Recognition of 29 kDa surface-associated adhesive molecule ofEntamoeba histolyticaby monoclonal antibodies

Abstract

Monoclonal antibodies have been developed and used as specific probe to locate and identify a 29‐kDa molecule of axenic Entamoeba histolytica trophozoites. Monoclonal antibody produced by clone C8 (MoAb C8) strongly agglutinated the amoebic trophozoites. THe immunofluorescence of live E. histolytica trophozoites and surface fluorescence of acetone‐fixed trophozoites by MoAb C8 indicated existence of a 29‐kDa molecule on surface‐associated plasma membrane of E. histolytica. The monoclonal antibody belonged to IgG1 isotype. The prior treatment of E. histolytica trophozoites with MoAb C8 resulted in significant (P < 0.01) reduction in adherence of amoebic trophozoites to cultured Chinese Hamster Ovary cells and significant (P < 0.01) reduction in cytotoxicity to cultured Baby Hamster Kidney cells. Pretreatment of amoebic trophozoites with MoAb C8 prior to cultivation in TPS‐1 medium resulted in significant (P < 0.01) reduction in growth of the parasite. Thus, the data suggested that the surface‐exposed 29‐kDa molecule may be one of the receptors involved in E. histolytica host cell interactions and may possibly modulate amoebic disease processes.