Receptor- and store-operated mechanisms of calcium entry during the nanosecond electric pulse-induced cellular response

Tolstykh, Gleb P.; Cantu, Jody C.; Tarango, Melissa; Ibey, Bennett L.

doi:10.1016/j.bbamem.2018.12.007

Cited by 9 publications

(7 citation statements)

References 83 publications

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“…The nsPEF effects on Ca 2+ activation in CHO cells have been studied in detail earlier, with FURA-2 and Calcium Green dyes [8,12,33,37,42]. Here, we observed a similar response using Calbryte dye, and compared it side by side to the nsPEF effect when 2 mM of Ca 2+ was substituted for 2 mM Ba 2+ ( Figure 2).…”

Section: Int J Mol Sci 2019 20 X; Doi: For Peer Reviewwwwmdpisupporting

confidence: 74%

“…Cytosolic Ca 2+ level is tightly controlled, hence, in quiescent cells, the dye fluorescence can be stable for a long time. However, Ca 2+ entry through nanopores should be separated from the activation of cation channels which can admit Ca 2+ [24,29,[34][35][36], as well as from receptor-and store-operated Ca 2+ entry [37]. Moreover, a modest Ca 2+ entry through the electropermeabilized membrane may trigger an overwhelmingly strong response by activating calcium-induced calcium release (CICR), followed by massive active pumping of excess Ca 2+ from the cytosol [33].…”

Section: Introductionmentioning

confidence: 99%

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Probing Nanoelectroporation and Resealing of the Cell Membrane by the Entry of Ca2+ and Ba2+ Ions

Šilkūnas

Mangalanathan

et al. 2020

IJMS

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The principal bioeffect of the nanosecond pulsed electric field (nsPEF) is a lasting cell membrane permeabilization, which is often attributed to the formation of nanometer-sized pores. Such pores may be too small for detection by the uptake of fluorescent dyes. We tested if Ca2+, Cd2+, Zn2+, and Ba2+ ions can be used as nanoporation markers. Time-lapse imaging was performed in CHO, BPAE, and HEK cells loaded with Fluo-4, Calbryte, or Fluo-8 dyes. Ca2+ and Ba2+ did not change fluorescence in intact cells, whereas their entry after nsPEF increased fluorescence within <1 ms. The threshold for one 300-ns pulse was at 1.5–2 kV/cm, much lower than >7 kV/cm for the formation of larger pores that admitted YO-PRO-1, TO-PRO-3, or propidium dye into the cells. Ba2+ entry caused a gradual emission rise, which reached a stable level in 2 min or, with more intense nsPEF, kept rising steadily for at least 30 min. Ca2+ entry could elicit calcium-induced calcium release (CICR) followed by Ca2+ removal from the cytosol, which markedly affected the time course, polarity, amplitude, and the dose-dependence of fluorescence change. Both Ca2+ and Ba2+ proved as sensitive nanoporation markers, with Ba2+ being more reliable for monitoring membrane damage and resealing.

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Section: Int J Mol Sci 2019 20 X; Doi: For Peer Reviewwwwmdpisupporting

confidence: 74%

Section: Introductionmentioning

confidence: 99%

Probing Nanoelectroporation and Resealing of the Cell Membrane by the Entry of Ca2+ and Ba2+ Ions

Šilkūnas

Mangalanathan

et al. 2020

IJMS

View full text Add to dashboard Cite

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“…Similar to the results of others [ 59 , 60 ], a significantly increased externalization of phosphatidylserine immediately after nsPEF treatment was demonstrated, when compared to the samples stained with fluorophores after nsPEF exposure and measured at 30 min. The first phenomenon was likely caused by membrane permeabilization, especially since the influx of Ca 2+ from the external environment, as well as the release of calcium ions from the intracellular stores caused by nsPEF [ 7 ], make linking PS with AnV easier [ 61 ]. Some researchers also believe that negatively charged PS can be pushed from the inner to the outer leaflet of the membrane driven by an electric potential across the membrane [ 31 ].…”

Section: Discussionmentioning

confidence: 99%

“…Among the effects of nsPEF, cell swelling and blebbing, the activation of protein kinase pathways, cell cycle arrest in the G2/M phase and, above all, electroporation of the plasma and intracellular membranes of organelles leading to apoptosis or necrosis, have already been documented [ 7 , 8 , 9 , 10 ]. Various mammalian cells in vitro showed different levels of sensitivity to the treatment [ 11 , 12 ].…”

Section: Introductionmentioning

confidence: 99%

Nanosecond Pulsed Electric Field Only Transiently Affects the Cellular and Molecular Processes of Leydig Cells

Kasprzycka

Trębińska-Stryjewska

Lewandowski

et al. 2021

IJMS

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The purpose of this study was to verify whether the nanosecond pulsed electric field, not eliciting thermal effects, permanently changes the molecular processes and gene expression of Leydig TM3 cells. The cells were exposed to a moderate electric field (80 quasi-rectangular shape pulses, 60 ns pulse width, and an electric field of 14 kV/cm). The putative disturbances were recorded over 24 h. After exposure to the nanosecond pulsed electric field, a 19% increase in cell diameter, a loss of microvilli, and a 70% reduction in cell adhesion were observed. Some cells showed the nonapoptotic externalization of phosphatidylserine through the pores in the plasma membrane. The cell proportion in the subG1 phase increased by 8% at the expense of the S and G2/M phases, and the DNA was fragmented in a small proportion of the cells. The membrane mitochondrial potential and superoxide content decreased by 37% and 23%, respectively. Microarray’s transcriptome analysis demonstrated a negative transient effect on the expression of genes involved in oxidative phosphorylation, DNA repair, cell proliferation, and the overexpression of plasma membrane proteins. We conclude that nanosecond pulsed electric field affected the physiology and gene expression of TM3 cells transiently, with a noticeable heterogeneity of cellular responses.

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“…Stromal interaction molecule 1 (STIM1) is oligomerized when Ca 2+ stores are depleted triggering ORAI1-mediated Ca 2+ entry (with cyclophilin A as positive regulator) [ 96 ]. TRPC1 and ORAI1 could even be coupled [ 102 ].…”

Section: Cytosolic Ion Fluxes In Procoagulant Coat Plateletsmentioning

confidence: 99%

Mechanisms Underlying Dichotomous Procoagulant COAT Platelet Generation—A Conceptual Review Summarizing Current Knowledge

Veuthey

Aliotta

Calderara

et al. 2022

IJMS

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Procoagulant platelets are a subtype of activated platelets that sustains thrombin generation in order to consolidate the clot and stop bleeding. This aspect of platelet activation is gaining more and more recognition and interest. In fact, next to aggregating platelets, procoagulant platelets are key regulators of thrombus formation. Imbalance of both subpopulations can lead to undesired thrombotic or bleeding events. COAT platelets derive from a common pro-aggregatory phenotype in cells capable of accumulating enough cytosolic calcium to trigger specific pathways that mediate the loss of their aggregating properties and the development of new adhesive and procoagulant characteristics. Complex cascades of signaling events are involved and this may explain why an inter-individual variability exists in procoagulant potential. Nowadays, we know the key agonists and mediators underlying the generation of a procoagulant platelet response. However, we still lack insight into the actual mechanisms controlling this dichotomous pattern (i.e., procoagulant versus aggregating phenotype). In this review, we describe the phenotypic characteristics of procoagulant COAT platelets, we detail the current knowledge on the mechanisms of the procoagulant response, and discuss possible drivers of this dichotomous diversification, in particular addressing the impact of the platelet environment during in vivo thrombus formation.

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Receptor- and store-operated mechanisms of calcium entry during the nanosecond electric pulse-induced cellular response

Cited by 9 publications

References 83 publications

Probing Nanoelectroporation and Resealing of the Cell Membrane by the Entry of Ca2+ and Ba2+ Ions

Probing Nanoelectroporation and Resealing of the Cell Membrane by the Entry of Ca2+ and Ba2+ Ions

Nanosecond Pulsed Electric Field Only Transiently Affects the Cellular and Molecular Processes of Leydig Cells

Mechanisms Underlying Dichotomous Procoagulant COAT Platelet Generation—A Conceptual Review Summarizing Current Knowledge

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