Reclassification of 
               
                  Rhizobium tropici
               
             type A strains as Rhizobium leucaenae sp. nov.

Ribeiro, Renan Augusto; Rogel, Marco A.; López-López, Aline; Ormeño‐Orrillo, Ernesto; Barcellos, Fernando Gomes; Martinez, Jesse; Thompson, Fabiano L.; Martı́nez-Romero, Esperanza; Hungría, Mariangela

doi:10.1099/ijs.0.032912-0

Cited by 92 publications

(42 citation statements)

References 37 publications

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“…1). Previously, R. leguminosarum and E. americanum have been described as bean-nodulating rhizobia in other regions [1,7,30,36,47]. However, our study provides the first set of clear evidence that bean-nodulating R. leguminosarum-like group, R. grahamii and E. americanum populations were native to Mexico, and that no R. etli were isolated in the acid and alkaline soils in Mexico.…”

Section: Discussionmentioning

confidence: 45%

“…It has been cultivated worldwide as a grain or vegetable crop, and forms root nodules in different regions with a wide range of symbiotic nitrogen-fixing bacteria, including the Rhizobium species R. etli, R. gallicum, R. giardinii, R. leguminosarum, R. lusitanum, R. phaseoli, R. vallis, R. leucaenae [36], R. tropici [4], R. mesoamericanum [25], R. freirei [11] and R. azibense [29], as well as Ensifer meliloti [50], Ensifer americanum [30], and Bradyrhizobium sp. [17].…”

Section: Introductionmentioning

confidence: 99%

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Microsymbionts of Phaseolus vulgaris in acid and alkaline soils of Mexico

Verástegui-Valdés

Zhang

Rivera-Orduña

et al. 2014

Systematic and Applied Microbiology

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In order to investigate bean-nodulating rhizobia in different types of soil, 41 nodule isolates from acid and alkaline soils in Mexico were characterized. Based upon the phylogenetic studies of 16S rRNA, atpD, glnII, recA, rpoB, gyrB, nifH and nodC genes, the isolates originating from acid soils were identified as the phaseoli symbiovar of the Rhizobium leguminosarum-like group and Rhizobium grahamii, whereas the isolates from alkaline soils were defined as Ensifer americanum sv. mediterranense and Rhizobium radiobacter. The isolates of “R. leguminosarum” and E. americanum harbored nodC and nifH genes, but the symbiotic genes were not detected in the four isolates of the other two species. It was the first time that “R. leguminosarum” and E. americanum have been reported as bean-nodulating bacteria in Mexico. The high similarity of symbiotic genes in the Rhizobium and Ensifer populations showed that these genes had the same origin and have diversified recently in different rhizobial species. Phenotypic characterization revealed that the “R. leguminosarum” population was more adapted to the acid and low salinity conditions, while the E. americanum population preferred alkaline conditions. The findings of this study have improved the knowledge of the diversity, geographic distribution and evolution of bean-nodulating rhizobia in Mexico.

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Section: Discussionmentioning

confidence: 45%

Section: Introductionmentioning

confidence: 99%

Microsymbionts of Phaseolus vulgaris in acid and alkaline soils of Mexico

Verástegui-Valdés

Zhang

Rivera-Orduña

et al. 2014

Systematic and Applied Microbiology

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“…giardinii and bv. phaseoli) (Amarger et al, 1997), R. leucaenae (Ribeiro et al, 2012), R. freirei (Dall'Agnol et al, 2013), and R. paranaense (Dall'Agnol et al, 2014). An important feature of the bean microsymbiont rhizobia genome is the presence of DNA repeated sequences found in the symbiotic plasmid regions, pSym containing the structural nitrogenase gene, nif, or the nodulation gene, nod (Girard et al, 1991;Segovia et al, 1993).…”

Section: Introductionmentioning

confidence: 99%

New Native Rhizobia Strains for Inoculation of Common Bean in the Brazilian Savanna

Mercante

Otsubo

Brito

2017

Rev. Bras. Ciênc. Solo

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Maximization of biological nitrogen fixation in the common bean (Phaseolus vulgaris L.) crop depends on the genetic characteristics related to the plant, the symbiotic efficiency of rhizobia, and environmental factors. The objective of this study was to evaluate the performance of rhizobia selected beforehand from Cerrado (Brazilian tropical savanna) soils in Mato Grosso do Sul. The experiments were conducted in 2007 in the municipalities of Aquidauana, Anaurilândia, Campo Grande, and Dourados, all located in the state of Mato Grosso do Sul. All procedures established followed the current recommendations of the Brazilian Ministry of Agriculture (Ministério de Agricultura, Pecuária e Abastecimento -MAPA), in accordance with the "official protocol for assessing the feasibility and agronomic efficiency of strains, and inoculant technologies linked to the process of biological nitrogen fixation in legumes". The program for selection of rhizobia for inoculation in bean plants resulted in identification of different strains with high symbiotic efficiency, competitiveness, and genetic stability, based on the Embrapa Agropecuária Oeste collection of multifunctional microorganism cultures. In previous studies, 630 isolates of Rhizobium were evaluated. They were obtained from nodules of leucaena (380) or dry beans (250) from 87 locations, including 34 municipalities in Mato Grosso do Sul. Three of them stood out from the others: CPAO 12.5 L2, CPAO 17.5 L2, and CPAO 56.4 L2. Inoculation of these strains in bean plants demonstrated economic viability and high potential for obtaining a more effective inoculant suitable for trading purposes.

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“…Other changes in the genus include the naming of Rhizobium tropici type A strain into a new species R. leucaena based on 16S rRNA gene sequences and DNA-DNA hybridization (Martínez-Romero et al 1991;Ribeiro et al 2012). Chen et al (1997) reported a new rhizobium species on the basis of 16S rRNA gene sequence, DNA-DNA hybridization and phenotypic characterization, this new species belong to the phylogenetic tree branch which included R. leguminosarum.…”

Section: Rhizobiummentioning

confidence: 96%

Root-Nodule Bacteria of Legumes Growing in Semi-Arid African Soils and Other Areas of the World

Pule‐Meulenberg¹

2014

Sustainable Development and Biodiversity

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In Africa, arid and semi-arid soils are characterized by a diverse occurrence of plants including nodulated legumes which are adapted to the prevailing and often harsh environmental conditions. Such soils also harbour a large diversity of indigenous rhizobial symbionts (comparable to legume diversity) and other bacterial endophytes that have a potential to be developed into elite strains to be used as inoculum. However, very few studies have investigated the diversity of root-nodule bacteria on African soils, the main reason being that funds for research are often lacking. Discoveries of new rhizobial species are to be expected from different soil ecologies that are likely to be inhabited by various types of microorganisms. It is concluded that the semi-arid and arid African soils have rhizobia that is possibly as equally diverse as their host legumes. Research in these areas is likely to find novel species with a great potential for use in agricultural systems.

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Reclassification of Rhizobium tropici type A strains as Rhizobium leucaenae sp. nov.

Abstract: Reclassification of Rhizobium tropici type A strains as Rhizobium leucaenae sp. nov.

Cited by 92 publications

References 37 publications

Microsymbionts of Phaseolus vulgaris in acid and alkaline soils of Mexico

Microsymbionts of Phaseolus vulgaris in acid and alkaline soils of Mexico

New Native Rhizobia Strains for Inoculation of Common Bean in the Brazilian Savanna

Root-Nodule Bacteria of Legumes Growing in Semi-Arid African Soils and Other Areas of the World

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