Receptor activated C kinase is down-regulated in the male gonad of the marine bivalve mollusc Mya arenaria exposed to tributyltin (TBT)

Spreading by snail (Lymnaea stagnalis) defence cells is regulated through integrated PKC, FAK and Src signalling

“…During sustained spreading, PKCs remain activated but the role of the PKCα-like protein in the maintenance of focal adhesions is reduced integrin β chains (Besson et al 2002). Although RACK1 has not yet been identified/characterised in L. stagnalis, the amino acid sequences of RACK1 from B. glabrata (Lardans et al 1998) and the bivalve mollusc Mya arenaria (Siah et al 2007) are highly conserved with that of vertebrates; indeed, M. arenaria RACK1 shares 89.7 and 72.1% sequence homology with RACK1 from chicken and human, respectively. Importantly, the putative Src-binding site in human RACK1 (Tyr246; Chang et al 2001) appears conserved in mollusc RACK1 together with the PKC interaction motif and PKC phosphorylation sites.…”

Effects of pesticide compounds (chlorothalonil and mancozeb) and benzo[a]pyrene mixture on aryl hydrocarbon receptor, p53 and ubiquitin gene expression levels in haemocytes of soft-shell clams (Mya arenaria)

“…For an accurate quantification of transcript levels, recent studies performed in M. arenaria have shown the importance of selecting an appropriate set of housekeeping genes to normalize data related to gene expression levels in haemocytes (Araya et al 2008;Siah et al 2008b). Four reference genes (ribosomal proteins S18 and L37 and the elongation factors EF1 and EF2) usually used as housekeeping genes were selected from EST clones obtained by subtractive suppressive hybridization performed in M. arenaria (Siah et al 2007). Using geNorm open source program, the stability of gene expression levels related to contamination condition has shown that Rp S18 and Rp L37 could be used as housekeeping genes.…”

“…Based on the expressed sequence tags (EST), clones obtained by subtractive suppressive hybridization previously performed in M. arenaria (Siah et al 2007), the forward and reverse primers were designed in order to obtain a unique amplification product using ''Primer 3'' software (Table 1).…”

Molecular cloning, characterization and expression analysis of receptor for activated C kinase 1 (RACK1) from pearl oyster (Pinctada martensii) challenged with bacteria and exposed to cadmium

“…So far, RACK1 has been cloned from a variety of species ranging from parasite to human [18e20]. In mollusk, RACK1 was only identified from soft-shell clams (Mya arenaria), showing that RACK1 is down-regulated in the male gonad exposed to tributyltin [21]. However, to our knowledge, whether molluscan RACK1 is involved in host defence response remained unknown to date.…”