Recessive and dominant genes confer resistance to<i>Colletotrichum truncatum</i>in cultivated lentil

Buchwaldt, L.; Shaikh, Rubeena; Adam, Jennifer; Tullu, A.; Slinkard, A. E.

doi:10.1080/07060661.2013.768296

Cited by 18 publications

(12 citation statements)

References 21 publications

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“…lentis could be a site of sRNA synthesis, that by one of the cited mechanisms could contribute to different pathogenicity of the two races, Ct0 and Ct1. Lentil accessions resistant to one or both races exist [ 8 , 21 ] and resistance genes against race Ct1 have been identified likely governed by the well known gene-for-gene interaction [ 36 ]. How fungal small RNA affects this interaction needs further investigation and is best carried out in a well characterized host-pathogen system amendable to gene recombination and genetic manipulation of one or both organisms.…”

Section: Discussionmentioning

confidence: 99%

IGS Minisatellites Useful for Race Differentiation in Colletotrichum lentis and a Likely Site of Small RNA Synthesis Affecting Pathogenicity

et al. 2015

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Colletotrichum lentis is a fungal pathogen of lentil in Canada but rarely reported elsewhere. Two races, Ct0 and Ct1, have been identified using differential lines. Our objective was to develop a PCR-probe differentiating these races. Sequences of the translation elongation factor 1α (tef1α), RNA polymerase II subunit B2 (rpb2), ATP citrate lyase subunit A (acla), and internal transcribed spacer (ITS) regions were monomorphic, while the intergenic spacer (IGS) region showed length polymorphisms at two minisatellites of 23 and 39 nucleotides (nt). A PCR-probe (39F/R) amplifying the 39 nt minisatellite was developed which subsequently revealed 1–5 minisatellites with 1–12 repeats in C. lentis. The probe differentiated race Ct1 isolates having 7, 9 or 7+9 repeats from race Ct0 having primarily 2 or 4 repeats, occasionally 5, 6, or 8, but never 7 or 9 repeats. These isolates were collected between 1991 and 1999. In a 2012 survey isolates with 2 and 4 repeats increased from 34% to 67%, while isolated with 7 or 9 repeats decreased from 40 to 4%, likely because Ct1 resistant lentil varieties had been grown. The 39 nt repeat was identified in C. gloeosporioides, C. trifolii, Ascochyta lentis, Sclerotinia sclerotiorum and Botrytis cinerea. Thus, the 39F/R PCR probe is not species specific, but can differentiate isolates based on repeat number. The 23 nt minisatellite in C. lentis exists as three length variants with ten sequence variations differentiating race Ct0 having 14 or 19 repeats from race Ct1 having 17 repeats, except for one isolate. RNA-translation of 23 nt repeats forms hairpins and has the appropriate length to suggest that IGS could be a site of small RNA synthesis, a hypothesis that warrants further investigation. Small RNA from fungal plant pathogens able to silence genes either in the host or pathogen thereby aiding infection have been reported.

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Section: Discussionmentioning

confidence: 99%

IGS Minisatellites Useful for Race Differentiation in Colletotrichum lentis and a Likely Site of Small RNA Synthesis Affecting Pathogenicity

et al. 2015

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“…These markers also were linked to a resistance locus in variety 'Indianhead'. The resistance loci are most likely CtR4 in PI320937 and CtR3 in 'Indianhead' described in the genetic study above (Buchwaldt et al, 2013). Simultaneously, Tar'an et al (2003) demonstrated the usefulness of markerassisted selection using a bi-parental F 7 population from a cross between varieties 'CDC Robin' (derived from 'Indianhead') with anthracnose resistance and breeding line 946a-46 (derived from accession ILL5588) with ascochyta resistance (Ford et al, 1999).…”

Section: Genetics Of Anthracnose Resistancementioning

confidence: 94%

“…A genetic study of inheritance showed that resistance to race Ct1 in L. culinaris 'Indianhead', PI320937 and PI345629 is conferred by a combination of recessive and dominant genes. So far, two recessive genes, ctr1 and ctr2, and three dominant genes, CtR3, CtR4 and CtR5, have been identified (Buchwaldt et al, 2013). Progenies in F 1 , F 2 , BC 1 and F 3 derived from crosses between each of the resistant lines and the susceptible cultivar, 'Eston', demonstrate that 'Indianhead' has one recessive and one dominant gene (ctr1, CtR3), PI345629 also has one recessive and one dominant gene (ctr2, CtR5) that are different from those in 'Indianhead', while PI320937 has a single dominant gene (CtR4) that is different from the others (Buchwaldt et al, 2013).…”

Section: Genetics Of Anthracnose Resistancementioning

confidence: 99%

“…So far, two recessive genes, ctr1 and ctr2, and three dominant genes, CtR3, CtR4 and CtR5, have been identified (Buchwaldt et al, 2013). Progenies in F 1 , F 2 , BC 1 and F 3 derived from crosses between each of the resistant lines and the susceptible cultivar, 'Eston', demonstrate that 'Indianhead' has one recessive and one dominant gene (ctr1, CtR3), PI345629 also has one recessive and one dominant gene (ctr2, CtR5) that are different from those in 'Indianhead', while PI320937 has a single dominant gene (CtR4) that is different from the others (Buchwaldt et al, 2013). Segregation ratios in F 2 from intercrossing the three resistant lines showed that the resistance genes are closely linked and likely different alleles at a single locus.…”

Section: Genetics Of Anthracnose Resistancementioning

confidence: 99%

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Lentil anthracnose: epidemiology, fungicide decision support system, resistance and pathogen races

Buchwaldt

Dzananovic

Durkin

2018

Canadian Journal of Plant Pathology

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Colletotrichum lentis causes anthracnose of lentil (Lens culinaris) in Canada that results in defoliation, stem girdling and severe yield losses, but the disease is rarely reported elsewhere. The pathogen survives as microsclerotia on lentil debris for up to 3 years when buried in the soil, but loses viability on the soil surface. Windborne debris spreads the pathogen to neighbouring fields, while seedborne infection is less important. Foliar fungicides were registered, and a fungicide decision support system was developed which assessed disease risk with 85% accuracy. Around 2300 L. culinaris accessions from 50 countries were screened for resistance. Congruently, two races-Ct1 and Ct0were identified on differential lentil lines. Resistant lines were generated by cycles of inoculation and selfing of single resistant plants which resulted in the following three accessions resistant to both races: VIR2633 (Georgia), VIR2058 and VIR2076 (Czech Republic), while six and 49 lines had resistance to Ct0 and Ct1, respectively. Ct1 resistance is controlled by recessive and dominant genes crt1 and CtR3 in variety 'Indianhead', ctr2 and CtR5 in accession PI345629 and CtR4 in PI320937. Molecular markers linked to Ct1 resistance were identified on linkage group six, close to Ascochyta lentis resistance, and were used to combine resistance to both pathogens in breeding lines. Two repeat rich regions in the intergenic spacer (IGS) of ribosomal DNA can be used to differentiate the two C. lentis races. Utilizing length polymorphisms in a 39 nucleotide repeat region showed the races were equally frequent among isolates collected between 1991 and 1999, while 95% belonged to race Ct0 in 2010, likely because lentil varieties are susceptible to race Ct0, but around one-third of the varieties had Ct1 resistance.

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“…The LCt-2 resistance locus was tagged with two fl anking RAPD markers at 6.4 and 10.5 cm ; in addition, three AFLP markers were also found to be linked to this locus (Tar'an et al 2003 ). However, most recently Buchwaldt et al ( 2013 ) have established that resistance to Ct1 race is controlled by two recessive genes ( lct-1 and lct-2 ) and three closely linked dominant genes .…”

Section: Biotic Stressesmentioning

confidence: 99%

Lentil

Fratini¹,

Vega²,

Sánchez³

2014

Broadening the Genetic Base of Grain Legumes

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Among the cool season legume crops, lentil production has displayed the greatest increase, while cropping of many other grain legumes is actually in decline. This increase is most likely due to a convenient fast cooking coupled to a saving of fuel and time -dehulled lentils cook even faster than milled rice. Worldwide lentil consumption has augmented more than twice the rate of human population growth, with lentil consumption over the past 40 years having increased more than any other food crop. Therefore, continued genetic improvement of lentil is essential in all production regions; nevertheless, without access to and use of diverse germplasm, prospects for yield genetic gain in lentil may be diffi cult to accomplish. Wild Lens accessions represent less than 1 % of the world germplasm collection of this genus, this small fraction likely representing the greatest untapped pool of genetic variation in lentil. Germplasm evaluation together with a successful and targeted hybridization allows for an effi cient breeding and selection of varieties adapted to specifi c environments. The aim of the present chapter is to describe the evolutionary aspects of lentil and assess the gene pool and fl ow for crop improvement, considering levels of diversity and agronomic traits of importance, with emphasis placed on wild species and interspecifi c Lens hybridization, to fi nally conclude with molecular aspects and future prospects of lentil breeding.

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Recessive and dominant genes confer resistance toColletotrichum truncatumin cultivated lentil

Cited by 18 publications

References 21 publications

IGS Minisatellites Useful for Race Differentiation in Colletotrichum lentis and a Likely Site of Small RNA Synthesis Affecting Pathogenicity

IGS Minisatellites Useful for Race Differentiation in Colletotrichum lentis and a Likely Site of Small RNA Synthesis Affecting Pathogenicity

Lentil anthracnose: epidemiology, fungicide decision support system, resistance and pathogen races

Lentil

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