Receptor-like cytoplasmic kinase MAZZA mediates developmental processes with CLAVATA1 family receptors in Arabidopsis

Abstract

The receptor-like kinases (RLKs) CLAVATA1 (CLV1) and BARELY ANY MERISTEMs (BAM1–BAM3) form the CLV1 family (CLV1f), which perceives peptides of the CLV3/EMBRYO SURROUNDING REGION (ESR)-related (CLE) family within various signaling pathways of Arabidopsis thaliana. CLE peptide signaling, which is required for meristem size control, vascular development, and pathogen responses, involves the formation of receptor complexes at the plasma membrane. These complexes comprise RLKs and co-receptors in varying compositions depending on the signaling context, and regulate expression of target genes, such as WUSCHEL (WUS). How the CLE signal is transmitted intracellularly after perception at the plasma membrane is not known in detail. Here, we found that the membrane-associated receptor-like cytoplasmic kinase (RLCK) MAZZA (MAZ) and additional members of the Pti1-like protein family interact in vivo with CLV1f receptors. MAZ, which is widely expressed throughout the plant, localizes to the plasma membrane via post-translational palmitoylation, potentially enabling stimulus-triggered protein re-localization. We identified a role for a CLV1–MAZ signaling module during stomatal and root development, and redundancy could potentially mask other phenotypes of maz mutants. We propose that MAZ, and related RLCKs, mediate CLV1f signaling in a variety of developmental contexts, paving the way towards understanding the intracellular processes after CLE peptide perception.

CLE3 and its homologs share overlapping functions in the modulation of lateral root formation through CLV1 and BAM1 in Arabidopsis thaliana

“…To gain insights into how CLV1 and BAM1 may impact the lateral root formation, we investigated the cell‐type expression pattern of CLV1‐GFP and BAM1‐GFP proteins during LRP development. Confocal microscopy analysis showed that CLV1‐GFP fusion protein was indeed localized to the phloem companion cells in the basal regions of LRP and emerged lateral root (Figure 5e), consistent with the literature (Araya et al., 2014; Blümke et al., 2021). In addition, CLV1‐GFP signal was also observed in the companion cells adjacent to LRP and emerged lateral root (Figure S5d).…”

TPLATE complex dependent endocytosis is required for shoot apical meristem maintenance by attenuating CLAVATA1 signaling

“…In vitro bioassays comparing root growth in the presence or absence of exogenous peptide ligands provide an easy readout and are widely employed to evaluate how plants respond to peptide-dependent signaling (Anne et al , 2018; Blumke et al , 2021; Breda et al , 2019; Graeff et al , 2020; Hazak et al , 2017; Hu et al , 2018; Poncini et al , 2017). To correlate peptide-dependent receptor signalling with CME capacity, we selected several classes of peptide ligands.…”

OsSWEET11b, a potential sixth leaf blight susceptibility gene involved in sugar transport‐dependent male fertility

“…Agrobacterium culture preparation and tobacco leaf infiltration were performed as described (Sosso et al ., 2015). As a control, pAB118:AtMAZZA:mCherry carrying the coding sequence of Arabidopsis MAZZA gene fused at the C‐terminus with mCherry driven by the β‐estradiol inducible CaMV 35S promoter was used (Blümke et al ., 2021). Fluorescence was detected on an Olympus SpinSR (Olympus Corp., Hamburg, Germany) with excitation/emission 488/522–572 nm (eGFP) and 561/667–773 nm (chlorophyll).…”