Receptive field center size decreases and firing properties mature in ON and OFF retinal ganglion cells after eye opening in the mouse

Koehler, Christopher L.; Akimov, Nikolay P.; Renterı́a, René C.

doi:10.1152/jn.01046.2010

Cited by 35 publications

(43 citation statements)

References 41 publications

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“…Their spectral behavior is similar to that of most murine RGCs (Ekesten et al, 2000; Wang et al, 2011) with excitatory drive from both S and M cone opsins but enhanced S-cone input in the ventral retina. Their receptive fields are large, with center diameters ~6-10 degrees of visual angle, among the largest observed among murine RGCs (Koehler et al, 2011). This corresponds well with their dendritic-field dimensions, as has been observed in ganglion cells generally and alpha-like cells in particular (Peichl, 1991).…”

Section: Discussionmentioning

confidence: 99%

Form and Function of the M4 Cell, an Intrinsically Photosensitive Retinal Ganglion Cell Type Contributing to Geniculocortical Vision

et al. 2012

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The photopigment melanopsin confers photosensitivity upon a minority of retinal output neurons. These intrinsically photosensitive retinal ganglion cells (ipRGCs) are more diverse than once believed, comprising five morphologically distinct types, M1 through M5. Here, in mouse retina, we provide the first in-depth characterization of M4 cells, including their structure, function and central projections. M4 cells apparently correspond to ON alpha cells of earlier reports, and are easily distinguished from other ipRGCs by their very large somata. Their dendritic arbors are more radiate and highly branched than those of M1, M2 or M3 cells. The melanopsin-based intrinsic photocurrents of M4 cells are smaller than those of M1 and M2 cells, presumably because melanopsin is more weakly expressed; we can detect it immunohistochemically only with strong amplification. Like M2 cells, M4 cells exhibit robust, sustained synaptically-driven ON responses and dendritic stratification in the ON-sublamina of the IPL. However, their stratification patterns are subtly different, with M4 dendrites positioned just distal to those of M2 cells and just proximal to the ON cholinergic band. M4 receptive fields are large, with an ON center, antagonistic OFF surround, and non-linear spatial summation. Their synaptically-driven photoresponses lack direction selectivity and show higher ultraviolet sensitivity in the ventral retina than in the dorsal retina, echoing the topographic gradient in S- and M-cone opsin expression. M4 cells are readily labeled by retrograde transport from the dorsal lateral geniculate nucleus, and thus likely contribute to the pattern vision that persists in mice lacking functional rods and cones.

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Section: Discussionmentioning

confidence: 99%

Form and Function of the M4 Cell, an Intrinsically Photosensitive Retinal Ganglion Cell Type Contributing to Geniculocortical Vision

et al. 2012

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“…Spike waveforms recorded by each electrode were sorted into single units in Offline Sorter (Plexon, Dallas, TX) (Koehler et al 2011;Nirenberg et al 2001;Tian and Copenhagen 2003). Briefly, a threshold of 68 V was used to detect action potentials, the first and second principal components calculated, and clusters formed with action potentials having similar principal components (Elstrott et al 2008;Tian and Copenhagen 2003).…”

Section: Methodsmentioning

confidence: 99%

Subtype-dependent postnatal development of direction- and orientation-selective retinal ganglion cells in mice

Chen

Liu

Tian

2014

Journal of Neurophysiology

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Chen H, Liu X, Tian N. Subtype-dependent postnatal development of direction-and orientation-selective retinal ganglion cells in mice. J Neurophysiol 112: 2092J Neurophysiol 112: -2101J Neurophysiol 112: , 2014. First published August 6, 2014; doi:10.1152/jn.00320.2014.-The direction-selective ganglion cells (DSGCs) and orientation-selective ganglion cells (OSGCs) encode the directional and the orientational information of a moving object, respectively. It is unclear how DSGCs and OSGCs mature in the mouse retina during postnatal development. Here we investigated the development of DSGCs and OSGCs after eye-opening. We show that 1) DSGCs and OSGCs are present at postnatal day 12 (P12), just before eye-opening; 2) the fractions of both DSGCs and OSGCs increase from P12 to P30; 3) the development of DSGCs and OSGCs is subtype dependent; and 4) direction and orientation selectivity are two separate features of retinal ganglion cells (RGCs) in the mouse retina. We classified RGCs into different functional subtypes based on their light response properties. Compared with P12, the direction and orientation selectivity of ON-OFF RGCs but not ON RGCs became stronger at P30. The tuning width of DSGCs for both ON and ON-OFF subtypes decreased with age. For OSGCs, we divided them into non-direction-selective (non-DS) OSGCs and direction-selective OSGCs (DS&OSGCs). For DS&OSGCs, we found that there was no correlation between the direction and orientation selectivity, and that the tuning width of both ON and ON-OFF subtypes remained unchanged with age. For non-DS OSGCs, the tuning width of ON but not ON-OFF subtype decreased with development. These findings provide a foundation to reveal the molecular and synaptic mechanisms underlying the development of the direction and orientation selectivity in the retina.retinal ganglion cell; multielectrode array; direction-selective ganglion cell; orientation-selective ganglion cell

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“…Linear RF studies have provided insight into space-time processing of the center, but the surround has often been ignored, partially due to inability to detect it (Kerschensteiner et al, 2008; Koehler et al, 2011). A recent study in mouse found that blockage of the ON crossover pathway did not alter the chromatic surround of JamB-RGCs (J-RGCs) which was proposed to be driven primarily by horizontal cells (Joesch and Meister, 2016).…”

Section: Resultsmentioning

confidence: 99%

“…We used white noise mapping and calculated the geometric mean, similar to other reports and our normal sizes are within their reported measurements (Della Santina et al, 2013). Other reports used different stimulation protocols (gratings, spots, etc) and different calculation methods (hypotenuse, area; Koehler et al, 2011). …”

Section: Discussionmentioning

confidence: 99%

The ON Crossover Circuitry Shapes Spatiotemporal Profile in the Center and Surround of Mouse OFF Retinal Ganglion Cells

Sabharwal

Seilheimer

Cowan

et al. 2016

Front. Neural Circuits

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Retinal ganglion cells (RGCs) are often grouped based on their functional properties. Many of these functional properties, such as receptive field (RF) size, are driven by specific retinal circuits. In this report, we determined the role of the ON bipolar cell (BC) mediated crossover circuitry in shaping the center and surround of OFF RGCs. We recorded from a large population of mouse RGCs using a multielectrode array (MEA) while pharmacologically removing the ON BC-mediated crossover circuit. OFF sustained and transient responses to whole field stimuli are lost under scotopic conditions, but maintained under photopic conditions. Though photopic light responses were grossly maintained, we found that photopic light response properties were altered. Using linear RF mapping, we found a significant reduction in the antagonistic surround and a decrease in size of the RF center. Using a novel approach to separate the distinct temporal filters present in the RF center, we see that the crossover pathway contributes specifically to the sluggish antagonistic filter in the center. These results provide new insight into the role of crossover pathways in driving RGCs and also demonstrate that the distinct inputs driving the RF center can be isolated and assayed by RGC activity.

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Receptive field center size decreases and firing properties mature in ON and OFF retinal ganglion cells after eye opening in the mouse

Abstract: Koehler CL, Akimov NP, Rentería RC. Receptive field center size decreases and firing properties mature in ON and OFF retinal ganglion cells after eye opening in the mouse.

Cited by 35 publications

References 41 publications

Form and Function of the M4 Cell, an Intrinsically Photosensitive Retinal Ganglion Cell Type Contributing to Geniculocortical Vision

Form and Function of the M4 Cell, an Intrinsically Photosensitive Retinal Ganglion Cell Type Contributing to Geniculocortical Vision

Subtype-dependent postnatal development of direction- and orientation-selective retinal ganglion cells in mice

The ON Crossover Circuitry Shapes Spatiotemporal Profile in the Center and Surround of Mouse OFF Retinal Ganglion Cells

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