Recent trends of polyphasic approach in taxonomy and cyanobacterial diversity

Perona, Elvira; Muñoz-Martín, María Ángeles; Gómez, Esther Berrendero

doi:10.1016/b978-0-323-91202-0.00008-7

Cited by 1 publication

(1 citation statement)

References 210 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Until recently, it was difficult to conduct fundamental and applied studies on thermophilic strains due to the lack of well-described thermophilic isolates that contain complete genomic, taxonomic, physiological, and morphological data. However, as more studies characterizing thermophilic strains have been published, the new genera have been proposed (Perona et al, 2022 ). Simultaneously, thermophilic cyanobacteria share many of the same challenges regarding their taxonomy to their mesophilic counterparts, most notably simple morphology (Komárek and Anagnostidis, 2005 ).…”

Section: Introductionmentioning

confidence: 99%

Polyphasic characterization of a novel hot-spring cyanobacterium Thermocoleostomius sinensis gen et sp. nov. and genomic insights into its carbon concentration mechanism

2023

View full text Add to dashboard Cite

Thermophilic cyanobacteria play a crucial role as primary producers in hot spring ecosystems, yet their microbiological, taxonomic, and ecological characteristics are not extensively studied. This study aimed to characterize a novel strain of thermophilic cyanobacteria, PKUAC-SCTA174 (A174), using a combination of traditional polyphasic methods and modern genomic-based approaches. The study included 16S rRNA-based phylogeny, ITS secondary structure prediction, morphological and habitat analyses, as well as high-quality genome sequencing with corresponding phylogenomic analyses. The results of the 16S rRNA, 16S-23S ITS secondary structure, morphology, and habitat analyses supported the classification of the strain as a member of a novel genus within the family Oculatellaceae, closely related to Albertania and Trichotorquatus. Genomic analysis revealed the presence of a sophisticated carbon-concentrating mechanism (CCM) in the strain, involving two CO2 uptake systems NDH-I3, and NDH-I4, three types of bicarbonate transporters (BCT1, bicA, sbtA,) and two distinct putative carboxysomal carbonic anhydrases (ccaA1 and ccaA2). The expression of CCM genes was investigated with a CO2 shift experiment, indicating varying transcript abundance among different carbon uptake systems. Based on the comprehensive characterization, the strain was delineated as Thermocoleostomius sinensis, based on the botanical code. The study of the complete genome of strain A174 contributes valuable insights into the genetic characteristics of the genus Thermocoleostomius and related organisms and provides a systematic understanding of thermophilic cyanobacteria. The findings presented here offer valuable data that can be utilized for future research in taxogenomics, ecogenomics, and geogenomics.

show abstract

Section: Introductionmentioning

confidence: 99%