Receptor for ATP in the membrane of mammalian sensory neurones

Krishtal, O. A.; Marchenko, S. M.; Pidoplichko, V I

doi:10.1016/0304-3940(83)90524-4

Cited by 318 publications

(160 citation statements)

References 5 publications

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“…Earlier in the same year Krishtal and colleagues reported large inward currents in response to ATP application in neurones from a wide variety of sensory ganglia [8]. These early reports were followed by evidence of both membrane ionic current responses and changes in neurone action potential firing patterns measured in a variety of preparations.…”

Section: Historical Backgroundmentioning

confidence: 96%

ATP ‐ a fast neurotransmitter

1993

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ATP receptor-mediated responses in peripheral and central neurones have many charactenstics which suggest that ATP may act as a fast neurotransmitter. While the receptors underlying these responses have properties which are similar to other ligand-gated ion channels which mediate fast neurotransmision, the nature of their calcium permeability and the rapid breakdown of ATP to adenosine may confer unique properties on ATP mediated synaptic transmission. The evidence that ATP acts as a fast nemotransmitter is reviewed and the properties of ATP and its receptor channels are discussed in terms of synaptic transmission.

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Section: Historical Backgroundmentioning

confidence: 96%

ATP ‐ a fast neurotransmitter

1993

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“…However, the search for some other, possibly more specific, ligands for these channels (in the course of which the extracts of different tissues were applied to the sensory neurons) resulted in a discovery of one more mechanism employing almost as ubiquitous agonist, ATP. ATP-activated conductance was found in sensory neurons [24]. While purinergic mechanisms were already known in smooth muscles [1], this was the first indication on their existence in the nervous tissue.…”

Section: Introductionmentioning

confidence: 90%

“…For this purpose, we used rapid "square-pulse" application techniques combining two separate U-tubes [24] targeted at the surface-attached cell. The rate of solution change was 40-60 ms.…”

Section: Methodsmentioning

confidence: 99%

The agonists for nociceptors are ubiquitous, but the modulators are specific: P2X receptors in the sensory neurons are modulated by cannabinoids

Krishtal

Lozovaya

Fedorenko

et al. 2006

Pflugers Arch - Eur J Physiol

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“…The membrane potential was measured through an Ag-AgCl wire in a 3M KCIagar plug mounted on the suction pipette holder. The reference electrode (also an Ag-AgCI wire) was placed in a polyethylene catheter which was used for rapid solution changes (Krishtal et al, 1983;Akaike et al, 1986). The resistance between the Ag-AgCl wires Wvas less than 10 kohm when the suction pipette was filled with normal internal solution (NIS: 30 kHz and passing current for 25 or 50% of the cycle (Ishizuka et al, 1984).…”

Section: Methodsmentioning

confidence: 99%

Electrophysiological estimation of the actions of acetylcholinesterase inhibitors on acetylcholine receptor and cholinesterase in physically isolated Aplysia neurones

Oyama

Hori

Evans

et al. 1989

British J Pharmacology

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1 The actions of representative cholinesterase inhibitors on the acetylcholine responses of physically isolated single neurones from the pedal ganglion of Aplysia californica were studied, using electrophysiological techniques and rapid agonist application to analyse both the inhibitory actions on the acetylcholine receptor-channel complex and the degree of inhibition of acetylcholinesterase activity on the same neurone. The inhibitors used were physostigmine, edrophonium and diisopropylfluorophosphate (DFP). 2 When selected neurones were suddenly exposed to 50 JM acetylcholine by a 'concentration clamp' technique a large Na-dependent inward current was initiated, and decayed in the continued presence of acetylcholine without external perfusion. However, if perfusion of the acetylcholine solution was reinitiated the current increased somewhat, indicating that the decay of current was due to some combination of receptor desensitization and local depletion of acetylcholine at the membrane by acetylcholinesterase. 3 With simultaneous application of acetylcholine (50!M) and physostigmine (0.1 to 100l M) there was a dose-dependent reduction of peak amplitude of the acetylcholine response. However, physostigmine at low concentrations (0.1 to 10.uM) caused a time-dependent increase in the current amplitude alone with a time-and dose-dependent inhibition of acetylcholinesterase activity. At the highest concentration of physostigmine (100 uM) acetylcholinesterase activity was abolished but the current peak was very depressed. After removal of physostigmine from the bathing solution, the current amplitude decreased toward the control at the two lower concentrations as the inhibitory actions on acetylcholinesterase activity were almost reversible, while at the two higher concentrations (10 and 100pM) the current increased and the inhibition of acetylcholinesterase remained. 4 When acetylcholine (50pM) and edrophonium (0.1 to 10pM) were applied simultaneously, edrophonium caused a dose-dependent increase in the peak amplitude that was correlated with a dosedependent inhibition of acetylcholinesterase activity. Prolonged exposure to edrophonium did not change the peak amplitude and there was no time-dependent change in the inhibition of acetylcholinesterase activity. At the highest concentration of edrophonium used (100pM), simultaneous application with acetylcholine augmented the peak amplitude relative to control, but to a lesser extent than 10 gM. Prolonged exposure to the highest concentration of edrophonium caused a timedependent reduction in the peak amplitude. The effects of edrophonium were quickly reversible after the removal of the drug from the bathing solution. 5 DFP (1 and 10mM), similar to 1OOpM physostigmine, caused a dramatic reduction of the peak current on simultaneous application with ftetylcholine. During exposure to DFP the current amplitude and acetylcholinesterase activity were very depressed. After removing DFP from the bathing solution the current amplitude increased to more than the control level a...

show abstract

Receptor for ATP in the membrane of mammalian sensory neurones

Cited by 318 publications

References 5 publications

ATP ‐ a fast neurotransmitter

ATP ‐ a fast neurotransmitter

The agonists for nociceptors are ubiquitous, but the modulators are specific: P2X receptors in the sensory neurons are modulated by cannabinoids

Electrophysiological estimation of the actions of acetylcholinesterase inhibitors on acetylcholine receptor and cholinesterase in physically isolated Aplysia neurones

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