Recent trends in tapetum research. A cytological and methodological review

Hesse, Michael; Hess, Michael W.

doi:10.1007/978-3-7091-6661-1_12

Cited by 45 publications

(27 citation statements)

References 80 publications

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“…The tapetum often appears to degenerate at an early stage and this may be related to the presence of sterile pollen observed here in several species (Brocchinia reducta, Dyckia hebdingii, Pitcairnia paniculata, Guzmannia madisonii, Tillandsia dura and Aechmea conglomerata), as failure in tapetum development often results in pollen grain malfunction and sterility (Hesse & Hess 1993). Chichiriccò (1989Chichiriccò ( , 1999 examined pollen and tapetal development in Crocus (Iridaceae) which normally has a secretory tapetum.…”

Section: Tapetum Typementioning

confidence: 78%

Microsporogenesis and anther development in Bromeliaceae

Sajo

Furness

Prychid

et al. 2005

Grana

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Section: Tapetum Typementioning

confidence: 78%

Microsporogenesis and anther development in Bromeliaceae

Sajo

Furness

Prychid

et al. 2005

Grana

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“…This technique allows good freezing of larger samples, such as compact tissues without the use of cryoprotectants Moor 1984, Moor 1987). High pressure freezing, followed by freeze substitution or freezefracturing has been successfully applied to stabilize a variety of unfixed plant tissues, without the use of cryoprotectants, like root tips (Craig and Staehelin 1988, Kaeser et al 1989, Kiss et al 1990), root nodules (Studer et al 1992, Berg 1994, and anthers Hess 1993, Hess andHesse 1994 …”

Section: Introductionmentioning

confidence: 99%

Improved ultrastructural preservation ofPetunia andBrassica ovules and embryo sacs by high pressure freezing and freeze substitution

et al. 1997

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Summary. In order to improve the ultrastructural preservation of the female gametophyte of Petunia x hybrida and Brassica napus we tested several cryofixation techniques and compared the results with those of conventional chemical fixation methods. Ovules fixed with glutaraldehyde and osmium tetroxide in the presence or absence of potassium ferrocyanide showed poor cell morphological and ultrastructural preservation. In ovules cryo-fixed by plunging into liquid propane, the cell morphology was well preserved. However, at the ultrastructural level structure-distorting ice crystals were detected in all tissues. Due to the large size of the ovules, cryofixation by plunging in liquid propane is not adequate for ultrastructural studies. In contrast, P. x hybrida and B. napus ovtfles cryo-fixed by high pressure freezing showed improved cell morphological as well as ultrastructural preservation of the embryo sac and the surrounding integumentary tissues. The contrast of the cellular membranes after fi'eeze substitution with 2% osmium tetroxide and 0.1% uranyI acetate in dry acetone was high. At the ultrastructural level, the most prominent improvements were: straight plasma membranes which were appressed to the ceil walls; turgid appearing organeltes with smooth surface contours; minimal extraction of cytoplasmic and extracelluIar substances. In contrast to the chemically fixed ovules, in high pressure frozen ovules numerous microtubules and multivesicular bodies could be distinguished.

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“…Although various types of tapeta are present within angiosperms, two main types are recognised: secretory tapeta, also known as parietal or glandular tapetum, and amoeboid tapeta, also known as periplasmodial, invasive or intrusive tapetum (Pacini, 1997). The distinction between the two types, however, has become less evident (Rowley et al, 1992), and transitional types have been described (Hesse & Hess, 1993). The predominance of the secretory tapetum among basal angiosperms was shown by Furness and Rudall (2001).…”

Section: Tapetum Maturationmentioning

confidence: 99%