Receptors for Histamine Can Be Detected on the Surface of Selected Leukocytes

Melmon, Kenneth L.; Bourne, Henry R.; Weinstein, Jacob J.; Sela, Michael

doi:10.1126/science.177.4050.707

Cited by 90 publications

(19 citation statements)

References 18 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…As one of the many important chemo-mediators involved in immune responses, histamine had inhibitory effects on immune response [35][36][37][38] via its H 2 receptors [39] . It was discovered that T suppressor cells, which are part of the regulatory arm of the immune system, could express histamine receptors on their surface [40][41][42] , and histamine was capable of suppressing the immune response by activating these T suppressor cells [43] . Many tumors, particularly colorectal cancer, secrete histamine resulting in elevated histamine levels within the tumor.…”

Section: Discussionmentioning

confidence: 99%

Perioperative cimetidine administration promotes peripheral blood lymphocytes and tumor infiltrating lymphocytes in patients with gastrointestinal cancer: Results of a randomized controlled clinical trial

Cai¹,

Bai²,

Yuan³

et al. 2004

WJG

View full text Add to dashboard Cite

AIM:To study the effects of perioperative administration of cimetidine (CIM) on peripheral blood lymphocytes, natural killer (NK) cells and tumor infiltrating lymphocytes (TIL) in patients with gastrointestinal (GI) cancer. METHODS:Forty-nine GI cancer patients were randomized into treatment group, who took CIM in perioperative period, and control group, who did not take the drug. The treatment was initiated 7 days before operation and continued for 10 days after surgery. At baseline examination before operation, on the 2nd and 10th postoperative days, total T lymphocytes, T helper cells, T suppressor cells, and NK cells in peripheral blood were measured respectively by immunocytochemical method using mouse-anti human CD 3 , CD 4 , CD 8 and CD 57 monoclonal antibodies. Blood samples from 20 healthy volunteers were treated in the same way as normal controls. Surgical specimens were examined during routine histopathological evaluation for the presence of TIL in tumor margin. Immunohistochemical study was performed to measure the proportion of T and B lymphocytes in TIL population. T and B lymphocytes were detected respectively using mouse-anti-human CD 3 and CD 20 monoclonal antibodies. RESULTS:In comparison with normal controls, both the treatment and control groups had decreased T cells, T helper cells and NK cells at baseline. In control group, total T cells, T helper cells and NK cells declined continuously with the disease progression and the decrease became more obvious after operation. From baseline to the 2nd postoperative day, the proportion of total T cells, T helper cells, and NK cells went down from 60.5±4.6% to 56.2±3.8%, 33.4±3.7% to 28.1±3.4%, and 15.0±2.8% to 14.2±2.2%, respectively. On the other hand, there were significant improvements in these parameters after CIM treatment. On the 10th postoperative day, the treatment group had significantly higher percentages of total T cells, T helper cells and NK cells than control group. Moreover, CIM treatment also boosted TIL response, as was reflected by findings that 68% (17/25) of the patients in treatment group had significant TIL responses and only 25% (6/24) of the cases had discernible TIL responses (P<0.01). CONCLUSION:Perioperative application of CIM to GI cancer patients could help restore the diminished cellular immunity induced by tumor burden and surgical maneuver. The drug could also boost TIL responses to tumor. These effects suggest that the drug be used as an immunomodulator for GI cancer patients.

show abstract

Section: Discussionmentioning

confidence: 99%

Perioperative cimetidine administration promotes peripheral blood lymphocytes and tumor infiltrating lymphocytes in patients with gastrointestinal cancer: Results of a randomized controlled clinical trial

Cai¹,

Bai²,

Yuan³

et al. 2004

WJG

View full text Add to dashboard Cite

show abstract

“…(c) Human leukocytes possess specific and separate receptors for endogenous hormones including B-adrenergic catecholamines, histamine, and prostaglandins (43). Each of these agents stimulates accumulation of cAMP in suspensions of mixed human leukocytes (15,44,46).…”

Section: Methodsmentioning

confidence: 99%

Mechanisms of Lysosomal Enzyme Release from Human Leukocytes II. EFFECTS OF cAMP AND cGMP, AUTONOMIC AGONISTS, AND AGENTS WHICH AFFECT MICROTUBULE FUNCTION

Zurier¹,

Weissmann²,

Hoffstein³

et al. 1974

J. Clin. Invest.

539

183

View full text Add to dashboard Cite

A B S T R A C T Selective release of inflammatory ma-terials from leukocyte lysosomes is reduced by compounds which increase cyclic 3',5'-adenosine monophosphate (cAMP) levels in suspensions of human leukocytes and is augmented by agents which increase cyclic 3',5'-guanosine monophosphate (cGMP) levels in these cell suspensions. Lysosomal enzymes are released in the absence of phagocytosis when cytochalasin B (5 ,ug/ml) converts polymorphonuclear leukocytes (PMN) to secretory cells: lysosomes merge directly with the plasma membrane upon encounter of PMN with zymosan, and cells selectively extrude substantial proportions of lysosomal, but not cytoplasmic enzymes. P-Adrenergic stimulation of human leukocytes produced a dose-related reduction in P-glucuronidase release (blocked by 10' M propranolol) whereas a-adrenergic stimulation (phenylephrine plus propranolol) was ineffective. In contrast, the cholinergic agonist carbamylcholine chloride enhanced enzyme secretion, an effect blocked by 1 0' M atropine. Incubation of cells with exogenous cAMP or with agents that increase endogenous cAMP levels (prostaglandin E1, histamine, isoproterenol, and cholera enterotoxin) reduced extrusion of lysosomal enzymes; in contrast, exogenous cGMP and carbamylcholine chloride (which increases endogenous cGMP levels), increased fi-glucuronidase release. Whereas colchicine (5 X 10-' M), a drug which impairs microtubule integrity, reduced selective enzyme release, deuterium oxide,

show abstract

“…Histamine receptors were demonstrated to be located on the plasma membrane of several mammalian cells (10,21,22,28,29,43). So far, their presence has been detected by the binding of various cells to histamine which was either insolubilized on THE JOURNAL OF CELL BIOLOGY " VOLUME 93 APRIL 1982 357-364 ©The Rockefeller University Press " 0021-9525/82/04/0357/08 $1 .00 albumin-treated agarose beads (28,46,47), or coupled to bovine serum albumin-fluorescein isothiocyanate (BSA-FITC) (12) or o-phtaldialdehyde (30).…”

mentioning

confidence: 99%

“…So far, their presence has been detected by the binding of various cells to histamine which was either insolubilized on THE JOURNAL OF CELL BIOLOGY " VOLUME 93 APRIL 1982 357-364 ©The Rockefeller University Press " 0021-9525/82/04/0357/08 $1 .00 albumin-treated agarose beads (28,46,47), or coupled to bovine serum albumin-fluorescein isothiocyanate (BSA-FITC) (12) or o-phtaldialdehyde (30). An indirect detection of Hr was acheived by determining the [3H]mepyramine binding (41).…”

mentioning

confidence: 99%

Histamine receptors of the microvascular endothelium revealed in situ with a histamine-ferritin conjugate: characteristic high-affinity binding sites in venules.

Heltianu¹,

Simionescu²,

Simiónescu³

1982

The Journal of Cell Biology

135

View full text Add to dashboard Cite

Histamine covalently bound to glutaraldehyde-activated ferritin was prepared as either monomers or as small aggregates of -0 .05 to 0.15 p,m Diam, suitable for electron microscopic detection of histamine cellular binding sites. The histamine-ferritin conjugates (MF) maintain the histamine capability to induce the opening of endothelial junctions in venules. To investigate the distribution of histamine receptors in the vascular endothelium, monomers or aggregates of MF were perfused in situ (mice), and various vascular beds, particularly that of the diaphragm, were fixed and processed for electron microscopy . The conjugate was preferentially bound on restricted areas of luminal endothelial cell plasmalemma especially in regions rich in filaments, and near the junctions between endothelial cells. The density of histamine binding sites was characteristically high in venules; it occurred to a much lesser extent in arterioles, veins, and muscular arteries whereas capillaries and aorta showed the lowest values . A similar distribution was obtained after perfusion of H, or H 2 receptor agonists coupled to ferritin (2-pyridylethylamine-ferritin [PF], or 4-methylhistamine-ferritin [MF], respectively) . The binding specificity was assessed through control experiments with either native or activated ferritin or by competition with histamine. The findings suggest that histamine receptors are largely represented in the cell membrane of the vascular endothelium, particularly in venules. Experiments using specific H, and H2 receptor agonists (PF and MF) and antagonists (mepyramine and cimetidine) indicate that the venular endothelium contains mainly H 2 receptors.

show abstract

Receptors for Histamine Can Be Detected on the Surface of Selected Leukocytes

Cited by 90 publications

References 18 publications

Perioperative cimetidine administration promotes peripheral blood lymphocytes and tumor infiltrating lymphocytes in patients with gastrointestinal cancer: Results of a randomized controlled clinical trial

Perioperative cimetidine administration promotes peripheral blood lymphocytes and tumor infiltrating lymphocytes in patients with gastrointestinal cancer: Results of a randomized controlled clinical trial

Mechanisms of Lysosomal Enzyme Release from Human Leukocytes II. EFFECTS OF cAMP AND cGMP, AUTONOMIC AGONISTS, AND AGENTS WHICH AFFECT MICROTUBULE FUNCTION

Histamine receptors of the microvascular endothelium revealed in situ with a histamine-ferritin conjugate: characteristic high-affinity binding sites in venules.

Contact Info

Product

Resources

About