Reclassification of variants of tumor suppressor genes based on Sanger RNA sequencing without NMD inhibition
Changhee Ha,
Ja-Hyun Jang,
Young-gon Kim
et al.
Abstract:Introduction: RNA sequence analysis can be effectively used to identify aberrant splicing, and tumor suppressor genes are adequate targets considering their loss-of-function mechanisms. Sanger sequencing is the simplest method for RNA sequence analysis; however, because of its insufficient sensitivity in cases with nonsense-mediated mRNA decay (NMD), the use of cultured specimens with NMD inhibition has been recommended, hindering its wide adoption.Method: The results of Sanger sequencing of peripheral blood R… Show more
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