Recognition‐based Sensors for Cellular Imaging

Near-Infrared Ratiometric Fluorescent Probe for Detecting Endogenous Cu2+ in the Brain

“…It is not straightforward to prepare binding-based probes for Cu 2+ , where the fluorescence change results from electronic interactions between the probe and the analyte. 25 This is because Cu 2+ , with its 3d 9 electron configuration, has the potential to quench fluorescence emission due to its paramagnetic nature. 26 Moreover, Cu 2+ is classified as a borderline Lewis acid, along with the main essential transition metals, such as Zn 2+ and Fe 2+ , 27 making it difficult to sense Cu 2+ with high selectivity.…”

“…Fluorescent Cu 2+ probes are less commonly applied to biological studies compared to Cu + probes. It is not straightforward to prepare binding-based probes for Cu 2+ , where the fluorescence change results from electronic interactions between the probe and the analyte . This is because Cu 2+ , with its 3d 9 electron configuration, has the potential to quench fluorescence emission due to its paramagnetic nature .…”