Recent tuberculosis diagnosis toward the end TB strategy

Cheon, Seon Ah; Cho, Hyun Hee; Kim, Jeonghyo; Lee, Jaebeom; Kim, Hwa Jung; Park, Tae Jung

doi:10.1016/j.mimet.2016.02.007

Cited by 41 publications

(60 citation statements)

References 93 publications

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“…As we know that, there is a lower bumber of bacilli in (+1) AFB rather than (+2 ) or (+3) AFB. 2,11 This condition can lead the toxic effect of bleach becomes greater to the bacilli, and can kill more numerous bacilli than in the sputum with (+2) or (+3) AFB. The mode of how bleach can effectively kill contaminant microorganism in TB culture by initiating oxidative stress to bacterial proteins.…”

Section: Resultsmentioning

confidence: 99%

The Evaluations of Bleach as Decontaminant Solution to Promote The Positivity Rate of Mycobacterium Tuberculosis Culture for Sputum Specimen

Suwarsono¹,

Sjahrurachman²,

Karuniawati³

2017

Proceedings of the 1st International Integrative Conference on Health, Life and Social Sciences (ICHLaS 2017)

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Abstract-Background: Tuberculosis (TB) is still worldwide health problems. Sputum is contaminated by normal flora, that make its should be decontaminated prior to culture. Bleach known as a potent disinfectant, easy to access because it is commercially available, could be the alternative solution as decontaminant in TB culture. Aims Assessing the capability of bleach to promote the positivity rate in TB culture compared by others decontaminant solution. Method We use 35 sputum samples with volume 3-5 ml. The research was conducted at TB laboratory of Faculty of Medicine University of Indonesia, by using 35 samples sputum with positive AFB, 3-5 ml. Each sample was divided into 4 groups that were decontaminated by different methods. The methods are 4%NaOH, 2%NALC-NaOH, 5% oxalic acid and 1% bleach. 1% bleach was prepared from commercially bleach. The positive culture was validated using MPT 64. The culture positivity rate was compared based on its AFB score. Result Bleach had the same 100% positivity rate as the 4% NaOH and NALC for (+2) and (+3) AFB. However, its became smaller rate for the (+1) AFB group, which was 88% for bleach and 96% for NALC and 100% for NaOH. Oxalic acid had the smallest positivity rate in all AFB score compared to the others. Conclusion The positivity rate of the bleach as same as 4% NaOH and NALC for AFB score higher than (+2)

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Section: Resultsmentioning

confidence: 99%

The Evaluations of Bleach as Decontaminant Solution to Promote The Positivity Rate of Mycobacterium Tuberculosis Culture for Sputum Specimen

Suwarsono¹,

Sjahrurachman²,

Karuniawati³

2017

Proceedings of the 1st International Integrative Conference on Health, Life and Social Sciences (ICHLaS 2017)

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“…Furthermore, other techniques require various reagents and fluorescent or chemiluminescent labeling for sensing, exhibit low-yields, and may even require further purification steps [22]. Currently, TB is the leading cause of death in people within the most economically productive age-groups and the second deadliest infectious disease in the world [23]. Since diagnosis represents a vital link in the TB control chain, new costeffective detection platforms are required to achieve quality results in a shorter time span [24].…”

Section: Case Of Study: Tuberculosismentioning

confidence: 99%

Detection of Pathogens Using Microfluidics and Biosensors

Lopez‐Barbosa¹,

Campaña²,

JulianaNoguera³

et al. 2018

Biosensing Technologies for the Detection of Pathogens - A Prospective Way for Rapid Analysis

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Point-of-care devices technology are a promising way towards the recognition of pathogens in early-stage diagnosis, which is critical for the success of inexpensive treatments as opposed to the high costs of managing the disease. The integration of immunoassays with read out circuitry allows the implementation of diagnostic devices for their use by untrained personnel, without compromising reliability. In the following chapter, three different biosensors based on lab-on-a-chip (LoC) and microfluidic technologies were designed, assembled and tested for pathogen diagnosis. The devices allowed the effective detection of the human papilloma virus, Mycobacterium tuberculosis and Chagas parasite in shorter times and with smaller sample volumes than those required by current clinical diagnosis techniques. All devices were benchmarked against commercial techniques in terms of cost and time requirement per test.

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“…The performance of the mts90 RPA assay was evaluated by testing clinical samples. A total of 28 clinical samples were collected that were identied as positive by a Biochip test, including sputum (15), bronchoalveolar lavage uid (BALF -9) and lung tissue (4). The mts90 RPA assay could successfully detect MTB from the clinical samples within 20 minutes (Table 3).…”

Section: Performance Evaluation Of Mts90 Rpa Assay With Clinical Samplesmentioning

confidence: 99%

“…So it is worthwhile to develop a simple, rapid, sensitive and low-cost method for diagnosing TB to facilitate the need of healthcare systems in areas with a high TB burden. 2,15 Unlike traditional PCR methods, isothermal amplication can amplify nucleic acids at a low incubation temperature without a precise thermal cycling, possessing the potential to develop point-of-care testing (POCT) application tools. 16,17 A newly developed technique of isothermal nucleic acid amplication, recombinase polymerase amplication (RPA), mainly relies on three enzymes, i.e., recombinase, single-stranded DNA-binding protein (SSB) and strand displacement DNA polymerase, to complete testing in 20 minutes at an optimal working temperature, approximately 37 C to 42 C. 18 The protein-DNA complex formed by recombinase and primers can nd homologous sequences in the target DNA, followed by a chain exchange reaction, and then the DNA synthesis is initiated by strand displacement polymerase.…”

Section: Introductionmentioning

confidence: 99%