Reciprocal repression between Fgf8 and miR-133 regulates cardiac induction through Bmp2 signaling

Abstract

This data article contains complementary figures and results related to the research article entitled “Negative Fgf8-Bmp2 feed-back is controlled by miR-130 during early cardiac specification” [15], which reveals what specific role miR-130 plays during the cardiac induction process. This study evidenced miR-130 a putative microRNA that targets Erk1/2 (Mapk1) 3′UTR- as a necessary linkage in the control of Fgf8 signaling, mediated by Bmp2. Thus, miR-130 regulates a negative Fgf8-Bmp2 feed-back loop responsible to achieve early cardiac specification. A significant aspect supporting our conclusions is given by the expression pattern of miR-130 during early cardiac specification, as well as by those results obtained after the designed experimental procedures. The data presented here reveal that miR-133 is also expressed within the precardiac areas during early cardiogenesis, pattern which is comparable to that of FGFR1, receptor involved in the Fgf8/ERK signaling pathway. Interestingly, our miR-133 overexpression experiments resulted in a decrease of Fgf8 expression, whereas we observed an increase of Bmp2 and subsequently of cardiac specific markers Nkx-2.5 and Gata4. Additionally, our loss-of-function experiments -through Fgf8 siRNA electroporation- showed an increase of miR-133 expression. Finally, after our Bmp2 experiments, we observed that miR-133 is upstream-regulated by Bmp2. All those results suggest that miR-133 also constitutes a crucial linkage in the crosstalk between Fgf8 and Bmp2 signaling by regulating the Fgf8/ERK pathway during cardiac induction.

Identification of the Genetic Basis for the Large-tailed Phenotypic Trait in Han Sheep Through Integrated mRNA and miRNA Analysis of Tail Fat Tissue Samples

“…Smemo et al (2014) established IRX3 as a functional longrange target of obesity-associated FTO variants and a key regulator of overall body composition [73]. Lopez-Sanchez et al (2015) also determined that miR-133 is an essential mediator of crosstalk between the Fgf8 and Bmp2 signaling pathway owing to its ability to regulate Fgf8/ERK signaling in the context of cardiac induction [74], in line with our identi ition of Fgf8 as a miR-133 target genes. IGF2 is a DEM target gene that has likely been subjected to recent selective pressure, given that most domestic European pigs selected for lean growth carry a particular IGF2 allele that induces decreased fat deposition and increased muscle growth [75].…”

DNA methylation and regulation of gene expression: Guardian of our health

“…Significant changes in the ratio of vitamin intake were found to affect the CpG island methylation pattern in the loci of DNMT1, DNMT3A and DNMT3B, consequently leading to increased expression of the methyltransferases [ 29 ]. Even levels of miRNAs like that of miR-133 (linked to muscle and cardiac tissue metabolism and certain kinds of cancer) [ 54 , 58 , 65 , 85 , 86 , 98 , 101 ] and miR-221 (linked to oncogenesis) [ 10 , 25 , 50 , 56 , 60 , 71 , 79 , 90 , 96 , 100 ] changed in response to a skewed ratio of intake of the two vitamins. Other than sub or supra-optimal levels of folate or B12 intake, vitamin C or ascorbic acid consumption during the perinatal period also seems significant [ 19 ].…”

MAP/ERK Signaling in Developing Cognitive and Emotional Function and Its Effect on Pathological and Neurodegenerative Processes

“…ERK participates in two stages of brain development that can be conceptualized as embryonic and early postnatal development. In the embryonic period, FGF8 signaling along ERK participates in proliferation, migration, and the final fate of neural cells, which results in the configuration of the main brain subdivisions [ 24 , 25 , 26 , 27 , 28 , 29 , 30 ]. In postnatal development, MAPK/ERK signaling configures the specific networks for the correct processing of emotional signals.…”