Reclassification of Staphylococcus pulvereri Zakrzewska-Czerwińska et al. 1995 as a later synonym of Staphylococcus vitulinus Webster et al. 1994

Švec, Pavel; Vancanneyt, Marc; Sedláček, Ivo; Engelbeen, Katrien; Štětina, Vlastimil; Swings, Jean; Petřáš, Petr

doi:10.1099/ijs.0.63080-0

Cited by 34 publications

(15 citation statements)

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“…Our dendrogram showed that the 36 reference Staphylococcus species were correctly distinguished. S. vitulinus and S. pulvereri, which have recently been shown to be one single species (27) Fig. 2 shows protein "fingerprint" heterogeneity of clinical and environmental (salt meat originated) S. epidermidis strains.…”

Section: Resultsmentioning

confidence: 99%

Identification of a Variety of Staphylococcus Species by Matrix-Assisted Laser Desorption Ionization-Time of Flight Mass Spectrometry

et al. 2010

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Whole-cell fingerprinting by matrix-assisted laser desorption ionization-time-of-flight mass spectrometry (MALDI-TOF MS) in combination with a dedicated bioinformatic software tool (MALDI Biotyper 2.0) was used to identify 152 staphylococcal strains corresponding to 22 staphylococcal species. Spectra of the 152 isolates, previously identified at the species level using a sodA gene-based oligonucleotide array, were analyzed against the main spectra of 3,030 microorganisms. A total of 151 strains out of 152 (99.3%) were correctly identified at the species level; only one strain was identified at the genus level. The MALDI-TOF MS method revealed different clonal lineages of Staphylococcus epidermidis that were of either human or environmental origin, which suggests that the MALDI-TOF MS method could be useful in the profiling of staphylococcal strains. The topology of the dendrogram generated by the MALDI Biotyper 2.0 software from the spectra of 120 Staphylococcus reference strains (representing 36 species) was in general agreement with that inferred from the 16S rRNA gene-based analysis. Our findings indicate that the MALDI-TOF MS technology, associated with a broad-spectrum reference database, is an effective tool for the swift and reliable identification of Staphylococci.

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Section: Resultsmentioning

confidence: 99%

Identification of a Variety of Staphylococcus Species by Matrix-Assisted Laser Desorption Ionization-Time of Flight Mass Spectrometry

et al. 2010

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“…Phylogeny and classification of this genus is under active investigation and has been subject to substantial revisions (Kloos et al, 1998a,b; Svec et al, 2004; Taponen et al, 2012). The non- aureus staphylococci (NAS), previously referred to as coagulase-negative staphylococci [although some species have a variable response to the coagulase test (Dos Santos et al, 2016)], are the most frequently isolated microorganisms from the mammary gland of dairy cows and increasingly recognized as etiologic agents of intramammary infection (IMI) in cattle worldwide (Pyörälä and Taponen, 2009; Sampimon et al, 2009; Thorberg et al, 2009; De Vliegher et al, 2012).…”

Section: Introductionmentioning

confidence: 99%

Comprehensive Phylogenetic Analysis of Bovine Non-aureus Staphylococci Species Based on Whole-Genome Sequencing

et al. 2016

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Non-aureus staphylococci (NAS), a heterogeneous group of a large number of species and subspecies, are the most frequently isolated pathogens from intramammary infections in dairy cattle. Phylogenetic relationships among bovine NAS species are controversial and have mostly been determined based on single-gene trees. Herein, we analyzed phylogeny of bovine NAS species using whole-genome sequencing (WGS) of 441 distinct isolates. In addition, evolutionary relationships among bovine NAS were estimated from multilocus data of 16S rRNA, hsp60, rpoB, sodA, and tuf genes and sequences from these and numerous other single genes/proteins. All phylogenies were created with FastTree, Maximum-Likelihood, Maximum-Parsimony, and Neighbor-Joining methods. Regardless of methodology, WGS-trees clearly separated bovine NAS species into five monophyletic coherent clades. Furthermore, there were consistent interspecies relationships within clades in all WGS phylogenetic reconstructions. Except for the Maximum-Parsimony tree, multilocus data analysis similarly produced five clades. There were large variations in determining clades and interspecies relationships in single gene/protein trees, under different methods of tree constructions, highlighting limitations of using single genes for determining bovine NAS phylogeny. However, based on WGS data, we established a robust phylogeny of bovine NAS species, unaffected by method or model of evolutionary reconstructions. Therefore, it is now possible to determine associations between phylogeny and many biological traits, such as virulence, antimicrobial resistance, environmental niche, geographical distribution, and host specificity.

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“…sciuri, Staphylococcus lentus, and Staphylococcus vitulinus (12,26). Staphylococcus pulvereri was a member of the S. sciuri group until recently, when it was shown that S. pulvereri is only a synonym of S. vitulinus (originally S. vitulus) (15,23). Although they are principally associated with animals, members of the S. sciuri group may colonize humans, and it has been estimated that they may constitute 0.79 to 4.3% of the total number of coagulase-negative staphylococci isolated from clinical samples (8,20).…”

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Identification and Characterization of Clinical Isolates of Members of the Staphylococcus sciuri Group

Stepanović¹,

Dakić²,

Morrison

et al. 2005

J Clin Microbiol

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A total of 28 staphylococcal isolates from human clinical specimens belonging to the Staphylococcus sciuri group were identified and characterized. The API Staph and ID32 STAPH correctly identified S. sciuri and S. lentus but not S. vitulinus strains. Identification to the subspecies level was possible only by a PCR-based method.Members of the Staphylococcus sciuri group are widespread in nature, and they can be isolated from a variety of farm animals, pets, and wild animals, as well as from various food products of animal origin (6,9,12,14,22,26 (12,26). Staphylococcus pulvereri was a member of the S. sciuri group until recently, when it was shown that S. pulvereri is only a synonym of S. vitulinus (originally S. vitulus) (15, 23). Although they are principally associated with animals, members of the S. sciuri group may colonize humans, and it has been estimated that they may constitute 0.79 to 4.3% of the total number of coagulase-negative staphylococci isolated from clinical samples (8,20). However, they have been associated with serious infections such as endocarditis (10), peritonitis (25), septic shock (11), urinary tract infection (20), endophthalmitis (1), pelvic inflammatory disease (21), and, most frequently, wound infections (16,19). The aim of this study was to compare phenotypic (conventional, API Staph, ID32 Staph) and genotypic (PCR) methods for identification of isolates of the S. sciuri group.A total of 28 isolates belonging to the S. sciuri group, recovered from 1998 to 2003 from clinical samples at the Institute of Microbiology, School of Medicine, Belgrade, Serbia, and Regional Hospital Příbram, Příbram, Czech Republic, were analyzed (Table 1). Half of them were isolated from urine samples. Some of these strains have been reported previously (18-21) but not investigated for the characteristics presented in this study. All the isolates were previously identified by conventional methods (5,12,18,26) as S. sciuri (23 strains), S. lentus (3 strains), or S. vitulinus (2 strains).Staphylocoagulase (free coagulase) activity was determined with rabbit plasma (Torlak, Belgrade, Serbia) by using the tube method (5). Oxidase activity was determined with oxidase diagnostic tablets (Rosco, Taastrup, Denmark). Novobiocin susceptibility was determined on Mueller-Hinton agar (Oxoid Limited, Basingstoke, Hampshire, United Kingdom) with a disk containing 5 g of novobiocin (Bioanalyse, Ankara, Turkey). Strains were considered to be resistant to novobiocin if the zone of inhibition was Յ16 mm. Commercial identification kits, namely, API Staph and ID32 STAPH (bioMérieux, Marcy-l'Etoile, France), were used according to the manufacturer's instructions. All the strains were coagulase negative and oxidase positive. In addition, the disk diffusion method with the 5-g novobiocin disk confirmed that all strains were resistant to novobiocin. However, only three S. sciuri strains showed resistance to novobiocin by use of the ID32 STAPH kit. The problem with determination of resistance to novobiocin by ID32 STAPH was also ...

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Reclassification of Staphylococcus pulvereri Zakrzewska-Czerwińska et al. 1995 as a later synonym of Staphylococcus vitulinus Webster et al. 1994

Cited by 34 publications

References 11 publications

Identification of a Variety of Staphylococcus Species by Matrix-Assisted Laser Desorption Ionization-Time of Flight Mass Spectrometry

Identification of a Variety of Staphylococcus Species by Matrix-Assisted Laser Desorption Ionization-Time of Flight Mass Spectrometry

Comprehensive Phylogenetic Analysis of Bovine Non-aureus Staphylococci Species Based on Whole-Genome Sequencing

Identification and Characterization of Clinical Isolates of Members of the Staphylococcus sciuri Group

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