactions with duplex DNA.…”

Section: Introductionmentioning

confidence: 99%

Interaction Studies of an Anticancer Alkaloid, (+)-(13aS)-Deoxytylophorinine, with Calf Thymus DNA and Four Repeated Double-Helical DNAs

Liu¹,

Li³

et al. 2011

Chemotherapy

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Background: Phenanthroindolizidine alkaloids are a family of plant-derived compounds with significant antineoplastic activity. The specific biomolecular targets of these alkaloids have not yet been clearly identified. (+)-(13aS)-deoxytylophorinine is a new phenanthroindolizidine alkaloid originally extracted from the roots of Tylophora atrofolliculata and Tylophora ovata in our institute. (+)-(13aS)-deoxytylophorinine exerts both in vitro and in vivoanticancer activities. Methods: The in vivo anticancer effects and toxicity of this compound were investigated in mice, and interactions between this compound and double-helical DNA sequences were studied in detail with circular dichroic spectroscopy and fluorescence spectroscopy. Viscosity measurements were applied to check the interactive mode between this compound and DNA. Results: Potent anticancer effects were observed in vivo. Also, concentration-dependent interactions were observed and this compound seemed to interact in a sequence-specific manner with AT-repeated sequences of double-helical DNA. Such interactions were proved to be intercalating by viscosity measurements. Conclusions: Anticancer alkaloid (+)-(13aS)-deoxytylophorinine can have sequence-specific interactions with DNA in an intercalating manner.

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“…There is extensive evidence describing the ability of aminoglycosides (e.g. neomycin) to bind both duplex and triplex forms of DNA, in addition to quadruplex structures, DNA:RNA hybrids, and to some extent, single-stranded nucleic acids [1][2][3][4][5][6][7][8][9][10][11][12][13][14][15][16][17][18][19][20].…”

Section: Introductionmentioning

confidence: 99%

Molecular recognition of single‐stranded RNA: Neomycin binding to poly(A)

Gray

Kumar

et al. 2009

FEBS Letters

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Edited by Hans EklundKeywords: RNA recognition Aminoglycoside Neomycin Poly(A) DNA RNA a b s t r a c t Poly(A) is a relevant sequence in cell biology due to its importance in mRNA stability and translation initiation. Neomycin is an aminoglycoside antibiotic that is well known for its ability to target various nucleic acid structures. Here it is reported that neomycin is capable of binding tightly to a single-stranded oligonucleotide (A 30 ) with a K d in the micromolar range. CD melting experiments support complex formation and indicate a melting temperature of 47°C. The poly(A) duplex, which melts at 44°C (pH 5.5), was observed to melt at 61°C in the presence of neomycin, suggesting a strong stabilization of the duplex by the neomycin.

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Recognition of B-DNA by Neomycin−Hoechst 33258 Conjugates

Cited by 53 publications

References 58 publications

A review of patents (2011–2015) towards combating resistance to and toxicity of aminoglycosides

A review of patents (2011–2015) towards combating resistance to and toxicity of aminoglycosides

Interaction Studies of an Anticancer Alkaloid, (+)-(13aS)-Deoxytylophorinine, with Calf Thymus DNA and Four Repeated Double-Helical DNAs

Molecular recognition of single‐stranded RNA: Neomycin binding to poly(A)

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