Receptive Field Sizes of Nyxnob Mouse Retinal Ganglion Cells

Abstract

Patients with congenital nystagmus, involuntary eye movements, often have a reduced visual acuity. Some of these patients have a retinal-specific mutation in the protein nyctalopin, which is also present in the Nyxnob mouse. In these mice, retinal ganglion cells (RGCs) have oscillatory activity, which leads to expanded axonal projections towards the dLGN and consequently to a desegregation of retinal projections to the brain. In this study, we investigate whether the receptive fields of Nyxnob RGCs have also expanded by measuring the size of their receptive fields using MEA recordings. Contrary to our expectation, relative to wild-type (WT) mice we found receptive field sizes in the Nyxnob retina had not increased but instead had decreased for green-light preferring RGCs. Additionally, we also found the receptive fields of UV-light preferring RGCs are larger than green-light preferring RGCs in both WT and Nyxnob mice.

A Common Cause for Nystagmus in Different Congenital Stationary Night Blindness Mouse Models

“…Multielectrode RGC recordings were performed as described previously (Winkelman et al, 2019;Hölzel et al, 2022). After at least one hour of dark adaptation, mice were sedated using a mixture of CO2/O2 and ultimately euthanized by cervical dislocation.…”

A common cause for nystagmus in different congenital stationary night blindness mouse models

“…Retinal dissection. Multielectrode RGC recordings were performed as described previously (Hölzel et al, 2022;Winkelman et al, 2019). After 60-75 min of dark adaptation, mice were sedated using a mixture of CO 2 /O 2 and ultimately killed by cervical dislocation.…”