Recent trends in aptamer-based nanobiosensors for detection of vascular endothelial growth factors (VEGFs) biomarker: A review

Nourizad, Abolfazl; Golmohammadi, Saeed; Aghanejad, Ayuob; Tohidkia, Mohammad Reza

doi:10.1016/j.envres.2023.116726

Cited by 12 publications

(3 citation statements)

References 110 publications

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“…Optimal specificity, sensitivity and selectivity of electrochemical biosensors, therefore, relies greatly on the choice of affinity ligands employed in the sensor. Biorecognition probes (antibodies, enzymes, and nucleic acids) need enhanced binding affinity for their targets but are limited in specificity and selectivity due to their challenging physical and chemical modification [ 26 ]. While antibodies (or monoclonal antibodies) have achieved impressive success, they suffer from limitations such as high production costs, batch-to-batch variation, and laborious purification methods used during the production.…”

Section: Introductionmentioning

confidence: 99%

Nanoarchitectonics-based electrochemical aptasensors for highly efficient exosome detection

Javed,

Kong,

Mathesh

et al. 2024

Science and Technology of Advanced Materials

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Exosomes, a type of extracellular vesicles, have attracted considerable attention due to their ability to provide valuable insights into the pathophysiological microenvironment of the cells from which they originate. This characteristic implicates their potential use as diagnostic disease biomarkers clinically, including cancer, infectious diseases, neurodegenerative disorders, and cardiovascular diseases. Aptasensors, which are electrochemical aptamers based biosensing devices, have emerged as a new class of powerful detection technology to conventional methods like ELISA and Western analysis, primarily because of their capability for high-performance bioanalysis. This review covers the current research landscape on the detection of exosomes utilizing nanoarchitectonics strategy for the development of electrochemical aptasensors. Strategies involving signal amplification and biofouling prevention are discussed, with an emphasis on nanoarchitectonics-based bio-interfaces, showcasing their potential to enhance sensitivity and selectivity through optimal conduction and mass transport properties. The ongoing challenges to broaden the clinical applications of these biosensors are also highlighted.

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Section: Introductionmentioning

confidence: 99%

Nanoarchitectonics-based electrochemical aptasensors for highly efficient exosome detection

Javed,

Kong,

Mathesh

et al. 2024

Science and Technology of Advanced Materials

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“…Aptamers are artificial short single-stranded oligonucleotides of DNA or RNA selected by Systematic Evolution of Ligands by Exponential Enrichment (SELEX) ( Tuerk and Gold, 1990 ). Aptamer has excellent chemical stability and is not easily limited by the environmental conditions of the biosensing method ( Liu et al, 2022 ; Nourizad et al, 2023 ). Aptamers are linked to complementary DNA or RNA strands by hybridization, a signaling strategy guided by preprogrammed Watson-Crick base-pairing, to form double-stranded structures called duplexed aptamer (DA), which regulate aptamer function ( Nutiu and Li, 2003 ).…”

Section: Introductionmentioning

confidence: 99%

An aptamer and Au/Si CCA based SERS sensor for ultra-sensitive detection of Vimentin during EMT in gastric cancer

Cheng,

Xu,

Yuan

et al. 2023

Front. Bioeng. Biotechnol.

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Introduction: In this study, a surface-enhanced Raman scattering (SERS) sensor based on a functionalized Au/Si cap-cone array (Au/Si CCA) was constructed using the identity-release strategy to detect Vimentin changes during epithelial-mesenchymal transition (EMT) in gastric cancer (GC).Methods: The periodic structure of Au/Si CCA, which can form “hot spots” with high density and regular arrangement, is a substrate with excellent performance. Au/Si CCA was functionalized with aptamers as the capture substrate, and Au nanocubes (AuNCs) were modified with 5-carboxyfluorescein (5-FAM) labelled complementary strand as SERS probe. The capture substrate and SERS probe were assembled by hybridization, and the SERS signal intensity of 5-FAM was greatly enhanced. The binding of Vimentin to the aptamer resulted in a broken connection between the SERS sensor Au/Si CCA array and AuNCs, which resulted in a decrease in the signal intensity of 5-FAM. The identity-release strategy requires only a simple step of reaction to achieve rapid detection of target proteins, which has clinical practicability.Results: Using this protocol, the concentration of Vimentin in GES-1 cells could be successfully detected, and the detection limit was as low as 4.92 pg/mL. Biological experiments of Vincristine, Oncovin (VCR)-treated GES-1 cells effectively mimicked the EMT process, and Vimentin changes during EMT could be accurately detected by this method.Discussion: This study provides a selective, ultra-sensitive and accurate assay for Vimentin detection, which may provide a means for the future detection of EMT process in GC.

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“…Cancer treatment has also been the focus of many aptamer-based studies [ 40 , 41 , 42 , 43 , 44 , 45 , 46 ]. However, other diseases such as neurodegenerative or cardiovascular diseases, among others, have also been targeted in aptamer studies [ 47 , 48 , 49 , 50 , 51 , 52 ]. In addition to the development of aptamers as therapeutic molecules, other notable approaches include the applications of aptamers as biosensors, through the development of aptamer-based devices and platforms for disease diagnosis [ 53 , 54 , 55 , 56 , 57 , 58 , 59 , 60 , 61 , 62 , 63 , 64 ], and as molecular tools to deliver therapeutic drugs to specific target cells, as well as to improve access to malignant molecules whose inactivation is desirable [ 65 , 66 , 67 , 68 , 69 , 70 ].…”

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confidence: 99%

Aptamers’ Potential to Fill Therapeutic and Diagnostic Gaps

Berzal-Herranz,

Romero-López

2024

Pharmaceuticals

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Recent trends in aptamer-based nanobiosensors for detection of vascular endothelial growth factors (VEGFs) biomarker: A review

Cited by 12 publications

References 110 publications

Nanoarchitectonics-based electrochemical aptasensors for highly efficient exosome detection

Nanoarchitectonics-based electrochemical aptasensors for highly efficient exosome detection

An aptamer and Au/Si CCA based SERS sensor for ultra-sensitive detection of Vimentin during EMT in gastric cancer

Aptamers’ Potential to Fill Therapeutic and Diagnostic Gaps

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