Reclassification of Haloactinobacterium glacieicola as Occultella glacieicola gen. nov., comb. nov., of Haloactinobacterium album as Ruania alba comb. nov, with an emended description of the genus Ruania, recognition that the genus names Haloactinobacterium and Ruania are heterotypic synonyms and description of Occultella aeris sp. nov., a halotolerant isolate from surface soil sampled at an ancient copper smelter

Abstract

In the course of screening the surface soils of ancient copper mines and smelters (East Harz, Germany) an aerobic, non-motile and halotolerant actinobacterium forming small rods or cocci was isolated. The strain designated F300T developed creamy to yellow colonies on tryptone soy agar and grew optimally at 28 °C, pH 7–8 and with 0.5–2 % (m/v) NaCl. Its peptidoglycan was of type A4α l-Lys–l-Glu (A11.54). The menaquinone profile was dominated by MK-8(II, III-H4) and contained minor amounts of MK-8(H2), MK-8(H6) and MK-9(H4). The polar lipids comprised diphosphatidylglycerol, phosphatidylglycerol, phosphatidylinositol, mono and diacylated phosphatidylinositol dimannosides, and components that were not fully characterized, including two phospholipids, two glycolipids and an uncharacterized lipid. Major whole-cell sugars were rhamnose and ribose. The fatty acid profile contained mainly iso and anteiso branched fatty acids (anteiso-C15 : 0, iso-C14 : 0) and aldehydes/dimethylacetals (i.e. not fatty acids). Sequence analysis of its genomic DNA and subsequent analysis of the data placed the isolate in the group currently defined by members of the genera Ruania and Haloactinobacterium (family Ruaniaceae , order Micrococcales ) as a sister taxon to the previously described species Haloactinobacterium glacieicola , sharing an average nucleotide identity and average amino acid identity values of 85.3 and 85.7 %, respectively. Genotypic and chemotaxonomic analyses support the view that strain F300T (=DSM 108350T=CIP 111667T) is the type strain of a new genus and new species for which the name Occultella aeris gen. nov., sp. nov. is proposed. Based on revised chemotaxonomic and additional genome based data, it is necessary to discuss and evaluate the results in the light of the classification and nomenclature of members of the family Ruaniaceae , i.e. the genera Haloactinobacterium and Ruania . Consequently, the reclassification of Haloactinobacterium glacieicola as Occultella glacieicola comb. nov. and Haloactinobacterium album as Ruania alba comb. nov., with an emended description of the genus Ruania are proposed.

Aurantimicrobium photophilum sp. nov., a non-photosynthetic bacterium adjusting its metabolism to the diurnal light cycle and reclassification of Cryobacterium mesophilum as Terrimesophilobacter mesophilus gen. nov., comb. nov.

“…The peptidoglycan structure was characterized by using a previously described approach [17] to be a variant of the B1α type with the structure {Gly} [L-Lys] d -Glu(Hyg) - l -Ala - l -Ala - l -Lys [18]. In contrast to the type strain of A.…”

Ruania rhizosphaerae sp. nov., a novel actinobacterium isolated from rhizosphere of Suaeda aralocaspica

“…in 2007 [1] and emended by Schumann et al . in 2021 [2]. Members of the genus Ruania are Gram-stain-positive, aerobic, non-motile and moderately halophilic actinobacteria [2].…”

“…in 2021 [2]. Members of the genus Ruania are Gram-stain-positive, aerobic, non-motile and moderately halophilic actinobacteria [2]. The major fatty acids are anteiso-C 15 : 0 and iso-C 15 : 0 , and the predominant menaquinone is MK-8(H 4 ) [2].…”

“…Members of the genus Ruania are Gram-stain-positive, aerobic, non-motile and moderately halophilic actinobacteria [2]. The major fatty acids are anteiso-C 15 : 0 and iso-C 15 : 0 , and the predominant menaquinone is MK-8(H 4 ) [2]. At the time of writing, the genus Ruania encompasses two species with validly published names (LPSN, https://lpsn.dsmz.de/genus/ruania).…”

“…At the time of writing, the genus Ruania encompasses two species with validly published names (LPSN, https://lpsn.dsmz.de/genus/ruania). Among of them, Ruania alba [2] was isolated from salt lake, whose basonym was Haloactinobacterium album [3]; Meanwhile, Ruania albidiflava [1] was isolated from farmland soil. During a study of the diversity of the culturable rhizosphere bacteria in the halophyte Suaeda aralocaspica , strain LNNU 22110 T was isolated and purified.…”