Receptor-mediated cell mechanosensing

Chen, Yunfeng; Ju, Lining Arnold; Rushdi, Muaz Nik; Ge, Chenghao; Zhu, Cheng

doi:10.1091/mbc.e17-04-0228

Cited by 182 publications

(155 citation statements)

References 264 publications

Supporting

Mentioning

144

Contrasting

Order By: Relevance

“…However, it was obvious that surface of the films was not suitable for cells to attach and elongate, creating colonies. The latter can be achieved via adjacent cells through ligand engagement by receptor‐mediated cell mechanosensing (e.g., integrins and cadherins) . Judging from pictures a–d (Figure ), it was evident that there were no organized colonies, while pictures e–h (Figure ) clearly revealed round shaped cells.…”

Section: Resultsmentioning

confidence: 95%

“…The latter can be achieved via adjacent cells through ligand engagement by receptor-mediated cell mechanosensing (e.g., integrins and cadherins). [42] Judging from pictures a-d (Figure 9), it was evident that there were no organized colonies, while pictures e-h ( Figure 9) clearly revealed round shaped cells. The crosslinking method did not seem to make any difference in this case, and there was no obvious difference Macromol.…”

Section: Biocompatibility Studiesmentioning

confidence: 92%

See 1 more Smart Citation

Effect of Different Crosslinking Strategies on Physical Properties and Biocompatibility of Freestanding Multilayer Films Made of Alginate and Chitosan

Apte

Repanas

Willems

et al. 2019

Macromolecular Bioscience

View full text Add to dashboard Cite

Freestanding multilayer films prepared by layer‐by‐layer technique have attracted interest as promising materials for wound dressings. The goal is to fabricate freestanding films using chitosan (CHI) and alginate (ALG) including subsequent crosslinking to improve the mechanical properties of films while maintaining their biocompatibility. Three crosslinking strategies are investigated, namely use of calcium ions for crosslinking ALG, 1‐ethyl‐3‐(‐3‐dimethylaminopropyl) carbodiimide combined with N‐hydroxysuccinimide for crosslinking ALG with CHI, and Genipin for crosslinking chitosan inside the films. Different characteristics, such as surface morphology, wettability, swelling, roughness, and mechanical properties are investigated showing that films became thinner, exhibited rougher surfaces, had lower water uptake, and increased mechanical strength after crosslinking. Changes of wettability are moderate and dependent on the crosslinking method. In vitro cytotoxicity and cell attachment studies with human dermal fibroblasts show that freestanding CHI‐ALG films represent a poorly adhesive substratum for fibroblasts, while studies using incubation of plastic‐adherent fibroblast beneath floating films show no signs of cytotoxicity in a time frame of 7 days. Results from cell experiments combined with film characteristics after crosslinking, indicate that crosslinked freestanding films made of ALG and CHI may be interesting candidates for wound dressings.

show abstract

Section: Resultsmentioning

confidence: 95%

Section: Biocompatibility Studiesmentioning

confidence: 92%

Effect of Different Crosslinking Strategies on Physical Properties and Biocompatibility of Freestanding Multilayer Films Made of Alginate and Chitosan

Apte

Repanas

Willems

et al. 2019

Macromolecular Bioscience

View full text Add to dashboard Cite

show abstract

“…For example, branch extension of microglia is directly correlated with the stiffness of the surrounding microenvironment (28). Mechanical stimuli up-regulate the expression of microglial integrin-b1 (29), a membrane receptor involved in cell mechanosensing (30), thus promoting microglia branch extension (31). Integrin-b1 is commonly associated with an Rac GTPasedependent cytoskeletal rearrangement (32) and may be activated by cell-extracellular matrix (33)(34)(35) and cell-cell interactions (36).…”

mentioning

confidence: 99%

Microglial distribution, branching, and clearance activity in aged rat hippocampus are affected by astrocyte meshwork integrity: evidence of a novel cell‐cell interglial interaction

et al. 2018

View full text Add to dashboard Cite

Aging and neurodegenerative diseases share a condition of neuroinflammation entailing the production of endogenous cell debris in the CNS that must be removed by microglia (i.e., resident macrophages), to restore tissue homeostasis. In this context, extension of microglial cell branches toward cell debris underlies the mechanisms of microglial migration and phagocytosis. Amoeboid morphology and the consequent loss of microglial branch functionality characterizes dysregulated microglia. Microglial migration is assisted by another glial population, the astroglia, which forms a dense meshwork of cytoplasmic projections. Amoeboid microglia and disrupted astrocyte meshwork are consistent traits in aged CNS. In this study, we assessed a possible correlation between microglia and astroglia morphology in rat models of chronic neuroinflammation and aging, by 3‐dimensional confocal analysis implemented with particle analysis. Our findings suggest that a microglia‐astroglia interaction occurs in rat hippocampus via cell‐cell contacts, mediating microglial cell branching in the presence of inflammation. In aged rats, the impairment of such an interaction correlates with altered distribution, morphology, and inefficient clearance by microglia. These data support the idea that generally accepted functional boundaries between microglia and astrocytes should be re‐evaluated to better understand how their functions overlap and interact.—Lana, D., Ugolini, F., Wenk, G. L., Giovannini, M. G., Zecchi‐Orlandini, S., Nosi, D. Microglial distribution, branching, and clearance activity in aged rat hippocampus are affected by astrocyte meshwork integrity: evidence of a novel cell‐cell interglial interaction. FASEB J. 33, 4007–4020 (2019). http://www.fasebj.org

show abstract

“…These assays have been previously described 1,13,30 . In these assays, the RP1 aspirated cell is driven to repeatedly contact the probe bead for 2 s and retract at a constant speed (3.33 μm/s).…”

Section: Red Blood Cell and Platelet Isolation And Preparation Humanmentioning

confidence: 99%

“…Two types of methods have been developed for manipulating, visualizing, and quantitatively analyzing receptor-ligand interactions on single cell in real-time 9,10 . One type is mechanically based, using pico-force techniques, such as the commercially available atomic force microscope and optical traps 11 as well as the user-made micropipette-based biomembrane force probe (BFP) 1,12,13 . The other type is fluorescently based, using sensitive imaging systems, such as that used on hybrid junctions between live cells and supported lipid bilayer 14,15 .…”

mentioning

confidence: 99%

Dual Biomembrane Force Probe Enables Single-Cell Mechanical Analysis of Signal Crosstalk between Multiple Molecular Species

Chen

et al. 2018

Biophysical Journal

Self Cite

View full text Add to dashboard Cite

Conventional approaches for studying receptor-mediated cell signaling, such as the western blot and flow cytometry, are limited in three aspects: 1) The perturbing preparation procedures often alter the molecules from their native state on the cell; 2) Long processing time before the final readout makes it difficult to capture transient signaling events (<1 min); 3) The experimental environments are force-free, therefore unable to visualize mechanical signals in real time. In contrast to these methods in biochemistry and cell biology that are usually population-averaged and non-real-time, here we introduce a novel single-cell based nanotool termed dual biomembrane force probe (dBFP). The dBFP provides precise controls and quantitative readouts in both mechanical and chemical terms, which is particularly suited for juxtacrine signaling and mechanosensing studies. Specifically, the dBFP allows us to analyze dual receptor crosstalk by quantifying the spatiotemporal requirements and functional consequences of the up-and down-stream signaling events. In this work, the utility and power of the dBFP has been demonstrated in four important dual receptor systems that play key roles in immunological synapse formation, shear-dependent thrombus formation, and agonist-driven blood clotting.Over the past decade, single-molecule biomechanical analyses on single cells have enabled studies of the inner workings of adhesion and signaling receptors one at a time 1 . In physiological conditions, however, multiple receptor species are present and often work cooperatively rather than independently. Understanding how multiple receptor species crosstalk to each other is essential for determining the mechanisms underlying a wide range of biological processes related to human health and diseases. One of the model receptor systems that exhibit such signaling crosstalk with other receptors is the integrin family. For example, integrin α L β 2 , or lymphocyte function-associated antigen-1 (LFA-1), crosstalks with chemokine receptors and antigen receptors via an "inside-out" signaling process 2 , which is essential for lymphocyte trafficking and immunological synapse formation 3 . The interaction between LFA-1 on a lymphocyte and its ligand, intercellular adhesion molecule-1 (ICAM-1) on an adjacent cell, can be upregulated by signals induced by binding of chemokines and antigens to their respective receptors on the same lymphocyte, manifesting enhanced cell adhesion 4,5 . As another example, upon a rapid shear force increase caused by blood flow perturbations, the function of integrin α IIb β 3 , or glycoprotein (GP) IIb-IIIa, on a platelet surface is rapidly upregulated by signals induced by ligand engagement with the mechanoreceptor GPIb 6,7 , which promotes platelet adhesion at the site of vascular injury as part of the hemostatic and thrombotic processes 8 . The synergistic binding of GPIb and GPIIb-IIIa enables efficient platelet recruitment

show abstract

Receptor-mediated cell mechanosensing

Cited by 182 publications

References 264 publications

Effect of Different Crosslinking Strategies on Physical Properties and Biocompatibility of Freestanding Multilayer Films Made of Alginate and Chitosan

Effect of Different Crosslinking Strategies on Physical Properties and Biocompatibility of Freestanding Multilayer Films Made of Alginate and Chitosan

Microglial distribution, branching, and clearance activity in aged rat hippocampus are affected by astrocyte meshwork integrity: evidence of a novel cell‐cell interglial interaction

Dual Biomembrane Force Probe Enables Single-Cell Mechanical Analysis of Signal Crosstalk between Multiple Molecular Species

Contact Info

Product

Resources

About