Receptor-like protein kinases in plant reproduction: Current understanding and future perspectives

Cui, Yanwei; Lu, Xiaoting; Gou, Xiaoping

doi:10.1016/j.xplc.2021.100273

Cited by 32 publications

(19 citation statements)

References 203 publications

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“…How the EPFL signals are transduced to control integument morphogenesis after they are perceived by ERf receptors is another important question. A SERK or CIK coreceptor is often recruited by a ligand‐binding RLK with long extracellular domain to fully activate the signaling pathways (Gou & Li, 2020; Cui et al ., 2022). It was noticed that serk1/2/3 mutants were female‐sterile (Fig.…”

Section: Discussionmentioning

confidence: 99%

The EPFL–ERf–SERK signaling controls integument development in Arabidopsis

Wang

et al. 2023

New Phytologist

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Summary As the seed precursor, the ovule produces the female gametophyte (or embryo sac), and the subsequent double fertilization occurs in it. The integuments emerge sequentially from the integument primordia at the early stages of ovule development and finally enwrap the embryo sac gradually during gametogenesis, protecting and nursing the embryo sac. However, the mechanisms regulating integument development are still obscure. In this study, we show that SOMATIC EMBRYOGENESIS RECEPTOR‐LIKE KINASES (SERKs) play essential roles during integument development in Arabidopsis thaliana. The serk1/2/3 triple mutant shows arrested integuments and abnormal embryo sacs, similar defects also found in the triple loss‐of‐function mutants of ERECTA family (ERf) genes. Ovules of serk1/2/3 er erl1/2 show defects similar to er erl1/2 and serk1/2/3. Results of yeast two‐hybrid analyses, bimolecular fluorescence complementation (BiFC) analyses, and co‐immunoprecipitation assays demonstrated that SERKs interact with ERf, which depends on EPIDERMAL PATTERNING FACTOR‐LIKE (EPFL) family small peptides. The sextuple mutant epfl1/2/3/4/5/6 shows integument defects similar to both of er erl1/2 and serk1/2/3. Our results demonstrate that ERf–SERK‐mediated EPFL signaling orchestrates the development of the female gametophyte and the surrounding sporophytic integuments.

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Section: Discussionmentioning

confidence: 99%

The EPFL–ERf–SERK signaling controls integument development in Arabidopsis

Wang

et al. 2023

New Phytologist

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“…AGP24 co-expression data showed some interesting genes from other gene families that are important for plant reproduction such as RECEPTOR-LIKE SERINE/THREONINE KINASE 2 (RKF2; AT1G19090) or CRK1 (CYSTEINE-RICH RLK) and CALCIUM-DEPENDENT PROTEIN KINASE 26 (ATCPK26; AT4G38230), belonging to the calcium-dependent protein kinases family (Ca 2+ /CPKs), vital components in the Ca 2+ signaling pathways. Receptor-like protein kinases (RLKs) are a large family of membrane proteins essential for reproductive development (Cui et al, 2022), and Ca 2+ is described as an important ion in the entire reproductive process and has also been related to the putative functions and mode of action of AGPs (Cheng et al, 2002;Lamport and Vaŕnai, 2013). A recent study clearly highlighted AGPs as essential players involved in novel molecular pinball machines of the plasma membrane controlling cytosolic Ca 2+ levels that regulate plant metabolism (Lamport et al, 2021).…”

Section: Discussionmentioning

confidence: 99%

New insights on the expression patterns of specific Arabinogalactan proteins in reproductive tissues of Arabidopsis thaliana

et al. 2022

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Arabinogalactan proteins (AGPs) are hydroxyproline-rich glycoproteins containing a high proportion of carbohydrates, widely distributed in the plant kingdom and ubiquitously present in land plants. AGPs have long been suggested to play important roles in plant reproduction and there is already evidence that specific glycoproteins are essential for male and female gametophyte development, pollen tube growth and guidance, and successful fertilization. However, the functions of many of these proteins have yet to be uncovered, mainly due to the difficulty to study individual AGPs. In this work, we generated molecular tools to analyze the expression patterns of a subgroup of individual AGPs in different Arabidopsis tissues, focusing on reproductive processes. This study focused on six AGPs: four classical AGPs (AGP7, AGP25, AGP26, AGP27), one AG peptide (AGP24) and one chimeric AGP (AGP31). These AGPs were first selected based on their predicted expression patterns along the reproductive tissues from available RNA-seq data. Promoter analysis using β-glucuronidase fusions and qPCR in different Arabidopsis tissues allowed to confirm these predictions. AGP7 was mainly expressed in female reproductive tissues, more precisely in the style, funiculus, and integuments near the micropyle region. AGP25 was found to be expressed in the style, septum and ovules with higher expression in the chalaza and funiculus tissues. AGP26 was present in the ovules and pistil valves. AGP27 was expressed in the transmitting tissue, septum and funiculus during seed development. AGP24 was expressed in pollen grains, in mature embryo sacs, with highest expression at the chalazal pole and in the micropyle. AGP31 was expressed in the mature embryo sac with highest expression at the chalaza and, occasionally, in the micropyle. For all these AGPs a co-expression analysis was performed providing new hints on its possible functions. This work confirmed the detection in Arabidopsis male and female tissues of six AGPs never studied before regarding the reproductive process. These results provide novel evidence on the possible involvement of specific AGPs in plant reproduction, as strong candidates to participate in pollen-pistil interactions in an active way, which is significant for this field of study.

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“…Glysoja.15G042020 and Glysoja.15G042019, which encode Glutathione Stransferase, were detected in the 48099201-48129356 region on chromosomes 15. A number of studies have reported that Glutathione S-transferase, caffeoyl-CoA O-methyltransferase, LRR receptor-like serine/threonine protein kinase, and receptor-like protein kinases play important roles in plant defense-related reactions against fungal attacks [43][44][45][46][47][48]. Jing et al (2015) found that the expression of the GST family in soybeans was down-regulated following P. sojae infections [49].…”

Section: Discussionmentioning

confidence: 99%

Genome-Wide Association Study of Partial Resistance to P. sojae in Wild Soybeans from Heilongjiang Province, China

Liu

Lai

et al. 2022

CIMB

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Phytophthora root rot (PRR) is a destructive disease of soybeans (Glycine max (L.) Merr) caused by Phytophthora sojae (P. sojae). The most effective way to prevent the disease is growing resistant or tolerant varieties. Partial resistance provides a more durable resistance against the pathogen compared to complete resistance. Wild soybean (Glycine soja Sieb. & Zucc.) seems to be an extraordinarily important gene pool for soybean improvement due to its high level of genetic variation. In this study, 242 wild soybean germplasms originating from different regions of Heilongjiang province were used to identify resistance genes to P. sojae race 1 using a genome-wide association study (GWAS). A total of nine significant SNPs were detected, repeatedly associated with P. sojae resistance and located on chromosomes 1, 10, 12, 15, 17, 19 and 20. Among them, seven favorable allelic variations associated with P. sojae resistance were evaluated by a t-test. Eight candidate genes were predicted to explore the mechanistic hypotheses of partial resistance, including Glysoja.19G051583, which encodes an LRR receptor-like serine/threonine protein kinase protein, Glysoja.19G051581, which encodes a receptor-like cytosolic serine/threonine protein kinase protein. These findings will provide additional insights into the genetic architecture of P. sojae resistance in a large sample of wild soybeans and P. sojae-resistant breeding through marker-assisted selection.

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Receptor-like protein kinases in plant reproduction: Current understanding and future perspectives

Cited by 32 publications

References 203 publications

The EPFL–ERf–SERK signaling controls integument development in Arabidopsis

The EPFL–ERf–SERK signaling controls integument development in Arabidopsis

New insights on the expression patterns of specific Arabinogalactan proteins in reproductive tissues of Arabidopsis thaliana

Genome-Wide Association Study of Partial Resistance to P. sojae in Wild Soybeans from Heilongjiang Province, China

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