Receptor Activator of Nuclear Factor‐κB Ligand and Sclerostin Expression in Osteocytes of Alveolar Bone in Rats With Ligature‐Induced Periodontitis

Kim, Ji Hye; Lee, Dong‐Eun; Heon, Jeong; Bak, Eun‐Jung; Yoo, Yun‐Jung

doi:10.1902/jop.2014.140230

Cited by 37 publications

(40 citation statements)

References 40 publications

(91 reference statements)

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“…In this study, ABL similarly increased at day 3 after ligature placement in the P and DP groups. Consistent with the authors’ previous study, 10 ABL in the P group did not further increase at day 20 after ligature placement. However, the ABL observed in the DP group increased more than that observed in the P group at day 20, and the increase was continual from ligature to day 20.…”

Section: Discussionsupporting

confidence: 92%

“…At day 3 after ligature placement, when osteoid formation markedly decreased in both groups, sclerostin expression increased. At day 20 after ligature placement, when osteoid formation in the P group notably increased, sclerostin expression decreased, which was also observed in the authors’ previous study 10 . Interestingly, increased sclerostin expression in the DP group was sustained until day 20 after ligature placement, at which time diminished bone formation was also observed.…”

Section: Discussionsupporting

confidence: 85%

“…The osteoid area in the distal area of the first molar was measured to examine new bone formation. Osteoid formation was measured as the area of unmineralized bone matrix (light pink color) between osteoblasts (cuboidal bone‐lining cells) and the mineralized bone surface, which was stained a dark pink color 10 . The osteoid area, measured in a region of interest (ROI) that extended 0.5 mm from the ABC in the distal area of the first molar, was examined under ×20 magnification using the imaging software.…”

Section: Methodsmentioning

confidence: 99%

See 2 more Smart Citations

Osteocytic Sclerostin Expression in Alveolar Bone in Rats With Diabetes Mellitus and Ligature‐Induced Periodontitis

Kim¹,

Lee²,

Woo³

et al. 2015

Journal of Periodontology

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Enhanced alveolar bone loss, suppressed bone formation, and prevalent TNF-α expression were characteristic of the DP group compared with the P group. Suppressed bone formation in the DP group was observed simultaneously with increased sclerostin and TNF-α expression. These results suggest that upregulated osteocytic sclerostin expression in periodontitis accompanied by DM may play a role in suppressed bone formation.

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Section: Discussionsupporting

confidence: 92%

Section: Discussionsupporting

confidence: 85%

Section: Methodsmentioning

confidence: 99%

See 1 more Smart Citation

Osteocytic Sclerostin Expression in Alveolar Bone in Rats With Diabetes Mellitus and Ligature‐Induced Periodontitis

Kim¹,

Lee²,

Woo³

et al. 2015

Journal of Periodontology

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“…A study on ligature‐induced periodontitis in rats demonstrated an increased expression of osteocytic SOST, which correlated with suppressed osteoid formation. Increased amounts of RANKL expression was also correlated with the accumulation of osteoclasts . In addition to its anti‐anabolic effects, SOST also acts through the Wnt/β‐catenin pathway and increases RANKL, resulting in bone resorption.…”

Section: Discussionmentioning

confidence: 94%

Differential expression of periostin, sclerostin, receptor activator of nuclear factor‐κB, and receptor activator of nuclear factor‐κB ligand genes in severe chronic periodontitis

Sankardas

Lavu

Lakakula

et al. 2018

J of Invest & Clin Dent

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Aim The aim of the present study was to determine and compare the expression profile of periostin (POSTN), sclerostin (SOST), receptor activator nuclear factor‐κB (RANK), and RANK ligand (RANKL) genes in gingival tissue samples collected from healthy gingiva (control) and severe chronic periodontitis sites. Methods Fifty systemically‐healthy individuals was enrolled in the present case‐control study. Gingival tissue samples were obtained from healthy gingiva (N = 25) and sites with severe chronic periodontitis (N = 25). Total RNA was isolated from all the tissues. cDNA conversion was then performed using a reverse transcription polymerase chain reaction (PCR) program. Real‐time PCR and SYBR green method were used to determine the expression levels of SOST, POSTN, RANK, and RANKL genes. Results An elevated expression (3.5‐4‐fold) of SOST, RANK, and RANKL genes, with a concomitant reduced expression of the POSTN gene, was identified in severe chronic periodontitis. The intergroup difference between the mean delta cyclic threshold values showed statistical significance at P<.001. Conclusions The expression profile of SOST, RANK, RANKL, and POSTN genes observed in gingival tissue samples from sites with severe chronic periodontitis and healthy gingiva suggests that the differential level of the gene expression could serve as an indicator of periodontitis progression/severity.

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“…In pathological conditions, sclerostin expression is altered in response to inflammatory molecules such as TNFα. Recently, a study using a rat model of ligature‐induced periodontitis revealed significant increases in sclerostin expression in osteocytes during the early and destructive phase of the disease . Interestingly, the rise in sclerostin levels was associated with a similar increase in RANKL production.…”

Section: Effects Of Tumour Necrosis Factor‐α On Osteocytes and Their mentioning

confidence: 99%

The effects of tumour necrosis factor‐α on bone cells involved in periodontal alveolar bone loss; osteoclasts, osteoblasts and osteocytes

Algate

Haynes

Bartold

et al. 2015

J of Periodontal Research

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Periodontitis is the most common bone loss pathology in adults and if left untreated is responsible for premature tooth loss. Cytokines, such as tumour necrosis factor-α (TNFα), involved in the chronic inflammatory response within the periodontal gingiva, significantly influence the normal bone remodelling processes. In this review, the effects of TNFα on bone metabolism in periodontitis are evaluated in relation to its direct and indirect actions on bone cells including osteoclasts, osteoblasts and osteocytes. Evidence published to date suggests a potent catabolic role for TNFα through the stimulation of osteoclastic bone resorption as well as the suppression of osteoblastic bone formation and osteocytic survival. However, the extent and timing of TNFα exposure in vitro and in vivo greatly influences its effect on skeletal cells, with contradictory anabolic activity observed with TNFα in a number of studies. None the less, it is evident that managing the chronic inflammatory response in addition to the deregulated bone metabolism is required to improve periodontal and inflammatory bone loss treatments‬‬‬‬‬‬‬‬‬‬‬‬‬‬‬‬‬‬‬‬‬‬‬‬‬‬‬‬‬‬‬‬‬‬‬‬‬‬‬.

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Receptor Activator of Nuclear Factor‐κB Ligand and Sclerostin Expression in Osteocytes of Alveolar Bone in Rats With Ligature‐Induced Periodontitis

Cited by 37 publications

References 40 publications

Osteocytic Sclerostin Expression in Alveolar Bone in Rats With Diabetes Mellitus and Ligature‐Induced Periodontitis

Osteocytic Sclerostin Expression in Alveolar Bone in Rats With Diabetes Mellitus and Ligature‐Induced Periodontitis

Differential expression of periostin, sclerostin, receptor activator of nuclear factor‐κB, and receptor activator of nuclear factor‐κB ligand genes in severe chronic periodontitis

The effects of tumour necrosis factor‐α on bone cells involved in periodontal alveolar bone loss; osteoclasts, osteoblasts and osteocytes

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