Reciprocal Interaction between Vascular Filopodia and Neural Stem Cells Shapes Neurogenesis in the Ventral Telencephalon

Marco, Barbara Di; Crouch, Elizabeth E.; Shah, Bhavin; Duman, Ceren; Paredes, Mercedes F.; Almodóvar, Carmen Ruiz de; Huang, Eric J.; Alfonso, Julieta

doi:10.1016/j.celrep.2020.108256

Cited by 32 publications

(41 citation statements)

References 57 publications

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“…At this time point, we noticed that endocardial protrusions can be detected in close proximity to trabecular CMs (Figure 1D’’ and D’’’, Figure 1-video 3). Recently, it has been shown that endothelial protrusions modulate neurogenesis by affecting progenitor proliferation in the developing brain (Di Marco et al, 2020). To determine whether endocardial protrusions affect CM proliferation, we performed EdU labeling between 28 and 72 hpf and analyzed the heart at 72 hpf.…”

Section: Resultsmentioning

confidence: 99%

Apelin signaling dependent endocardial protrusions promote cardiac trabeculation in zebrafish

Rittershaus

Priya

et al. 2021

Preprint

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During cardiac development, endocardial cells (EdCs) produce growth factors to promote myocardial morphogenesis and growth. In particular, EdCs produce Neuregulin which is required for ventricular cardiomyocytes (CMs) to seed the multicellular ridges known as trabeculae. Defects in Neuregulin signaling, or in endocardial sprouting towards CMs, cause hypotrabeculation. However, the mechanisms underlying endocardial sprouting remain largely unknown. Here, we first show by live imaging in zebrafish embryos that EdCs interact with CMs via dynamic membrane protrusions. After touching CMs, these protrusions remain in close contact with their target despite the vigorous cardiac contractions. Loss of the CM-derived peptide Apelin, or of the Apelin receptor, which is expressed in EdCs, leads to reduced endocardial sprouting and hypotrabeculation. Mechanistically, Neuregulin signaling requires endocardial protrusions to activate extracellular signal-regulated kinase (Erk) signaling in CMs and trigger their delamination. Altogether, these data show that Apelin signaling dependent endocardial protrusions modulate CM behavior during trabeculation.

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Section: Resultsmentioning

confidence: 99%

Apelin signaling dependent endocardial protrusions promote cardiac trabeculation in zebrafish

Rittershaus

Priya

et al. 2021

Preprint

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“…Neurogenesis and angiogenesis are two different processes that seem to be tightly coupled during embryonic neural development. Nascent blood vessels were reported to actively contact dividing neural stem cells and have a function in their behavior, impacting proper brain development (Di Marco et al, 2020). To further understand how culturing cerebellar organoids in dynamic conditions impacts on angiogenic transcriptional profiles, we analyzed the number of normalized read counts of representative genes annotated in the sprouting angiogenesis process (GO:0002040).…”

Section: Transcriptional Modulation Of Genes Involved In Angiogenic Processes By Dynamic Conditionmentioning

confidence: 99%

Transcriptome profiling of human pluripotent stem cell‐derived cerebellar organoids reveals faster commitment under dynamic conditions

Silva

Sousa-Luís

Fernandes

et al. 2021

Biotech & Bioengineering

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“…The vWF-A domain 1 directly binds to angiogenesis-inducing factors such as vascular endothelial growth factor-A (VEGF-A) and platelet-derived growth factor-BB (PDGF-BB). VEGF and PDGF-BB play an important role in vascularization [23] and neurogenesis, respectively, during early development [24].…”

Section: Possible Roles Of the Vwf-a And Lccl Domains In Akhmentioning

confidence: 99%

“…Although the interaction of blood vessels with NSCs in the adult brain niche has already been widely reported [25][26][27], recent reports have shown that the timing of differentiation of NSCs/NPCs into neurons is implicated in the interaction between embryonic vascularization and neurogenesis. For instance, Di Marco et al showed that nascent periventricular vessels interact with dividing apical neural progenitors by using vascular filopodia induced by the upregulation of VEGF-A in a cell-cycle-dependent manner [23]. They concluded that vascular filopodia helps in fine-tuning NSC behavior for proper brain development.…”

Section: Possible Roles Of the Vwf-a And Lccl Domains In Akhmentioning

confidence: 99%

Regulation of the Brain Neural Niche by Soluble Molecule Akhirin

Kudo

Ohta

2021

JDB

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In the central nervous system (CNS), which comprises the eyes, spinal cord, and brain, neural cells are produced by the repeated division of neural stem cells (NSCs) during the development of the CNS. Contrary to the notion that the CNS is relatively static with a limited cell turnover, cells with stem cell-like properties have been isolated from most neural tissues. The microenvironment, also known as the NSC niche, consists of NSCs/neural progenitor cells, other neurons, glial cells, and blood vessels; this niche is thought to regulate neurogenesis and the differentiation of NSCs into neurons and glia. Although it has been established that neurons, glia, and blood vessels interact with each other in a complex manner to generate neural tissues in the NSC niche, the underlying molecular mechanisms in the CNS niche are unclear. Herein, we would like to introduce the extracellular secreted protein, Akhirin (AKH; Akhi is the Bengali translation for eye). AKH is specifically expressed in the CNS niche—the ciliary body epithelium in the retina, the central canal of the spinal cord, the subventricular zone, and the subgranular zone of the dentate gyrus of the hippocampus—and is supposedly involved in NSC niche regulation. In this review, we discuss the role of AKH as a niche molecule during mouse brain formation.

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Reciprocal Interaction between Vascular Filopodia and Neural Stem Cells Shapes Neurogenesis in the Ventral Telencephalon

Cited by 32 publications

References 57 publications

Apelin signaling dependent endocardial protrusions promote cardiac trabeculation in zebrafish

Apelin signaling dependent endocardial protrusions promote cardiac trabeculation in zebrafish

Transcriptome profiling of human pluripotent stem cell‐derived cerebellar organoids reveals faster commitment under dynamic conditions

Regulation of the Brain Neural Niche by Soluble Molecule Akhirin

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