Receptor interference groups of 20 retroviruses plating on human cells

Sommerfelt, Maja A.; Weiss, Robin A.

doi:10.1016/0042-6822(90)90230-o

Cited by 229 publications

(197 citation statements)

References 68 publications

Supporting

Mentioning

179

Contrasting

Order By: Relevance

“…This process, termed interference, apparently involves sequestration, surface down-regulation, or masking of gD receptors that are essential for HSV entry (30). These results were similar to earlier observations of receptor interference where retrovirus-infected cells were resistant to infection by related retroviruses that used the same entry receptors (31). Therefore, interference provides strong evidence for cellular receptors or entry mediators that are bound by specific viral proteins.…”

Section: Hcmv Glycoproteins ͉ Receptor Interferencesupporting

confidence: 83%

“…Experiments involving radiolabeled virus particles and PEG treatment demonstrated that interference did not block virus adsorption onto cells but instead inhibited subsequent stages of HCMV entry into cells. Thus, gH/gL/UL128-131-mediated interference appears to be similar to the interference mediated by the HSV receptor-binding protein gD (28)(29)(30) and retrovirus interference involving common receptors (31). Our observations support the hypothesis that gH/gL/UL128-131 promotes entry into epithelial cells by binding receptors or entry mediators, molecules that can be masked, sequestered, or down-regulated by gH/gL/UL128-131 expression.…”

Section: Discussionmentioning

confidence: 88%

See 1 more Smart Citation

HCMV gH/gL/UL128–131 interferes with virus entry into epithelial cells: Evidence for cell type-specific receptors

Ryckman

Chase

Johnson

2008

Proc. Natl. Acad. Sci. U.S.A.

147

159

View full text Add to dashboard Cite

Human cytomegalovirus (HCMV) forms two different membrane protein complexes, gH/gL/gO and gH/gL/UL128/UL130/UL131, that function in different cell types. gH/gL/gO appears to be important for HCMV entry into or spread between fibroblasts, processes that occur at neutral pH. We demonstrated that HCMV entry into epithelial and endothelial cells requires gH/gL/UL128 -131 and involves endocytosis and low pH. A complex of all five HCMV proteins, gH, gL, UL128, UL130, and UL131, is the functionally important mediator of this entry pathway into epithelial/endothelial cells. Here, we report that expression of gH/gL/UL128 -131 in ARPE-19 epithelial cells causes the cells to be resistant to HCMV infection. Another HCMV glycoprotein, gB, did not interfere, and expression of all five gH/gL/UL128 -131 proteins was required for this interference. gH/gL/UL128 -131 interference was at the stage of virus entry into cells rather than the initial adsorption onto cell surfaces or after-entry defects. By contrast, expression of gH/gL/ UL128 -131 in primary human fibroblasts did not block HCMV infection. Previously, interference by retrovirus and herpes-simplex-virus entry mediators resulted from sequestration or obstruction of receptors. We concluded that epithelial cells express gH/ gL/UL128 -131 receptors that mediate HCMV entry. Fibroblasts either lack the gH/gL/UL128 -131 receptors, the receptors are more numerous, or fibroblasts express other functional receptors.HCMV glycoproteins ͉ receptor interference

show abstract

Section: Hcmv Glycoproteins ͉ Receptor Interferencesupporting

confidence: 83%

Section: Discussionmentioning

confidence: 88%

HCMV gH/gL/UL128–131 interferes with virus entry into epithelial cells: Evidence for cell type-specific receptors

Ryckman

Chase

Johnson

2008

Proc. Natl. Acad. Sci. U.S.A.

147

159

View full text Add to dashboard Cite

show abstract

“…Interference assays demonstrated that an immunoadhesin, wherein this SU domain was fused to a rabbit Ig Fc (rFc) tag, was able to bind the BLV-binding receptor. Notably, the BLV RBD does not interact with the Glut1 HTLV receptor, demonstrating that these two closely related retroviruses use different cell receptors for entry, as suggested in an earlier study (33). Using our BLV RBD immunoadhesin, we were able, for the first time, to monitor the BLV-binding receptor on various cell types of different species.…”

supporting

confidence: 60%

Cell Surface Expression of the Bovine Leukemia Virus-Binding Receptor on B and T Lymphocytes Is Induced by Receptor Engagement

Lavanya

Kinet

Montel‐Hagen

et al. 2008

The Journal of Immunology

View full text Add to dashboard Cite

Bovine leukemia virus (BLV), one of the most common infectious viruses of cattle, is endemic in many herds. Approximately 30–40% of adult cows in the United States are infected by this oncogenic C-type retrovirus and 1–5% of animals will eventually develop a malignant lymphoma. BLV, like the human and simian T cell leukemia viruses, is a deltaretrovirus but, in contrast with the latter, the BLV receptor remains unidentified. In this study, we demonstrate that the amino-terminal 182 residues of the BLV envelope glycoprotein surface unit encompasses the receptor-binding domain. A bona fide interaction of this receptor-binding domain with the BLV receptor was demonstrated by specific interference with BLV, but not human T cell leukemia virus, envelope glycoprotein-mediated binding. We generated a rabbit Ig Fc-tagged BLV receptor-binding domain construct and ascertained that the ligand binds the BLV receptor on target cells from multiple species. Using this tool, we determined that the BLV-binding receptor is expressed on differentiating pro/pre-B cells in mouse bone marrow. However, the receptor was not detected on mature/quiescent B cells but was induced upon B cell activation. Activation of human B and T lymphocytes also induced surface BLV-binding receptor expression and required de novo protein synthesis. Receptor levels were down-regulated as activated lymphocytes returned to quiescence. In the human thymus, BLV-binding receptor expression was specifically detected on thymocytes responding to the IL-7 cytokine. Thus, expression of the BLV-binding receptor is a marker of enhanced metabolic activity in B cells, T cells, and thymocytes.

show abstract

“…These Env use distinct cellular receptors, all of which are found on a wide variety of human cell types. 11 MLV vectors bearing these Env have been used for the transduction of primary cells such as lymphocytes and CD34 þ progenitor cells in both preclinical [12][13][14] and clinical [15][16][17][18] applications.…”

Section: Introductionmentioning

confidence: 99%

Characterization of HIV-1 vectors with gammaretrovirus envelope glycoproteins produced from stable packaging cells

et al. 2004

View full text Add to dashboard Cite

Receptor interference groups of 20 retroviruses plating on human cells

Cited by 229 publications

References 68 publications

HCMV gH/gL/UL128–131 interferes with virus entry into epithelial cells: Evidence for cell type-specific receptors

HCMV gH/gL/UL128–131 interferes with virus entry into epithelial cells: Evidence for cell type-specific receptors

Cell Surface Expression of the Bovine Leukemia Virus-Binding Receptor on B and T Lymphocytes Is Induced by Receptor Engagement

Characterization of HIV-1 vectors with gammaretrovirus envelope glycoproteins produced from stable packaging cells

Contact Info

Product

Resources

About