Receptor protein tyrosine kinase EphB4 is up-regulated in colon cancer

Stephenson, Sally‐Anne; Slomka, S.; Douglas, Evelyn; Hewett, Peter; Hardingham, Jennifer E.

doi:10.1186/1471-2199-2-15

Cited by 96 publications

(30 citation statements)

References 32 publications

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“…The Ephrin type B receptor 4 and its ligand Ephrin B2 appear to provide critical guidance cues at points of cell-cell contact in cardiovascular development. In adult settings of neo-angiogenesis, such as in tumors, it appears that the endothelium of a subset of new vessels strongly expresses the Ephrin B2 ligand (24), whereas the tumor cells express both the ligand and the receptor (25,26). Interestingly, it has been demonstrated that the Ephrin type B receptor 4 is up-regulated in colon tumors when compared with normal colon tissue from the same patient (26).…”

Section: Fig 4 Comparison Of the Ovarian Biotinylation Patterns To mentioning

confidence: 95%

“…In adult settings of neo-angiogenesis, such as in tumors, it appears that the endothelium of a subset of new vessels strongly expresses the Ephrin B2 ligand (24), whereas the tumor cells express both the ligand and the receptor (25,26). Interestingly, it has been demonstrated that the Ephrin type B receptor 4 is up-regulated in colon tumors when compared with normal colon tissue from the same patient (26). As such, the Ephrin type B receptor 4 may provide angiogenic signaling for the recruitment of new blood vessels to support tumor growth.…”

Section: Fig 4 Comparison Of the Ovarian Biotinylation Patterns To mentioning

confidence: 99%

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Global Profiling of the Cell Surface Proteome of Cancer Cells Uncovers an Abundance of Proteins with Chaperone Function

Shin¹,

Wang²,

Yim³

et al. 2003

Journal of Biological Chemistry

496

377

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There is currently limited data available pertaining to the global characterization of the cell surface proteome. We have implemented a strategy for the comprehensive profiling and identification of surface membrane proteins. This strategy has been applied to cancer cells, including the SH-SY5Y neuroblastoma, the A549 lung adenocarcinoma, the LoVo colon adenocarcinoma, and the Sup-B15 acute lymphoblastic leukemia (B cell) cell lines and ovarian tumor cells. Surface membrane proteins of viable, intact cells were subjected to biotinylation then affinity-captured and purified on monomeric avidin columns. The biotinylated proteins were eluted from the monomeric avidin columns as intact proteins and were subsequently separated by two-dimensional PAGE, transferred to polyvinylidene difluoride membranes, and visualized by hybridization with streptavidin-horseradish peroxidase. Highly reproducible, but distinct, two-dimensional patterns consisting of several hundred biotinylated proteins were obtained for the different cell populations analyzed. Identification of a subset of biotinylated proteins among the different cell populations analyzed using matrix-assisted laser desorption ionization and tandem mass spectrometry uncovered proteins with a restricted expression pattern in some cell line(s), such as CD87 and the activin receptor type IIB. We also identified more widely expressed proteins, such as CD98, and a sushi repeat-containing protein, a member of the selectin family. Remarkably, a set of proteins identified as chaperone proteins were found to be highly abundant on the cell surface, including GRP78, GRP75, HSP70, HSP60, HSP54, HSP27, and protein disulfide isomerase. Comprehensive profiling of the cell surface proteome provides an effective approach for the identification of commonly occurring proteins as well as proteins with restricted expression patterns in this compartment.

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Section: Fig 4 Comparison Of the Ovarian Biotinylation Patterns To mentioning

confidence: 95%

Section: Fig 4 Comparison Of the Ovarian Biotinylation Patterns To mentioning

confidence: 99%

Global Profiling of the Cell Surface Proteome of Cancer Cells Uncovers an Abundance of Proteins with Chaperone Function

Shin¹,

Wang²,

Yim³

et al. 2003

Journal of Biological Chemistry

496

377

View full text Add to dashboard Cite

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“…EphB4 is normally expressed on venous endothelial cells, while its exclusive ligand, EphrinB2, is expressed on arterial endothelial cells as a transmembrane protein as well. Aberrant expression of EphB4 has been observed in several tumor cell types, although its role in tumor cell biology remains to be fully elucidated (Andres et al, 1995;Berclaz et al, 1996Berclaz et al, , 2003Stephenson et al, 2001;Takai et al, 2001). EphB4 activation leads to a number of downstream effects, which influence cell attachment, migration and interaction with ligand-expressing cells (Adams et al, 1999;Oates et al, 1999;O'Leary and Wilkinson, 1999;Steinle et al, 2002).…”

Section: Introductionmentioning

confidence: 99%

EphB4 receptor tyrosine kinase is expressed in bladder cancer and provides signals for cell survival

et al. 2005

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“…They also play a role in angiogenesis (Favre et al, 2003;Sullivan and Bicknell, 2003). In recent years, some of the Eph genes have been found to be overexpressed in human tumors, including neuroblastoma (Tang et al, 1999), lung carcinoma (Tang et al, 1999), gastric cancer (Kiyokawa et al, 1994;Kataoka et al, 2002), esophageal cancer (Miyazaki et al, 2003), breast cancer (Wu et al, 2004), and colorectal cancer (Stephenson et al, 2001;Liu et al, 2002Liu et al, , 2004Kataoka et al, 2004). Among the Eph family genes, relatively less attention has been directed toward EphA7 in human tumors, and the potential role of EphA7 in human oncology has not been addressed.…”

Section: Introductionmentioning

confidence: 99%

Downregulation of EphA7 by hypermethylation in colorectal cancer

et al. 2005

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A significant reduction of EphA7 expression in human colorectal cancers was shown using semiquantitative reverse transcription-polymerase chain reaction analysis in 59 colorectal cancer tissues, compared to corresponding normal mucosas (P ¼ 0.008), and five colon cancer cell lines. To investigate the mechanism of EphA7 downregulation in colorectal cancer, we examined the methylation status of the 5 0 CpG island around the translation start site in five colon cancer cell lines using restriction enzymes, methylation-specific PCR, and bisulfite sequencing and found evidence of aberrant methylation. The expression of EphA7 in colon cancer cell lines was restored after treatment with 5-aza-2 0 -deoxycytidine. Analysis of methylation status in totally 75 tumors compared to clinicopathological parameters revealed that hypermethylation of colorectal cancers was more frequent in male than in female (P ¼ 0.0078), and in moderately differentiated than in well-differentiated adenocarcinomas (P ¼ 0.0361). There was a tendency that hypermethylation in rectal cancers was more frequent than in colon cancers (P ¼ 0.0816). Hypermethylation was also observed in colorectal adenomas. This is the first report describing the downregulation of an Eph family gene in a solid tumor via aberrant 5 0 CpG island methylation. It provides the evidence that EphA7 gene is involved in human colorectal carcinogenesis.

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Receptor protein tyrosine kinase EphB4 is up-regulated in colon cancer

Abstract: Background: We have used commercially available cDNA arrays to identify EphB4 as a gene that is up-regulated in colon cancer tissue when compared with matched normal tissue from the same patient.

Cited by 96 publications

References 32 publications

Global Profiling of the Cell Surface Proteome of Cancer Cells Uncovers an Abundance of Proteins with Chaperone Function

Global Profiling of the Cell Surface Proteome of Cancer Cells Uncovers an Abundance of Proteins with Chaperone Function

EphB4 receptor tyrosine kinase is expressed in bladder cancer and provides signals for cell survival

Downregulation of EphA7 by hypermethylation in colorectal cancer

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