Reclassification of 15 Streptomyces species as synonyms of Streptomyces albogriseolus, Streptomyces althioticus, Streptomyces anthocyanicus, Streptomyces calvus, Streptomyces griseoincarnatus, Streptomyces mutabilis, Streptomyces pilosus or Streptomyces rochei

Abstract

Taxonomic relationships in eight sets of Streptomyces species, (1a) Streptomyces enissocaesilis, Streptomyces plicatus, Streptomyces rochei and Streptomyces vinaceusdrappus , (1b) Streptomyces geysiriensis, (2) Streptomyces luteus and Streptomyces mutabilis, (3) Streptomyces flavoviridis and Streptomyces pilosus, (4) Streptomyces asterosporus and Streptomyces calvus, (5) Streptomyces erythrogriseus, Streptomyces griseoincarnatus, Streptomyces labedae and Streptomyces variabilis , (6a) Streptomyces griseorubens, (6b) Streptomyces matensis, (6c) Streptomyces althioticus, (7) Streptomyces albogriseolus and Streptomyces viridodiastaticus , (8a) Streptomyces humiferus and Streptomyces violaceolatus, (8b) Streptomyces anthocyanicus, Streptomyces coelescens and Streptomyces violaceoruber, were investigated. Type strains within each subset of 1a to 8b shared completely identical 16S rRNA gene sequences. In MLSA, subsets 1a and 1b, 6a to 6c, and 8a and 8b formed an independent clade, respectively, but the evolutionary distances between S. violaceoruber and the other members in set 8 and between S. griseorubens and those in set 6 were 0.022–0.023 and 0.0064–0.0076, respectively. Members in each of the other sets, except for S. labedae , formed an independent clade. In each clade, evolutionary distances between/among the members were <0.007 except for that between S. griseorubens and S. matensis in set 6, suggesting the same species. Digital DNA–DNA relatedness using whole genome sequences and phenotypic similarities supported the synonymies of sets 1 to 3, set 4 except for S. labedae , sets 5 to 7, and set 8 except for S. violaceoruber , respectively. Therefore, S. enissocaesilis, S. geysiriensis, S. plicatus and S. vinaceusdrappus were considered as later heterotypic synonyms of S. rochei; S. luteus as that of S. mutabilis; S. flavoviridis as that of S. pilosus; S. asterosporus as that of S. calvus; S. erythrogriseus and S. variabilis as those of S. griseoincarnatus; S. griseorubens and S. matensis as that of S. althioticus; S. viridodiastaticus as that of S. albogriseolus; S. coelescens, S. humiferus and S. violaceolatus as those of S. anthocyanicus .

Differences at Species Level and in Repertoires of Secondary Metabolite Biosynthetic Gene Clusters among Streptomyces coelicolor A3(2) and Type Strains of S. coelicolor and Its Taxonomic Neighbors

“…coelicolor, S. daghestanicus, S. hydrogenans, and S. violascens also share the same 16S rDNA sequence, but these four are independent species. It is often reported that the resolution of 16S rDNA sequences is so low that we are unable to classify Streptomyces members at the species level by only analyzing 16S rDNA sequences [15,55,56]. The present study suggests that each species harbors specific smBGCs.…”

Cliona varians-Derived Actinomycetes as Bioresources of Photoprotection-Related Bioactive End-Products

“…The most bioactive isolate (i.e., CLIVUS-G1225) was related in a clade with Streptomyces viridosporus, Streptomyces geysiriensis, and Streptomyces minutiscleroticus (Figure 7). Recently, S. geysiriensis was considered as a later heterotypic synonym of Streptomyces rochei [42]. To consider the photoprotective potential shown by the actinomycetes end-products, we integrated the antioxidant and SPFi bioactivities into a radar chart (Figure 9).…”

“…The most bioactive isolate (i.e., CLIVUS-G1225) was related in a clade with Streptomyces viridosporus, Streptomyces geysiriensis, and Streptomyces minutiscleroticus (Figure 7). Recently, S. geysiriensis was considered as a later heterotypic synonym of Streptomyces rochei [42].…”

Reclassification of four subspecies in the genus Streptomyces to Streptomyces rubradiris sp. nov., Streptomyces asoensis sp. nov., Streptomyces fructofermentans sp. nov. and Streptomyces ossamyceticus sp. nov.

“…Strains showing <99.0% similarity are members of different species for which DNA–DNA hybridisation (DDH) experiments can be safely omitted without a significant risk of incorrectly differentiated species [7], although 98.7% is generally recognized as the threshold in prokaryotes [8]. In the genus Streptomyces , the threshold is suggested to be higher [9]. Recently, multilocus sequence analysis (MLSA) has been used not only to examine phylogenetic relationships but also to identify strains at the species level because of its higher resolution than 16S rRNA gene sequence analysis.…”