Recognition and enantioselection of drugs and biochemicals using molecularly imprinted polymer technology

Molecular imprinting science and technology: a survey of the literature for the years up to and including 2003

“…By far the most significant objective for studies using MIP-based stationary phases has been to characterize the recognition characteristics of imprinted polymer systems concerned, and both zonal and frontal chromatographic procedures have been utilized to this end. Although significant volumes of work have been aimed at the development of MIP-based stationary phases for analytical or preparative chromatographic separations, as witnessed by the number of reviews devoted to this particular application area, 15,16,30,64,243,448,604,606,784,[984][985][986][987][988][989][990][991][992] we still await the use of MIP-based HPLC stationary phases in commercial applications. Nonetheless, the dramatic improvements in chromatographic performance that have been achieved through the deeper insights into the mechanisms underlying MIP synthesis and function have been of great benefit to researchers in the development of other application areas.…”

Binding cross-reactivity of Boc-phenylalanine enantiomers on molecularly imprinted polymers

“…However, the curved plots observed in this study are more likely to be symptomatic of binding site heterogeneity, in agreement with the reasoning of several previous authors. 14,20 Curved Scatchard plots cannot be treated in the same way as linear plots. Extrapolations from a curved plot do not provide a direct estimation of binding site number, neither do slopes determined at high and low levels of ligand-MIP saturation give a direct indication of affinity constants for ''high affinity'' and ''low affinity'' binding sites.…”

Molecular imprinting technology: challenges and prospects for the future

“…58 The recognition mechanism in molecularly imprinted polymers prepared by non-covalent interactions against chiral templates has been discussed in several studies. 59,60 The main factors responsible for selective rebinding of ligands to the imprinted sites have been shown to be ionic interactions and hydrogen bonding. [61][62][63] The threedimensional arrangement of these interaction points, arrested by the polymerisation step, leads to an inherent chirality of the formed sites.…”