Receptor Specificity Engineering of TNF Superfamily Ligands

Suo, Feng-Zhi; Zhou, Xinyu; Setroikromo, Rita; Quax, Wim J.

doi:10.3390/pharmaceutics14010181

Cited by 8 publications

(5 citation statements)

References 151 publications

(163 reference statements)

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The two primary mechanisms necessary for the induction of apoptosis are the intrinsic pathway and the extrinsic pathway mediated by the death receptor (regulated at the level of mitochondria). The Fas-associated death domain (FADD) is recruited through molecule signals (Fas / FasL) on the surface in the extrinsic pathway 48 . The procaspase-8 gene can be activated by the FADD-Fas complex.…”

Section: Resultsmentioning

confidence: 99%

Combined oncolytic virotherapy gold nanoparticles as synergistic immunotherapy agent in breast cancer control

Jabir,

Al-Shammari,

Ali

et al. 2023

Sci Rep

View full text Add to dashboard Cite

Combining viruses and nanoparticles may be a way to successfully treat cancer and minimize adverse effects. The current work aimed to evaluate the efficacy of a specific combination of gold nanoparticles (GNPs) and Newcastle disease virus (NDV) to enhance the antitumor effect of breast cancer in both in vitro and in vivo models. Two human breast cancer cell lines (MCF-7 and AMJ-13) and a normal epithelial cell line (HBL-100) were used and treated with NDV and/or GNPs. The MTT assay was used to study the anticancer potentials of NDV and GNP. The colony formation assay and apoptosis markers were used to confirm the killing mechanisms of NDV and GNP against breast cancer cell lines. p53 and caspase-9 expression tested by the qRT-PCR technique. Our results showed that combination therapy had a significant killing effect against breast cancer cells. The findings demonstrated that NDV and GNPs induced apoptosis in cancer cells by activating caspase-9, the p53 protein, and other proteins related to apoptosis, which holds promise as a combination therapy for breast cancer.

show abstract

Section: Resultsmentioning

confidence: 99%

Combined oncolytic virotherapy gold nanoparticles as synergistic immunotherapy agent in breast cancer control

Jabir,

Al-Shammari,

Ali

et al. 2023

Sci Rep

View full text Add to dashboard Cite

show abstract

“…The overall RSD value of 2.4% obtained for the current signals measured for the individual mixing of other cytokines with TNF-α and all together, with a maximum relative signal variation of 5%, confirmed that the selective detection of TNF-α cytokine could be achieved in the multicytokine sample matrix (Figure Biv). These findings were in agreement with early reports on cytokine–receptor interactions. , …”

Section: Resultsmentioning

confidence: 99%

“…These findings were in agreement with early reports on cytokine−receptor interactions. 33,34 The use of the specific detection antibody and standard HRP-TMB system enhanced the sensitivity of the biomembrane sensor. The analytical performance of the cell membrane-based bioelectronic chip for the sensitive detection of various TNF-α concentrations in the presence of IL-6 cytokine was also studied.…”

Section: Analytical Performance Of the Hybrid Cellmentioning

confidence: 99%

Using Cell Membranes as Recognition Layers to Construct Ultrasensitive and Selective Bioelectronic Affinity Sensors

et al. 2022

View full text Add to dashboard Cite

Conventional sandwich immunosensors rely on antibody recognition layers to selectively capture and detect target antigen analytes. However, the fabrication of these traditional affinity sensors is typically associated with lengthy and multistep surface modifications of electrodes and faces the challenge of nonspecific adsorption from complex sample matrices. Here, we report on a unique design of bioelectronic affinity sensors by using natural cell membranes as recognition layers for protein detection and prevention of biofouling. Specifically, we employ the human macrophage (MΦ) membrane together with the human red blood cell (RBC) membrane to coat electrochemical transducers through a one-step process. The natural protein receptors on the MΦ membrane are used to capture target antigens, while the RBC membrane effectively prevents nonspecific surface binding. In an attempt to detect tumor necrosis factor alpha (TNF-α) cytokine using the bioelectronic affinity sensor, it demonstrates a remarkable limit of detection of 150 pM. This new sensor design integrates natural cell membranes and electronic transduction, which offers synergistic functionalities toward a broad range of biosensing applications.

show abstract

“…As of today, the extrinsic and intrinsic pathways are the two main apoptosis pathways, and the perforin/granzyme pathway as a T-cell mediated signal route has also been defined. The extrinsic pathway is initiated through interactions of ligands with transmembrane receptors of the tumor necrosis factor (TNF) superfamily, such as TNF-α/TNFR1, FasL/Fas, TRAIL/DR4 or DR5, VEGI/DR3 and TWEAK/Fn14 [31][32][33][34][35][36][37]. The ligand binds to the receptor to recruit adaptor proteins (FADD, TRADD) to activate the caspase cascade and trigger apoptosis [38][39][40].…”

Section: Apoptosismentioning

confidence: 99%

Artemisinin-Type Drugs in Tumor Cell Death: Mechanisms, Combination Treatment with Biologics and Nanoparticle Delivery

et al. 2022

Self Cite

View full text Add to dashboard Cite

Artemisinin, the most famous anti-malaria drug initially extracted from Artemisia annua L., also exhibits anti-tumor properties in vivo and in vitro. To improve its solubility and bioavailability, multiple derivatives have been synthesized. However, to reveal the anti-tumor mechanism and improve the efficacy of these artemisinin-type drugs, studies have been conducted in recent years. In this review, we first provide an overview of the effect of artemisinin-type drugs on the regulated cell death pathways, which may uncover novel therapeutic approaches. Then, to overcome the shortcomings of artemisinin-type drugs, we summarize the recent advances in two different therapeutic approaches, namely the combination therapy with biologics influencing regulated cell death, and the use of nanocarriers as drug delivery systems. For the former approach, we discuss the superiority of combination treatments compared to monotherapy in tumor cells based on their effects on regulated cell death. For the latter approach, we give a systematic overview of nanocarrier design principles used to deliver artemisinin-type drugs, including inorganic-based nanoparticles, liposomes, micelles, polymer-based nanoparticles, carbon-based nanoparticles, nanostructured lipid carriers and niosomes. Both approaches have yielded promising findings in vitro and in vivo, providing a strong scientific basis for further study and upcoming clinical trials.

show abstract

Receptor Specificity Engineering of TNF Superfamily Ligands

Cited by 8 publications

References 151 publications

Combined oncolytic virotherapy gold nanoparticles as synergistic immunotherapy agent in breast cancer control

Combined oncolytic virotherapy gold nanoparticles as synergistic immunotherapy agent in breast cancer control

Using Cell Membranes as Recognition Layers to Construct Ultrasensitive and Selective Bioelectronic Affinity Sensors

Artemisinin-Type Drugs in Tumor Cell Death: Mechanisms, Combination Treatment with Biologics and Nanoparticle Delivery

Contact Info

Product

Resources

About