Receptor-like kinases and receptor-like proteins: keys to pathogen recognition and defense signaling in plant innate immunity

Yang, Xin; Deng, Fengyang; Ramonell, Katrina M.

doi:10.1007/s11515-011-1185-8

Cited by 37 publications

(37 citation statements)

References 88 publications

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“…Several WAKs bear a calcium-binding domain and their up-regulation in GV contributed to the enrichment of the GO term “calcium ion binding” (GO:0005509). In Arabidopsis, WAKs are involved in cell expansion, pathogen resistance and heavy metal stress tolerance [55], while AtWAK1 potentially acts as a receptor of oligogalacturonides elicitors [56]. WAKs link the plasma membrane to the cell wall matrix through their extracellular receptor domain, and can link perception of external signals (including pathogen attacks) to the triggering of defence mechanisms via their cytoplasmic kinase domains [57].…”

Section: Resultsmentioning

confidence: 99%

Comparative Transcriptome Profiling of the Early Response to Magnaporthe oryzae in Durable Resistant vs Susceptible Rice (Oryza sativa L.) Genotypes

Bagnaresi¹,

Biselli²,

Orrù³

et al. 2012

PLoS ONE

139

136

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Durable resistance to blast, the most significant fungal disease of rice, represents an agronomically relevant character. Gigante Vercelli (GV) and Vialone Nano (VN) are two old temperate japonica Italian rice cultivars with contrasting response to blast infection: GV displays durable and broad resistance while VN is highly susceptible. RNA-seq was used to dissect the early molecular processes deployed during the resistance response of GV at 24 h after blast inoculation. Differential gene expression analysis identified 1,070 and 1,484 modulated genes, of which 726 and 699 were up regulated in response to infection in GV and VN, respectively. Gene ontology (GO) enrichment analyses revealed a set of GO terms enriched in both varieties but, despite this commonality, the gene sets contributing to common GO enriched terms were dissimilar. The expression patterns of genes grouped in GV-specific enriched GO terms were examined in detail including at the transcript isoform level. GV exhibited a dramatic up-regulation of genes encoding diterpene phytoalexin biosynthetic enzymes, flavin-containing monooxygenase, class I chitinase and glycosyl hydrolase 17. The sensitivity and high dynamic range of RNA-seq allowed the identification of genes critically involved in conferring GV resistance during the early steps of defence perception-signalling. These included chitin oligosaccharides sensing factors, wall associated kinases, MAPK cascades and WRKY transcription factors. Candidate genes with expression patterns consistent with a potential role as GV-specific functional resistance (R) gene(s) were also identified. This first application of RNA-seq to dissect durable blast resistance supports a crucial role of the prompt induction of a battery of responses including defence-related genes as well as members of gene families involved in signalling and pathogen-related gene expression regulation.

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Section: Resultsmentioning

confidence: 99%

Comparative Transcriptome Profiling of the Early Response to Magnaporthe oryzae in Durable Resistant vs Susceptible Rice (Oryza sativa L.) Genotypes

Bagnaresi¹,

Biselli²,

Orrù³

et al. 2012

PLoS ONE

139

136

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“…The best studied examples are FLAGELLIN SENSING2 (FLS2) and EF-TU RECEPTOR (EFR), two receptors with an extracellular domain harboring tandem leucine-rich repeats (eLRRs) that bind an ;22-amino acid fragment of bacterial flagellin, flg22, and an ;18-amino acid fragment of bacterial elongation factor thermo-unstable, elf18, respectively (GomezGomez and Boller, 2000;Zipfel et al, 2006). In addition to RLKs, receptor-like proteins (RLPs) function as crucial players in plant immunity (Yang et al, 2012). RLPs and RLKs have similar structures, except that the RLPs lack the cytoplasmic kinase domain (Wang et al, 2008).…”

Section: Introductionmentioning

confidence: 99%

ArabidopsisRECEPTOR-LIKE PROTEIN30 and Receptor-Like Kinase SUPPRESSOR OF BIR1-1/EVERSHED Mediate Innate Immunity to Necrotrophic Fungi

et al. 2013

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Effective plant defense strategies rely in part on the perception of non-self determinants, so-called microbe-associated molecular patterns (MAMPs), by transmembrane pattern recognition receptors leading to MAMP-triggered immunity. Plant resistance against necrotrophic pathogens with a broad host range is complex and yet not well understood. Particularly, it is unclear if resistance to necrotrophs involves pattern recognition receptors. Here, we partially purified a novel proteinaceous elicitor called SCLEROTINIA CULTURE FILTRATE ELICITOR1 (SCFE1) from the necrotrophic fungal pathogen Sclerotinia sclerotiorum that induces typical MAMP-triggered immune responses in Arabidopsis thaliana. Analysis of natural genetic variation revealed five Arabidopsis accessions (Mt-0, Lov-1, Lov-5, Br-0, and Sq-1) that are fully insensitive to the SCFE1-containing fraction. We used a forward genetics approach and mapped the locus determining SCFE1 sensitivity to RECEPTOR-LIKE PROTEIN30 (RLP30). We also show that SCFE1-triggered immune responses engage a signaling pathway dependent on the regulatory receptor-like kinases BRASSINOSTEROID INSENSITIVE1-ASSOCIATED RECEPTOR KINASE1 (BAK1) and SUPPRESSOR OF BIR1-1/EVERSHED (SOBIR1/EVR). Mutants of RLP30, BAK1, and SOBIR1 are more susceptible to S. sclerotiorum and the related fungus Botrytis cinerea. The presence of an elicitor in S. sclerotiorum evoking MAMPtriggered immune responses and sensed by RLP30/SOBIR1/BAK1 demonstrates the relevance of MAMP-triggered immunity in resistance to necrotrophic fungi.

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“…As the Figure 6A showed, among the top 50 hub genes, the serine/threonine protein kinase D6PK and the LRR-RLK CLAVATA2 were strongest, with 2611 and 1752 connections, respectively (Figure 6A). LRR-RLKs are reportedly involved in plant defense responses against various classes of pathogens and may initiate the activation of the MAPK pathway, WRKY transcription factors, ion channels and the NADPH oxidase complex (Yang et al, 2012). The LRR-RLK2 CLAVATA has been reported to confer enhanced disease resistance to bacterial wilt (Hanemian et al, 2016).…”

Section: Discussionmentioning

confidence: 99%

“…At the bottom of the hierarchical clustering, the expression of a small set of LRR-RLKs was induced in V. montana but repressed in V. fordii . LRR-RLKs have been reported to have key roles in pathogen recognition, and they may lead to the activation of ion channels, the NADPH oxidase complex and MAPK cascades after trans-phosphorylation and phosphorylation events (Yang et al, 2012). Similarly, we conducted gene enrichment analysis on 450 unigenes and also clearly identified the above pathways (Figure 5).…”

Section: Discussionmentioning

confidence: 99%

Comparative Transcriptomics Atlases Reveals Different Gene Expression Pattern Related to Fusarium Wilt Disease Resistance and Susceptibility in Two Vernicia Species

et al. 2016

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Vernicia fordii (tung oil tree) is a promising industrial crop. Unfortunately, the devastating Fusarium wilt disease has caused its great losses, while its sister species (Vernicia montana) is remarkably resistant to this pathogen. However, the genetic mechanisms underlying this difference remain largely unknown. We here generated comparative transcriptomic atlases for different stages of Fusarium oxysporum infected Vernicia root. The transcriptomes of V. fordii and V. montana were assembled de novo and contained 258,430 and 245,240 non-redundant transcripts with N50 values of 1776 and 2452, respectively. A total of 44,310 pairs of putative one-to-one orthologous genes were identified in Vernicia species. Overall, the vast majority of orthologous genes shared a remarkably similar expression mode. The expression patterns of a small set of genes were further validated by quantitative real-time PCR. Moreover, 157 unigenes whose expression significantly correlated between the two species were defined, and gene set enrichment analysis indicated roles in increased defense response and in jasmonic and salicylic acid signaling responses during pathogen attack. Co-expression network analysis further identified the 17 hub unigenes, such as the serine/threonine protein kinase D6PK, leucine-rich repeat receptor-like kinase (LRR-RLK), and EREBP transcription factor, which play essential roles in plant pathogen resistance. Intriguingly, the expression of most hub genes differed significantly between V. montana and V. fordii. Based on our results, we propose a model to describe the major molecular reactions that underlie the defense responses of resistant V. montana to F. oxysporum. These data represent a crucial step toward breeding more pathogen-resistant V. fordii.

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Receptor-like kinases and receptor-like proteins: keys to pathogen recognition and defense signaling in plant innate immunity

Cited by 37 publications

References 88 publications

Comparative Transcriptome Profiling of the Early Response to Magnaporthe oryzae in Durable Resistant vs Susceptible Rice (Oryza sativa L.) Genotypes

Comparative Transcriptome Profiling of the Early Response to Magnaporthe oryzae in Durable Resistant vs Susceptible Rice (Oryza sativa L.) Genotypes

ArabidopsisRECEPTOR-LIKE PROTEIN30 and Receptor-Like Kinase SUPPRESSOR OF BIR1-1/EVERSHED Mediate Innate Immunity to Necrotrophic Fungi

Comparative Transcriptomics Atlases Reveals Different Gene Expression Pattern Related to Fusarium Wilt Disease Resistance and Susceptibility in Two Vernicia Species

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