Recent trends in the utilization of LAMP for the diagnosis of viruses, bacteria, and allergens in food

Lakshmi, Buddolla Anantha; Kim, Sang-Hyo

doi:10.1016/b978-0-12-821406-0.00027-8

Cited by 9 publications

(7 citation statements)

References 40 publications

(43 reference statements)

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“…Furthermore, the presence of recognized PCR inhibitors such as calcium, fat, or protein in milk necessitates using specific DNA extraction techniques to ensure high-quality findings. Alternative to regular PCR and quantitative (qPCR) procedures, loop-mediated isothermal amplification (LAMP) has been described as a promising molecular tool for quick on-farm diagnostics [ 161 , 162 ] and food pathogen detection [ 163 , 164 , 165 , 166 , 167 ]. This approach is quicker than PCR, less costly, highly selective for the target sequence, and requires less template quality and complicated apparatus.…”

Section: On-farm and Lab Methods For Monitoring Udder Health And Milk...mentioning

confidence: 99%

Udder Health Monitoring for Prevention of Bovine Mastitis and Improvement of Milk Quality

Neculai-Văleanu¹,

Ariton²

2022

Bioengineering

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To maximize milk production, efficiency, and profits, modern dairy cows are genetically selected and bred to produce more and more milk and are fed copious quantities of high-energy feed to support ever-increasing milk volumes. As demands for increased milk yield and milking efficiency continue to rise to provide for the growing world population, more significant stress is placed on the dairy cow’s productive capacity. In this climate, which is becoming increasingly hotter, millions of people depend on the capacity of cattle to respond to new environments and to cope with temperature shocks as well as additional stress factors such as solar radiation, animal crowding, insect pests, and poor ventilation, which are often associated with an increased risk of mastitis, resulting in lower milk quality and reduced production. This article reviews the impact of heat stress on milk production and quality and emphasizes the importance of udder health monitoring, with a focus on the use of emergent methods for monitoring udder health, such as infrared thermography, biosensors, and lab-on-chip devices, which may promote animal health and welfare, as well as the quality and safety of dairy products, without hindering the technological flow, while providing significant benefits to farmers, manufacturers, and consumers.

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Section: On-farm and Lab Methods For Monitoring Udder Health And Milk...mentioning

confidence: 99%

Udder Health Monitoring for Prevention of Bovine Mastitis and Improvement of Milk Quality

Neculai-Văleanu¹,

Ariton²

2022

Bioengineering

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“…60 LAMP is most widely researched among these isothermal techniques for pathogen detection in microfluidic platforms. 58 Microfluidic platforms integrated with LAMP based assays for pathogenic nucleic acid detection have demonstrated POC capabilities in previous works. 58 The reduced reagent volumes compared to laboratory techniques make them conducive to use in resource limited settings, facilitating multiplexed detection assays and reduced assay costs.…”

Section: Colorimetric Detection Of Virusesmentioning

confidence: 97%

“…62 Microfluidic platforms integrated with LAMP based assays for pathogenic nucleic acid detection have demonstrated POC capabilities in previous works. 62 The reduced reagent volumes compared to laboratory techniques make them conducive to use in resource limited settings, facilitating multiplexed detection assays and reduced assay costs. 65 With the onset of the COVID-19 pandemic, SARS-CoV-2 detection has been a research hotspot in the diagnostic community.…”

Section: Colorimetric Detection Of Virusesmentioning

confidence: 97%

Microfluidic-based colorimetric nucleic acid detection of pathogens

et al. 2023

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“…[3] The emerging isothermal amplification techniques, especially loop-mediated isothermal amplification (LAMP), overcome the limitations of conventional PCR. [4][5][6][7][8][9][10][11][12][13][14][15][16][17][18][19][20][21][22][23] LAMP is a protocol for nucleic acid amplification that was first introduced in 2000. [24] It is not affected by inhibitors and operates at the same temperature throughout the assay run, eliminating the need for thermal cycling and high-cost equipment.…”

Section: Introductionmentioning

confidence: 99%

Point‐of‐Care Diagnostic Platforms for Loop‐Mediated Isothermal Amplification

et al. 2022

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The loop‐mediated isothermal amplification (LAMP) method is one of the Nucleic acid amplification tests (NAATs) that allows for the amplification of target regions without using a thermal cycle. With its unique primer design, LAMP ensures the rapid replication of the targeted DNA region with high specificity and high efficiency. LAMP technology is used for diagnostic purposes in pathogen detection due to its ease of use, low cost, and simplicity without requiring complex equipment. A wide range of LAMP diagnostic platforms have been developed for applications in bacteria, virus, and parasitic pathogen detection. Herein, the methodology of LAMP technology and its applications in pathogen detection and SNP genotyping and mutation detection are discussed. Point‐of‐care (PoC) LAMP platforms designed with the principles of microfluidic chip technology, including LAMP‐on‐a‐chip, paper‐based LAMP, and smartphone‐based LAMP applications have been elaborated. LAMP technology represents a fast, robust, and reliable diagnostic platform for point‐of‐care testing.

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Recent trends in the utilization of LAMP for the diagnosis of viruses, bacteria, and allergens in food

Cited by 9 publications

References 40 publications

Udder Health Monitoring for Prevention of Bovine Mastitis and Improvement of Milk Quality

Udder Health Monitoring for Prevention of Bovine Mastitis and Improvement of Milk Quality

Microfluidic-based colorimetric nucleic acid detection of pathogens

Point‐of‐Care Diagnostic Platforms for Loop‐Mediated Isothermal Amplification

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